gen. nov., with descriptions of sp. nov., isolated from kusaya gravy, and transfer of to comb. nov. Free

Abstract

Two strains of helical, halophilic, Gram-negative, heterotrophic bacteria were isolated from kusaya gravy which is a traditional Japanese fermented brine. These strains were motile by means of a single polar or bipolar tuft flagellum. They had a large cell size, were helical, formed coccoid bodies, were microaerophilic and had quinone type Q-8. The DNA G+C content of the strains was 44–45 mol%. A detailed investigation of the phenotypic, chemotaxonomic and phylogenetic characteristics of the strains revealed that they represent a new species of halophilic helical bacteria. The sequence of the 16S rRNA gene of strain H7, designated the type strain of the new isolates, and all of the species except for were determined. Phylogenetic analysis indicated that these strains were closely related to , with enough distance to separate the /new isolate H7cluster from on the genus level. It is proposed that a new genus, , be created; this genus should include ATCC 19193(formerly ) as the type species, as well as JCM 10129(=H7).

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1998-10-01
2024-03-29
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References

  1. Altschul S. A., Gish W., Miller W., Myers E. W., Lipman D. J. 1990; Basic local alignment search tool. J Mol Biol 215:403–410
    [Google Scholar]
  2. Baumann L., Baumann P., Mandel M., Allen R. D. 1972; Taxonomy of aerobic marine eubacteria. J Bacteriol 110:402–29
    [Google Scholar]
  3. Burdon K. L. 1946; Fatty material in bacteria and fungi revealed by staining dried, fixed slide preparations. J Bacteriol 52:665–678
    [Google Scholar]
  4. Conn H. J., Bartholomew J. W., Jennison M. W. 1957; Staining methods. In Manual of Microbiological Methods10–36 Edited by Society of American Bacteriologists New York: McGraw-Hill;
    [Google Scholar]
  5. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229
    [Google Scholar]
  6. Fujii T. 1977; Studies on 'Kusaya'-I. Comparison of composition of ‘Kusaya’ brine at Niijima and Oshima islands. Nippon Suisan Gakkaishi 43:517–521
    [Google Scholar]
  7. Fujii T. 1978; Microbial flora of ‘Kusaya’ brine of Niijima and Oshima islands. Nippon Suisan Gakkaishi 44:45–48
    [Google Scholar]
  8. Fujii T., Sugita H., Deguchi Y. 1985; Obligately anaerobic bacteria isolated from ‘Kusaya’ gravy. Nippon Suisan Gakkaishi 51:473–478
    [Google Scholar]
  9. Fujii T., Hayashi M., Okuzumi M. 1990; New device for isolation of spiral bacteria from ‘Kusaya’ gravy. Nippon Suisan Gakkaishi 56:1661
    [Google Scholar]
  10. Fujii T., Kuda T., Okuzumi M. 1993; Anaerobic microflora of ‘Kusaya’ gravy. Nippon Suisan Gakkaishi 59:185
    [Google Scholar]
  11. Hamana K., Sakane T., Yokota A. 1994; Polyamine analysis of the genera Aquaspirillum Magnetospirillum Oceanospirillum and Spirillum. J Gen Appl Microbiol 40:75–82
    [Google Scholar]
  12. Holt J. G., Krieg N. R., Sneath P. H. A., Staley J. T., Williams S. T. 1994 Bergey’s Manual of Determinative Bacteriology, 9. Baltimore: Williams Wilkins;
    [Google Scholar]
  13. Hylemon P. B., Wells J. S. Jr, Krieg N. R., Jannasch H. W. 1973; The genus Spirillum a taxonomic study. Int J Syst Bacteriol 23:340–380
    [Google Scholar]
  14. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Biol 16:111–120
    [Google Scholar]
  15. Kosuge T., Yokota M., Ohba H., Nagano H. 1978; Study on kusaya-I. Antiseptic effects of kusaya brine. Nippon Suisan Gakkaishi 44:1141–1146
    [Google Scholar]
  16. Krieg N. R. 1984; Aerobic/microaerophilic, motile, helical/ vibrioid Gram-negative bacteria. In Bergefs Manual of Systematic Bacteriology 171–95 Krieg N. R., Holt J. G. Baltimore: Williams Wilkins;
    [Google Scholar]
  17. Linn D. M., Krieg N. R. 1978; Occurrence of two organisms in cultures of the type strain of Spirillum lunatum proposal for rejection of the name Spirillum lunatum and characterization of Oceanospirillum marts subsp. williamsae and an unclassified vibrioid bacterium. Int J Syst Bacteriol 28:132–138
    [Google Scholar]
  18. Pot B., Gillis M., Hoste B., Van De Velde A., Bekaert F., Kersters K., De Ley J. 1989; Intra- and intergenetic relationships of the genus Oceanospirillum. Int J Syst Bacteriol 39:23–34
    [Google Scholar]
  19. Pot B., Gillis M., De Ley J. 1992; The genus Oceanospirillum. In The Prokaryotes A Handbook on the Biology of Bacteria. Ecophysiology, Isolation, Identification, Applications, 2. 43230–3236 Balows A., Trüper H. G., Dworkin M., Harder W., Schleifer K.-H. New York: Springer-Verlag;
    [Google Scholar]
  20. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4406–425
    [Google Scholar]
  21. Sakane T., Yokota A. 1994; Chemotaxonomic investigation of heterotrophic, aerobic and microaerophilic spirilla, the genera Aquaspirillum Magnetospirillum and Oceanospirillum. Syst Appl Microbiol 17:128–134
    [Google Scholar]
  22. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: a Laboratory Manual, 2. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  23. Satomi M., Kimura B., Mizoi M., Satou T., Fujii T. 1997a; Tetragenococcus muriaticus sp. nov., a new moderately halo-philic lactic acid bacterium isolated from fermented squid liver sauce. Int J Syst Bacteriol 47:832–836
    [Google Scholar]
  24. Satomi M., Kimura B., Takahashi G., Fujii T. 1997b; Microbial diversity of kusaya gravy. Fish Sci (Tokyo) 63:1019–1023
    [Google Scholar]
  25. Shimidu A., Mochizuki A., Shimizu U., Aiso K. 1967; Studies of kusaya-I. Chemical composition and preserving effect of the curing brine for kusaya. Nippon Suisan Gakkaishi 33:1143–1146
    [Google Scholar]
  26. Shimidu U., Aiso K., Shimidu W., Mochizuki A. 1969; Studies of kusaya-II. Microbiological examination of a kusaya brine. Nippon Suisan Gakkaishi 35:109–115
    [Google Scholar]
  27. Stackebrandt E., Murray R. G. E., Truper H. G. 1988; Proteo-bacteria classis nov., a name for the phylogenetic taxon that includes the “ Purple bacteria and their relatives”. Int J Syst Bacteriol 38:321–325
    [Google Scholar]
  28. Suzuki T., Yamasato K. 1994; Phylogeny of spore-forming lactic acid bacteria based on 16S rRNA gene sequences. FEMS Microbiol Lett 115:13–18
    [Google Scholar]
  29. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reversed-phase high performance liquid chromatography. FEMS Microbiol Lett 25:125–128
    [Google Scholar]
  30. Terasaki Y. 1972; Studies on the genus Spirillum Ehrenberg. I. Morphological, physiological, and biochemical characteristics of water spirilla. Bull Suzugamine Women’s Coll Nat Sci 16:1–46
    [Google Scholar]
  31. Terasaki Y. 1973; Studies on the genus Spirillum Ehrenberg. II. Comments on type and reference strains of Spirillum and description of new species and new subspecies. Bull Suzugamine Women’s Coll Nat Sci 17:1–71
    [Google Scholar]
  32. Terasaki Y. 1975; Freeze-dried cultures of water spirilla made on experimental basis. Bull Suzugamine Women’s Coll Nat Sci 19:1–10
    [Google Scholar]
  33. Terasaki Y. 1979; Transfer of five species and two subspecies of Spirillum to other genera (Aquaspirillum and Oceanospirillum), with emended descriptions of the species and subspecies. Int J Syst Bacteriol 29:130–144
    [Google Scholar]
  34. Thompson J. D., Higgins D. G., Gibson T. J. 1994; CLUSTAL w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680
    [Google Scholar]
  35. Watanabe N. 1959; On four new halophilic species of Spirillum. Bot Mag Tokyo 72:77–86
    [Google Scholar]
  36. Wayne L. G., Brenner D. J., Colwell R. R. 9 other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464
    [Google Scholar]
  37. Weisburg W. G., Barns S. M., Pelletier D. A., Lane D. J. 1991; 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 173:697–703
    [Google Scholar]
  38. Williams M. A., Rittenberg S. C. 1957; A taxonomic study of the genus Spirillum Ehrenberg. Int Bull Bacteriol Nomencl Taxon 7:49–111
    [Google Scholar]
  39. Woese C. R., Weisburg W. G., Hahn C. M., Paster B. J., Zablen L. B., Lewis B. J., Macke T. J., Ludwig W., Stackebrandt E. 1985; The phylogeny of purple bacteria: the gamma subdivision. Syst Appl Microbiol 6:25–33
    [Google Scholar]
  40. Yamada Y., Kuraishi I. 1982; Ubiquinone and Menaquinone. In Biseibutsu no Kagakubunrui Jikkenhou143–155 Komagata K. Tokyo: Gakkai Shuppan Center (in Japanese);
    [Google Scholar]
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