Sequences of the RNase P RNA gene were investigated for the phylogenetic analysis of the genus . Aligned nucleotide sequences, determined from the PCR-amplified RNase P RNA gene of representative strains of the genus , displayed 94·2±1·3% interspecific variances. The intraspecific similarity value was 99·7−100% in all species tested. K161and ‘’ K76displayed identical RNase P RNA gene sequences in the region that was determined and sp. K180 showed sequences distinct from validly described species with a similarity value of 94·6±10%. The phylogenetic trees constructed by aligning the sequences either within the genus or with other Gram-positive bacteria were similar to the ones derived using sequences of the 16S rDNA gene. Advantageous features of this gene for application as a molecular phyletic marker are discussed.


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