1887

Abstract

The group comprises the four valid species and Some isolates of . are known to be psychrotolerant (growth at 7 °C or below). Here, specific sequence differences are described between the 16S rDNA, the 23S rDNA, the 16S–23S rDNA spacer region and the genes of the major cold-shock protein homologue in a variety of psychrotolerant and mesophilic . and . strains. Randomly amplified polymorphic DNA analysis using three different primers clearly separated psychrotolerant strains of both species from the rest of the . group, as did inverse PCR patterns of the rDNA operons. These data strongly support a hitherto unrecognized fifth sub-group within the . species group comprising psychrotolerant, but not mesophilic, . strains. Despite the latter finding, the DNA sequences investigated exhibited a high degree of sequence similarity indicating a close relationship between the species of the . group. Considering the unusual importance of . in both food poisoning and food spoilage and to avoid merging all species of the group, a new species, sp. nov., comprising psychrotolerant ‘’ strains, is proposed. Isolates of the new species grow at 4–7°C but not at 43°C and can be identified rapidly using rDNA or targeted PCR. The type strain is . WSBC 10204(= DSM 11821).

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1998-10-01
2022-08-13
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