A new rapidly growing mycobacterial species, sp. nov., isolated from the indoor walls of a children's day care centre Free

Abstract

Scotochromogenic mycobacterial isolates from water-damaged parts of indoor building materials of a children’s day care centre represented a phenetically and genetically distinct group of strains. A 16S rDNA dendrogram (1243 bp) showed that the closest species to the new strain MA112/96was . Phylogenetic and phenetic analyses (100 characteristics) grouped the new isolates with . , , and . Ribotyping with ll restriction distinguished the 5 isolates from the other 12 most closely related species by the major bands at 6·5–7 kb and 13–15 kb. The cell morphology of the new isolates was typical of mycobacteria, electron microscopy revealed a triple-layered cell wall with an irregular electron-dense outer layer. They grew at 10–37 °C with no growth at 45 °C in 5 d. The gene encoding the secreted 32 kDa protein, specific to mycobacteria, was detected by PCR. The main whole-cell fatty acids were characterized by high tuberculostearic acid 10Me-C (17% at 28 °C), which increased with increasing growth temperature (22% at 37 °C). The other main fatty acids were C 9 and C (21–20% each), followed by, C 9 (14%), C 10 (8%) and also a high amount of C alcohol (9%). -Mycolic acids, keto-mycolates and wax esters were present (C–C), MK-9(H) (90%) and MK-8(H) were the main menaquinones. The cellular phospholipids were phosphatidylethanolamine, phosphatidylinositol, phosphatidyl inositolmannosides and diphosphatidylglycerol. Polyamine content was low. G+C content was 72·9 mol%. The new isolates are proposed as a new species, sp. nov. The type strain is MA112/96(= DSM 44340).

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1999-01-01
2024-03-29
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References

  1. Altenburger P., Käkmpfer P., Akimov V. N., Lubitz W., Busse H.-J. 1997; Polyamine distribution in Actinomycetes with group B peptidoglycan and species of the genera Brevibacterium, Corynebacterium, and Tsukamurella. Int J Syst Bacteriol 47:270–277
    [Google Scholar]
  2. Andersson M., Laukkanen M., Nurmiaho-Lassila E.-L., Rainey F., Niemelä S., Salkinoja-Salonen M. 1995; Bacillus thermo-sphaericus sp. nov. a new thermophilic ureolytic Bacillus isolated from air. Syst Appl Microbiol 18:203–220
    [Google Scholar]
  3. Andersson M. A., Nikulin M., Köljalg U., Andersson M. C., Rainey F., Reijula K., Hintikka E.-L., Salkinoja-Salonen M. 1997; Bacteria, molds, and toxins in water-damaged building materials. Appl Environ Microbiol 63:387–393
    [Google Scholar]
  4. Briglia M., Rainey F. A., Stackebrandt E., Schraa G., Salkinoja-Salonen M. S. 1996; Rhodococcus percolatus sp. nov., a bacterium degrading 2,4,6-trichlorophenol. Int J Syst Bacteriol 46:23–30
    [Google Scholar]
  5. Brosius J., Palmer M. L., Kennedy P. J., Noller H. F. 1978; Complete nucleotide sequence of the 16S ribosomal RNA gene from Escherichia coli. Proc Nati Acad SciUSA 75:4801–4805
    [Google Scholar]
  6. Bruce J. 1996; Automated system rapidly identifies and characterizes microorganisms in food. Food Technol 50:77–81
    [Google Scholar]
  7. Domenech P., Menendez M. C., Garcia M. J. 1994; Restriction fragment length polymorphisms of 16S rRNA genes in the differentiation of fast-growing mycobacterial species. FEMS Microbiol Lett 116:19–24
    [Google Scholar]
  8. Felsenstein J. 1993; phylip (phylogenetic inference package) version 3.5.1. Department of Genetics. University of Washington, Seattle; USA:
    [Google Scholar]
  9. Flannigan B. 1995; Nature, sources and toxicity of pollutants of indoor air. In Indoor Air Quality: a Comprehensive Reference Book. 139–159 Maroni M., Seifert B., Lindvall T. Amsterdam: Elsevier
    [Google Scholar]
  10. Guay D. R. P. 1996; Nontuberculous mycobacterial infections. Ann Pharmacother 30:819–830
    [Google Scholar]
  11. Häggblom M. M., Nohynek L. J., Palleroni N. J., Kronqvist K., Nurmiaho-Lassila E.-L., Salkinoja-Salonen M., Klatte S., Kroppenstedt R. M. 1994; Transfer of polychlorophenol-degrading Rhodococcus chlorophenolicus (Apajalahti et al. 1986) to the genus Mycobacterium as Mycobacterium chloro-phenolicum comb. nov. Int J Syst Bacteriol 44:485–493
    [Google Scholar]
  12. Hirai Y. 1991; Survival of bacteria under dry conditions: from a viewpoint of nosocomial infection. J Hosp Infect 19:191–200
    [Google Scholar]
  13. Ji Y.-E., Colston M. J., Cox R. A. 1994; The ribosomal RNA (rrm) operons of fast-growing mycobacteria : primary and secondary structures and their relation to rrm operons of pathogenic slow-growers. Microbiology 140:2829–2840
    [Google Scholar]
  14. Jukes T. H., Cantor C. R. 1969; Evolution of protein molecules. Mammalian Protein Metabolism21–132 Munro H. N. New York: Academic Press;
    [Google Scholar]
  15. Kämpfer P., Denner E. B. M., Meyer S., Moore E. R. B., Busse H.-J. 1997; Classification of “ Pseudomonas azotocolligans” Anderson 1955, 132, in the genus Sphingomonas as Sphingo-monas trueperi sp. nov. Int J Syst Bacteriol 47:577–583
    [Google Scholar]
  16. Kleespies M., Kroppenstedt R. M., Rainey F. A., Weber L. E., Stackebrandt E. 1996; Mycobacterium hodleri sp. nov., a new member of the fast-growing mycobacteria capable of degrading polycyclic aromatic hydrocarbons. Int J Syst Bacteriol 46:683–687
    [Google Scholar]
  17. Levy-Frebault V. V., Portaels F. 1992; Proposed minimal standards for the genus Mycobacterium and for description of new slowly growing Mycobacterium species. Int J Syst Bacteriol 42:315–323
    [Google Scholar]
  18. Luquin M., Ausina V., Calahorra F. L., Belda F., Barcelo M. G., Celma C., Prats G. 1991; Evaluation of practical chromato-graphic procedures for identification of clinical isolates of mycobacteria. J Clin Microbiol 29:120–130
    [Google Scholar]
  19. Lutz B. 1992; Identification tests for mycobacteria. In Clinical Microbiology Procedures Handbook3.12.1–3.12.27 Isenberg H. D. Washington: DC: American Society for Microbiology;
    [Google Scholar]
  20. Maidak B. L., Larsen N., McCaughey M. J., Overbeek R., Olsen G. J., Fogel K., Blandy J., Woese C. R. 1994; The Ribosomal Database Project. Nucleic Acids Res 22:3485–3487
    [Google Scholar]
  21. Minnikin D. E., Hutchinson I. G., Caldicott A. B., Goodfellow M. 1980; Thin-layer chromatography of methanolysates of mycolic acid-containing bacteria. J Chromatogr 188:221–233
    [Google Scholar]
  22. Minnikin D. E., Minnikin S. M., Parlett J. H., Goodfellow M. 1985; Mycolic acid patterns of some rapidly-growing species of Mycobacterium. Zentbl Bakteriol Hyg A 259:446–460
    [Google Scholar]
  23. Murray R. G. E., Doetsch R. N., Robinow C. F. 1994; Determinative and cytological light microscopy. . In Methods for General and Molecular Bacteriology21–41 Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington: DC : American Society for Microbiology;
    [Google Scholar]
  24. Mylvaganam S., Dennis P. P. 1992; Sequence heterogeneity between the two genes encoding 16S rRNA from the halophilic archaebacterium Haloarcula marismortui. Genetics 130:399–110
    [Google Scholar]
  25. Ninet B., Monod M., Pawlowski E. J., Metral C, Rohner P., Auckenthaler R., Hirschel B. 1996; Two different 16S rRNA genes in a mycobacterial strain. J Clin Microbiol 34:2531–2536
    [Google Scholar]
  26. Peters M., Mueller C., Ruesch-Gerdes S., Seidel C., Gubel U., Pohle H. D., Ruf B. 1995; Isolation of atypical mycobacteria from tap water in hospitals and homes : is this a possible source of disseminated MAC infection in AIDS patients. J Infection 31:39–44
    [Google Scholar]
  27. Rainey F. A., Ward-Rainey N. L., Janssen P. H., Hippe H., Stackebrandt E. 1996; Clostridium paradoxum DSM 7308T contains multiple 16 rRNA genes with heterogeneous intervening sequences. Microbiology 142:2087–2095
    [Google Scholar]
  28. Reznikov M., Leggo J. H., Dawson D. J. 1971; Investigation by seroagglutination of strains of the Mycobacterium intra-cellulare-M. scrofulaceum group from house dusts and sputum in Southeastern Queensland. Am Rev Resp Dis 104:951–953
    [Google Scholar]
  29. Saitou N., Nei M. 1987; The neighbor-joining method : a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  30. Sneath P. H. A., Sokal R. R. 1973; Numerical Taxonomy: the Principles and Practice of Numerical Classification. San Francisco: Freeman
    [Google Scholar]
  31. Soini H., Skurnik M., Liippo K., Tala E., Viljanen M. K. 1992; Detection and identification of mycobacteria by amplification of a segment of the gene coding for the 32-kilodalton protein. J Clin Microbiol 30:2025–2028
    [Google Scholar]
  32. Stackebrandt E., Rainey F. A., Ward-Rainey N. L. 1997; Proposal for a new hierarchic classification system, Actino-bacteria classis nov. Int J Syst Bacteriol 47:479–491
    [Google Scholar]
  33. Tsukamura M., Ichiyama S. 1986; Numerical classification of rapidly growing nonphotochromogenic mycobacteria. Microbiol Immunol 30:863–882
    [Google Scholar]
  34. Tsukamura M., Mizuno S., Murata H., Nemoto H., Yugi H. 1974; A comparative study of mycobacteria from patient's room dust from sputa of tuberculous patients. Jpn J Microbiol 18:271–277
    [Google Scholar]
  35. Tsukamura M., Van der Meulen H. J., Grabow W. O. H. 1983; Numerical taxonomy of rapidly growing, scotochromogenic mycobacteria of the Mycobacterium parafortuitum complex: Mycobacterium austroafricanum sp. nov. and Mycobacterium diernhoferi sp. nov. nom. rev. Int J Syst Bacteriol 33:460–469
    [Google Scholar]
  36. Vaneechoutte M., de Beenhouwer H., Claeys G., Verschraegen G., de Rouck A., Paepe N., Elaichouni A., Portaels F. 1993; Identification of Mycobacterium species by using amplified ribosomal DNA restriction analysis. J Clin Microbiol 8:2061–2065
    [Google Scholar]
  37. Wayne L. G., Krasnow I. 1966; Preparation of tuberculosis susceptibility testing mediums by impregnated discs. Am J Pathol 45:769–771
    [Google Scholar]
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