1887

Abstract

An actinomycete strain, ‘’ JCM 3332, was found to have properties consistent with its in the genus . An almost complete gene sequence of the 16S rDNA of the strain was determined following cloning and sequencing of the amplified gene. The sequence was aligned with those available for nocardiae and phylogenetic trees were inferred using four tree-making algorithms. The organisms consistently formed a distinct clade with the type strain of . However, DNA relatedness experiments showed that the strain and DSM 43397belonged to two distinct genomic species. The organism was also distinguished from representatives of all of the validly described species of using a combination of phenotypic properties. These genotypic and phenotypic data show that the strain merits recognition as a new species of the genus . The name proposed for the new species is sp. nov. The type strain is JCM 3332.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-48-3-901
1998-07-01
2024-04-18
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/48/3/ijs-48-3-901.html?itemId=/content/journal/ijsem/10.1099/00207713-48-3-901&mimeType=html&fmt=ahah

References

  1. Boiron P., Provost F., Duport B. 1993 Laboratory Methods for the Diagnosis of Nocardiosis Paris: Institut Pasteur;
    [Google Scholar]
  2. Chun J. 1995 Computer-assisted classification and identification of actinomycetes PhD thesis, University of Newcastle upon Tyne
    [Google Scholar]
  3. Chun J., Goodfellow M. 1995; A phylogenetic analysis of the genus Nocardia with 16S rRNA gene sequences. Int J Syst Bacteriol 45:240–245
    [Google Scholar]
  4. Chun J., Kang S.-O., Hah Y. C., Goodfellow M. 1996; Phylogeny of mycolic acid-containing actinomycetes. J Ind Microbiol 17:205–213
    [Google Scholar]
  5. Chun J., Blackall L. L., Kang S.-O., Hah Y. C., Goodfellow M. 1997; A proposal to reclassify Nocardia pinensis Blackall et al. as Skermania piniformis gen. nov., comb. nov. Int J Syst Bacteriol 47:127–131
    [Google Scholar]
  6. Collins M. D., Goodfellow M., Minnikin D. E. 1982; A survey of the structures of mycolic acid in Corynebacterium and related taxa. J Gen Microbiol 128:129–149
    [Google Scholar]
  7. Felsenstein J. 1981; Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376
    [Google Scholar]
  8. Felsenstein J. 1985; Confidence limits on phytogenies: an approach using the bootstrap. Evolution 39:783–791
    [Google Scholar]
  9. Felsenstein J. 1993; phylip (phylogenetic inference package), version 3.5c. Department of Genetics, University of Washington; Seattle, USA:
    [Google Scholar]
  10. Fitch W. M. 1972; Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416
    [Google Scholar]
  11. Fitch W. M., Margoliash E. 1967; Construction of phylogenetic trees: a method based on mutation distances as estimated from cytochrome c sequences is of general applicability. Science 155:279–284
    [Google Scholar]
  12. Goodfellow M. 1992; The family Nocardiaceae. In The Prokaryotes, 2nd edn pp. 1188–1213 Edited by Balows A, Truper H. G., Dworkin M., Harder W., Schleifer K.-H. New York: Springer;
    [Google Scholar]
  13. Goodfellow M. 1998; The actinomycetes: Nocardia and related genera. In Topley and Wilson’s Microbiology and Microbial Infections, 9th edn vol. 2 Systematic Bacteriology Edited by Balows A., Duerden B. I. London: Edward Arnold; (in press)
    [Google Scholar]
  14. Goodfellow M., Lechevalier M. P. 1989; Genus Nocardia Trevisan 1889, 9AL. In Bergey s Manual of Systematic Bacteriology pp. 2350–2361 Edited by Williams S. T., Sharpe M. E., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  15. Gordon R. E., Barnett D. A., Handerhan J. E., Pang C. H. N. 1974; Nocardia coeliaca, Nocardia autotrophica, and the Nocardin strain. Int J Syst Bacteriol 24:54–63
    [Google Scholar]
  16. Jones K. L. 1949; Fresh isolates of actinomycetes in which the presence of sporogenous aerial mycelia is a fluctuating characteristic. J Bacteriol 57:141–145
    [Google Scholar]
  17. Jukes T. H., Cantor C. R. 1969; Evolution of protein molecules. In Mammalian Protein Metabolism pp. 21132 Edited by Munro H. N. New York: Academic Press;
    [Google Scholar]
  18. Kafatos F. C., Jones C. W., Efsratiadis A. 1979; Determination of nucleic acid sequence homologies and relative concentrations by a dot hybridization procedure. Nucleic Acids Resl1541–1545
    [Google Scholar]
  19. Koser S. A. 1924; Correlation of citrate utilization by members of the colon-aerogenes groups with other differential characteristics and with habitat. J Bacteriol 9:59–77
    [Google Scholar]
  20. Kudo T., Hatai K., Seino A. 1988; Nocardia seriolae sp. nov. causing nocardiosis of cultured fish. Int J Syst Bacteriol 38:173–178
    [Google Scholar]
  21. Lechevalier M. P. 1976; The taxonomy of the genus Nocardia: some light at the end of tunnel?. In The Biology of the Nocar diae pp. 138 Edited by Goodfellow M., Brownell G. H., Serrano J. A. London: Academic Press;
    [Google Scholar]
  22. Lechevalier M. P., Lechevalier H. A. 1970; Chemical composition as a criterion in the classification of aerobic actino- mycetes. Int J Syst Bacteriol 20:435–443
    [Google Scholar]
  23. Liu Z., Ruan J. S., Yan X. C. 1983; The new species of Nocardia. Acta Microbiol Sinica 23298–304 (in Chinese)
    [Google Scholar]
  24. Mandel M., Marmur J. 1968; Use of ultraviolet absorbance temperature profile for determining the guanine plus cytosine content of DNA. Methods Enzymol 12B:195–206
    [Google Scholar]
  25. Minnikin D. E., Hutchinson I. G., Caldicott A. B., Goodfellow M. 1980; Thin-layer chromatography of methanolysates of mycolic acid-containing bacteria. J Chromatogr 188:221–233
    [Google Scholar]
  26. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M„, Schaal A., Parlett J. H. 1984; An integrated procedure for the extraction of isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241
    [Google Scholar]
  27. Modarska H., Mordarski M., Goodfellow M. 1972; Chemo- taxonomic characters and classification of some nocardioform bacteria. J Gen Microbiol 71:77–86
    [Google Scholar]
  28. Ruimy R., Riegel P., Carlotti A., Boiron P., Bernardin G., Monteil H., Wallace R. J. Jr, Christen R. 1996; Nocardia pseudobrasiliensis sp. nov., a new species of Nocardia which groups bacterial strains previously identified as Nocardia brasiliensis and associated with invasive diseases. Int J Syst Bacteriol 46:259–264
    [Google Scholar]
  29. Saddler G. S., Tavecchia P., Lociuro S., Zanol M., Colombo L., Selva E. 1991; Analysis of madurose and other actinomycete whole cell sugars by gas chromatography. J Microbiol Methods 14:185–191
    [Google Scholar]
  30. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for constructing phylogenetic trees. Mol Biol Evol 4406–425
    [Google Scholar]
  31. Sasser M. 1990 Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids. Technical Note 101 Newark, DE: Microbial ID;
    [Google Scholar]
  32. Staneck J. L., Roberts G. D. 1974; Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 28:226–231
    [Google Scholar]
  33. Trevisan V. 1889 Generiele Specie delle Batteriacee. Milan: Zanaboni & Gabuzzi;
    [Google Scholar]
  34. Yano I., Imaeda T., Tsukamura M. 1990; Characterization of Nocardia nova. Int J Syst Bacteriol 40:170–174
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-48-3-901
Loading
/content/journal/ijsem/10.1099/00207713-48-3-901
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error