Strain 130ZT was isolated from the bovine rumen. It is a facultatively anaerobic, pleomorphic, Gram-negative rod. It exhibits a ‘Morse code’ form of morphology, which is characteristic of the genus Actinobacillus. Strain 130ZT is a capnophilic, osmotolerant succinogen that utilizes a broad range of sugars. It accumulates high concentrations of succinic acid (> 70gl-1). Strain 130ZT is positive for catalase, oxidase, alkaline phosphatase and β-galactosidase, but does not produce indole or urease. Acid but no gas is produced from d-glucose and d-fructose. 16S rRNA sequence analysis places strain 130ZT within the family Pasteurellaceae; the most closely related members of the family Pasteurellaceae have 16S rRNA similarities of 95.5% or less with strain 130ZT. Strain 130ZTwas compared with Actinobacillus lignieresii and the related Bisgaard Taxa 6 and 10. Based upon morphological and biochemical properties, strain 130ZT is most similar to members of the genus Actinobacillus within the family Pasteurellaceae. It is proposed that strain 130ZT be classified as a new species, Actinobacillus succinogenes. The type strain of Actinobacillus succinogenes sp. nov. is ATCC 55618T.
BisgaardM.,
MuttersR.,
MannheimW.1983; Characterization of some previously unreported taxa isolated from guineapigs (Cavia porcellus) and provisionally classed with the ‘HPA-group’. INSERM 114:227–244
BisgaardM.,
PhillipsJ. E.,
MannheimW.1986; Characterization and identification of bovine and ovine Pasteurellaceae isolated from the oral cavity and rumen of apparently normal cattle and sheep. Acta Pathol Microbiol Immunol Scand Sect B Microbiol 94:9–17
DewhirstF. E.,
PasterB. J.,
OlsenI.,
FraserG. J.1993; Phylogeny of the Pasteurellaceae as determined by comparison of 16S ribosomal ribonucleic acid sequences. Zentbl Bakteriol 279:35–44
FosterG.,
RossH. M.,
MalnickH.,
WillemsA.,
GarciaP.1996; Actinobacillus delphinicola sp. nov., a new member of the family Pasteur ellaceae Pohl (1979) 1981 isolated from sea mammals. Int J Syst Bacteriol 46:648–652
GuettlerM. V.,
JainM. K.,
SoniB. K.1996aProcess for making succinic acid, microorganisms for use in the process and methods of obtaining the microorganisms. US Patent 5 504004
GuettlerM. V.,
JainM. K.,
RumlerD.1996bMethod for making succinic acid, bacterial variants for use in the process, and methods for obtaining the variants. US Patent 5573931
HollanderR.,
Hess-ReihseA.,
MannheimW.1981; Respiratory quinones in Haemophilus, Pasteurella and Actinobacillus: pattern, function, and taxonomic evaluation. In Haemophilus, Pasteurella and Actinobacillus92–93KilianM.,
FrederiksenW.,
BibersteinE. L.
London: Academic Press;
JantzenE.,
BerdalB. P.,
OmlandT.1981; Cellular and fatty acid taxonomy of Haemophilus, Pasteurella, and Actinobacillus
. In Haemophilus, Pasteurella and Actinobacillus197–203KilianM.,
FrederiksenW.,
BibersteinE. L.
London: Academic Press;
KilianM.,
BibersteinE. L.1984; Genus II Haemophilus
. In Bergey’s Manual of Systematic Bacteriology1561–562KriegN. R.,
HoltJ. G.
Baltimore: Williams & Wilkins;
KusukawaN.,
UemoriT.,
AsadaK.,
KatoI.1990; Rapid and reliable protocol for direct sequencing of material amplified by the polymerase chain reaction. Biotechniques 9:66–72
MannheimW.1984; Family III Pasteur ellaceae Pohl 1981a, 382. In Bergey’s Manual of Systematic Bacteriology1550–552KriegN. R.,
HoltJ. G.
Baltimore: Williams & Wilkins;
MendesE. N.,
QueirozD. M. M.,
DewhirstF. E.,
PasterB. J.,
MouraS. B.,
FoxJ. G.1996; Helicobacter trogontum sp. nov., isolated from the rat intestine. Int J Syst Bacteriol 46:916–921
MesbahM.,
PremachandranU.,
WhitmanW. B.1989; Precise measurement of the G + C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167
PhillipsJ. E.1984; Genus III Actinobacillus Brumpt 1910, 849AL
. In Bergey’s Manual of Systematic Bacteriology1570–575KriegN. R.,
HoltJ. G.
Baltimore: Williams & Wilkins;
PhillipsJ. E.1992; The genus Actinobacillus
. In The Prokaryotes, a Handbook on the Biology of Bacteria: Eco-physiology, Isolation, Identification, Applications, 2. IV:3342–3351BalowsA.,
TrüperH. G.,
DworkinM.,
HarderW.,
SchleiferK.-H.
New York: Springer;
SamitzE. M.,
BibersteinE. L.1991; Actinobacillus suis-like organisms and evidence of hemolytic strains of Actinobacillus lignieresii in horses. Am J Vet Res 52:1245–1251
StackebrandtE.,
GoebelB. M.1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849
Van der WerfM. J.,
GuettlerM. V.,
JainM. K.,
ZeikusJ. G.1997; Environmental and physiological factors affecting the succinate product ratio during carbohydrate fermentation by Actinobacillus sp. 130Z
. Arch Microbiol 167:332–342
WardA. C. S.,
JaworskiM. D.,
EddowJ. M.,
CorbeilL. B.1995; A comparative study of bovine and ovine Haemophilus somnus isolates. Can J Vet Res 59:173–178
YokoyamaM. T.,
JohnsonK. A.1988; Microbiology of the rumen and intestine. In The Ruminant Animal: Digestive Physiology and Nutrition125–144ChurchD. C.
NJ; Prentice Hall: