The taxonomic status of Arthrobacter sp. Y-11T, which was described as a squalene-degrading bacterium, was investigated by chemotaxonomic and genetic methods. The strain possesses wall chemotype IV, MK-9(H2) as the predominant menaquinone, mycolic acids, and straight-chain, saturated and monounsaturated fatty acids, with considerable amounts of tuberculostearic acid. The DNA G+C content is 67·5 mol%. 16S rRNA gene sequence analysis and quantitative DNA-DNA hybridization experiments provided strong evidence that strain Y-11Trepresents a new species within the genus Corynebacterium, for which the name Corynebacterium terpenotabidum sp. nov. is proposed. The type strain of C. terpenotabidum is strain Y-11T(= IFO 14764T).
BendingerB.,
KroppenstedtR. M.,
KlatteS.,
AltendorfK.1992; Chemotaxonomic differentiation of coryneform bacteria isolated from biofilters. Int J Syst Bacteriol 42:474–486
CollinsM. D.1987a; Transfer of Arthrobacter variabilis (Müller) to the genus Corynebacterium, as Corynebacterium variabilis comb. nov. Int J Syst Bacteriol 37:287–288
CollinsM. D.1987b; Transfer of Brevibacterium ammoniagenes (Cooke and Keith) to the genus Corynebacterium as Corynebacterium ammoniagenes comb. nov. Int J Syst Bacteriol 37:442–443
CollinsM. D.,
CumminsC. S.1986; Genus Corynebacterium
. In Bergey’s Manual of Systematic Bacteriology21266–1283SneathP. H. A.,
MairN. S.,
SharpeM. E.,
HoltJ. G.. Baltimore: Williams & Wilkins;
CollinsM. D.,
GoodfellowM.,
MinnikinD. E.1982; Fatty acid composition of some mycolic acid-containing coryneform bacteria. J Gen Microbiol 128:2503–2509
CollinsM. D.,
BurtonR. A.,
JonesD.1988; Corynebacterium amycolatum sp. nov., a new mycolic acid-less Corynebacterium species from human skin. FEMS Microbiol Lett 49:349–352
CollinsM. D.,
ShimadaJ.,
StackebrandtE.1989; Phylogenetic evidence for the transfer of Caseobacter polymorphys (Crombach) to the genus Corynebacterium
. Int J Syst Bacteriol 39:7–9
EzakiT.,
HashimotoY.,
YabuuchiE.1989; Fluorometric deoxyribonucleic acid–deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strain. Int J Syst Bacteriol 39:224–229
FunkeG.,
LawsonP. A.,
CollinsM. D.1995; Heterogeneity within human-derived Centers for Disease Control and Prevention (CDC) coryneform group ANF-1-like bacteria and description of Corynebacterium auris sp. nov. Int J Syst Bacteriol 45:735–739
Herrera-AlcarazE. A.,
Valero-GuillénP. L.,
Martin-LuengoF.,
SorianoF.1990; Taxonomic implications of the chemical analysis of the D2 group of corynebacteria. FEMS Microbiol Lett 72:341–344
HiraishiA.1992; Direct automated sequencing of 16S rRNA amplified by polymerase chain reaction from bacterial culture without DNA purification. Lett Appl Microbiol 15:210–213
IkeguchiN.,
NihiraT.,
KishimotoA.,
YamadaT.1988; Oxidative pathway form squalene to geranylacetone in Arthrobacter sp. strain Y-ll. Appl Environ Microbiol 54:381–385
KimuraM.1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120
LaneelleM. A.,
AsselineauJ.,
WelbyM.,
NorgardM. V.,
ImaedaT.,
PolliceM. C.,
BarksdaleL.1980; Biological and chemical bases for the reclassification of Brevibacterium vita-rumen (Bechdel et al.) Breed (Approved Lists, 1980) as Corynebacterium vitarumen (Bechdel et al.) comb, nov and Brevibacterium liquefaciens Okabayashi and Masuo (Approved Lists, 1980) as Corynebacterium liquefaciens (Okabayashi and Masuo) comb. nov. Int J Syst Bacteriol 30:539–546
LechevalierM. P.,
LechevalierH. A.1970; Chemical composition as a criterion in the classification of aerobic actinomycetes. Int J Syst Bacteriol 20:435–443
MesbahM.,
PremachandranU.,
WhitmanW. B.1989; Precise measurement of the G + C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167
MinnikinD. E.,
AlshamaonyL.,
GoodfellowM.1975; Differentiation of Mycobacterium, Nocardia and related taxa by thin-layer Chromatographie analysis of whole-organism methanolysates. J Gen Microbiol 88:200–206
MinnikinD. E.,
CollinsM. D.,
GoodfellowM.1979; Fatty acid and polar lipid composition in the classification of Cellulomonas, Oerskovia and related taxa. J Appl Bacteriol 47:87–95
Pascual C,
LawsonP. A.,
FarrowJ. A.,
GimenezM. N.,
CollinsM. D.1995; Phylogenetic analysis of the genus Corynebacterium based on 16S rRNA gene sequences. Int J Syst Bacteriol 45:724–728
PitcherD. G.1983; Deoxyribonucleic acid base composition of Corynebacterium diphtheriae and other corynebacteria with cell wall type IV. FEMS Microbiol Lett 16:291–295
RiegelP., , de BrielD.,
PrevostG.,
JehlF.,
MonteilH.1993a; Proposal of Corynebacterium propinquum sp. nov. for Corynebacterium group ANF-3 strains. FEMS Microbiol Lett 113:229–234
RiegelP., , de BrielD.,
ProvostG.,
JehlF.,
MonteilH.,
MinckR.1993b; Taxonomic study of Corynebacterium group ANF-1 strains: proposal of Corynebacterium afermentans sp. nov. containing the subspecies C. afermentans subsp. afermentans subsp. nov. and C. afermentans subsp. lipophilum subsp. nov. Int J Syst Bacteriol 43:287–292
RuimyR.,
RiegelP.,
BoironP.,
MonteilH.,
ChristenR.1995; Phylogeny of the genus Corynebacterium deduced from analyses of small-subunit ribosomal DNA sequences. Int J Syst Bacteriol 45:740–746
TamaokaJ.,
KomagataK.1984; Determination of DNA base composition by reversed-phase high performance liquid chromatography. FEMS Microbiol Lett 25:125–128
WilletJ. D.,
SharplessK. B.,
LordK. E., , van TamelenE. E.,
ClaytonP. B.1967; Squalene-2,3-oxide, and intermediate in enzymatic conversion of squalene to lanosterol and cholesterol. J Biol Chem 242:4182–4191