1887

Abstract

Lindane-degrading activity under aerobic conditions has been observed in two bacterial strains: UT26, phenotypically identified as , and a new single unidentified isolate named RP55577. The (16S rDNA) sequences for both strains and the phenotypic characteristics for the unidentified isolate RP5557were determined. RP5557does not have high identity (less than 90% in all cases) with any sequence in the GenBank or RDP databases. A phylogenetic analysis based on sequences indicated that RP5557belongs to the - in a coherent phylum that includes the genera and (100% bootstrap), whereas UT26 is clearly separate from the cluster. Based on the phylogenetic analyses and on the phenotypic characteristics, a new genus, , containing a single species, , is proposed for strain RP5557(= LMG 18385), which becomes the type strain.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-49-1-19
1999-01-01
2022-08-13
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/49/1/ijs-49-1-19.html?itemId=/content/journal/ijsem/10.1099/00207713-49-1-19&mimeType=html&fmt=ahah

References

  1. Altshul S. F., Gish W., Miller W., Myers E. W., Lipman D. J. 1990; Basic local alignment search tool. J Mol Biol 215:403–410
    [Google Scholar]
  2. Bhuyan S., Sahu S. K., Adhya T. K., Sethunathan N. 1992; Accelerated aerobic degradation of γ-hexachlorocyclohexane in suspensions of flooded and non flooded soils pretreated with hexachlorocyclohexane. Biol Fertil Soils 12:279–284
    [Google Scholar]
  3. Brenner D. J., McWorter A., Knuston J. K. L., Steigerwalt A. G. 1982; Escherichia vulneris: a new species of Enter obacteriaceae associated with human wounds. J Clin Microbiol 15:1133–1140
    [Google Scholar]
  4. Felsenstein J. 1985; Confidence limit on phylogenies: an approach using the bootstrap. Evolution 39:783–791
    [Google Scholar]
  5. Fitch W. M. 1971; Toward defining the course of evolution: minimum change for the specific tree topology. Syst Zool 20:406–416
    [Google Scholar]
  6. Galtier N., Gouy M., Gautier C. 1996; Seaview and phylo win : two graphic tools for sequence alignment and molecular phylogeny. Cabios 12:543–548
    [Google Scholar]
  7. Jagnow G., Haider K., Ellwardt P. C. 1977; Anaerobic dechlorination and degradation of hexachlorocyclohexane iso-mers by anaerobic and facultative anaerobic bacteria. Arch Microbiol 115:285–292
    [Google Scholar]
  8. Kimura M. 1983 The Neutral Theory of Molecular Evolution Cambridge: Cambridge University Press;
    [Google Scholar]
  9. MacRae I. C, Raghu K., Bautista E. M. 1969; Anaerobic degradation of the insecticide Lindane by Clostridium sp. Nature 221:859–860
    [Google Scholar]
  10. Maidak B. L., Olsen G. J., Larsen N., Overbeek R., McCaughey M. J., Woese C. R. 1997; The RDP (Ribosomal Database Project). Nucleic Acids Res 25:109–111
    [Google Scholar]
  11. Normand P., Orso S., Cournoyer B., Jeannin P., Chapelon G., Dawson J., Evtushenko L., Misra A. 1996; Molecular phylogeny of the genus Frankia and related genera and emendation of the family Frankiaceae. Int J Syst Bacteriol 46:1–9
    [Google Scholar]
  12. Ohisa N., Yamaguchi M., Kuriara N. 1980; Lindane degradation by cell-free extracts of Clostridium rectum. Arch Microbiol 125:221–225
    [Google Scholar]
  13. Olsen G. J., Matsuda H., Hagstrom R., Overbeek R. 1994; fastDNAml : a tool for construction of phylogenetic trees of DNA sequences using maximum likelihood. Comput Appl Biosci 10:41–48
    [Google Scholar]
  14. Peyret M., Freney J., Meugnier H., Fleurette J. 1989; Determination of G + C content of DNA using HPLC for identification of staphylococci and micrococci. Res Microbiol 140:467–475
    [Google Scholar]
  15. Saitou N., Nei M. 1987; The neighbor-joining method : a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  16. Senoo K., Wada H. 1989; Isolation and identification of an aerobic y-HCH-decomposing bacterium from soil. Soil Sci Plant Nutr 35:79–87
    [Google Scholar]
  17. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849
    [Google Scholar]
  18. Thomas J. C., Berger F., Jacquier M., Bernillon D., Baud-Grasset F., Truffaut N., Normand P., Vogel T. M., Simonet P. 1996; Isolation and characterization of a novel γ-hexachlorohexane-degrading bacterium. J Bacteriol 178:6049–6055
    [Google Scholar]
  19. Thompson J. D., Higgins D. G., Gibson T. J. 1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, positions-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680
    [Google Scholar]
  20. Voerman S., Besemer A. F. H. 1970; Residues of dieldrin, lindane, DDT and parathion in light sandy soil after repeated application throughout a period of 15 years. J Agric Food Chem 35:71–77
    [Google Scholar]
  21. Vogel T. M., Criddle C. S., McCarty P. L. 1987; Transformation of halogenated aliphatic compounds. Environ Sci Technol 21:722–736
    [Google Scholar]
  22. Wada E. W., Peel D. A. 1989; Rapid degradation of γ-HCH in upland soil after multiple applications. Soil Sci Plant Nutr 35:71–77
    [Google Scholar]
  23. Wayne L. G., Brenner D. J., Colwell R. R. 9 other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-49-1-19
Loading
/content/journal/ijsem/10.1099/00207713-49-1-19
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error