1887

Abstract

A novel, obligately anaerobic bacterium capable of hydrolysing lipids was isolated from a tropical anaerobic lagoon receiving waste water from an edible oil mill. The isolate had many characteristics similar to those of members of the genus The isolate showed lipolytic activity on tributyrin, triolein and groundnut oil in qualitative plate clearance assays, which has not been reported for the type strain of the genus It did not require n-valerate supplementation for growth on glucose. Acetate and propionate were the only volatile fatty acids produced from glucose fermentation with propionate as the major end product. The isolate could grow optimally at pH 6.8 and at a temperature of 40 °C. It could tolerate NaCI concentrations of up to 40 g l. The G+C content of the DNA was 40 mol% as determined by thermal denaturation analysis. Comparison of partial 16S rRNA gene sequences revealed that the isolate was most closely related to genus with 91% sequence similarity (250 bp compared) to strain GA 192. On the basis of the results obtained in the present investigation, it is suggested that a new species of should be created for this novel isolate and the name is proposed for this new species. The type strain is strain CF1B(= MCMB 505).

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1998-07-01
2022-12-06
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References

  1. Ausubel F. M., Brent R., Kingston R. E., Moore D. D., Seidman J. G., Smith J. A., Struhl K. 1995 Current Protocols in Molecular Biology New York: Wiley;
    [Google Scholar]
  2. Birnboim H. C., Doly J. 1979; A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res 7:1513–1523
    [Google Scholar]
  3. Cato E. P., George W. L., Finegold S. M. 1986; Genus Clostridium Prazmowski 1880, 23AL. In Bergeys Manual of Systematic Bacteriology vol. 2 pp. 11411200 Edited by Sneath P. H. A., Mair N. S., Sharpe M. E., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  4. Haikara A. 1992; The genera Pectinatus and Megasphaera. In The Prokaryotes vol. 2 pp. 1993–2004 Edited by Balows A, Truper H. G., Dworkin M., Harder W., Schleifer K.-H. New York: Springer;
    [Google Scholar]
  5. Henderson C. 1971; A study of the lipase produced by Anaerovibrio lipolytica, a rumen bacterium. J Gen Microbiol 65:81–89
    [Google Scholar]
  6. Hespell R. B., Paster B. J., Dewhirst F. E. 1992; The genus Selenomonas. In The Prokaryotes vol. II pp. 2005–2013 Edited by Balows A, Truper H. G., Dworkin M., Harder W., Schleifer K.-H. New York: Springer;
    [Google Scholar]
  7. Higgins D. G., Bleasby A. J., Fuchs R. 1992; clustal v, improved software for multiple sequence alignment. Comput Appl BiosciS189–191
    [Google Scholar]
  8. Hobson P. N., Mann S. O. 1961; The isolation of glycerol fermenting and lipolytic bacteria from the rumen of the sheep. J Gen Microbiol 25:227–240
    [Google Scholar]
  9. Holdeman L. V., Cato E. P., Moore W. E. C. 1977 Anaerobe Laboratory Manual, 4th. edn. Blacksburg, VA: Virginia Polytechnic Institute & State University Press;
    [Google Scholar]
  10. Holdeman L. V., Kelley R. W., Moore W. E. C. 1984; Family I. Bacteroidaceae Pribram 1933, 10AL*. In Bergeys Manual of Systematic Bacteriology vol. 1 pp. 602–603 Edited by Krieg N. R., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  11. Hungate R. E. 1966 The Rumen and its Microbes. London: Academic Press;
    [Google Scholar]
  12. Hungate R. E. 1969; A roll tube method for cultivation of strict anaerobes. Methods Microbiol 3B:117–132
    [Google Scholar]
  13. Kingsley V. V., Hoeniger J. F. M. 1973; Growth, structure and classification of Selenomonas. Bacteriol Rev 37:479–521
    [Google Scholar]
  14. Lee S. Y., Mabee M. S., Jangaard N. O. 1978; Pectinatus, a new genus of the family Bacteroidaceae. Int J Syst Bacteriol 28:582–594
    [Google Scholar]
  15. Maidak B. L., Olsen G. J., Larsen N., Overbeek R., McCaughey M. J., Woese C. R. 1996; The Ribosomal Database Project. Nucleic Acids Res 24:82–85
    [Google Scholar]
  16. Mandel M., Marmur J. 1968; Use of ultraviolet absorbance-temperature profile for determining the guanine plus cytosine content of DNA. Methods Enzymol 12B:195
    [Google Scholar]
  17. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3:208–218
    [Google Scholar]
  18. Miller T. L., Wolin M. J. 1974; A serum bottle modification of Hungate technique for cultivating obligate anaerobes. Appl Microbiol 27:985–987
    [Google Scholar]
  19. Moore E. R. B., Wittich R. M., Fortnagel P., Timmis K. N. 1993; 16S ribosomal RNA gene sequence characterization and phylogeneric analysis of dibenzo-p-dioxin degrading isolate within the new genus Sphingomonas. Lett Appl Microbiol 17:115–118
    [Google Scholar]
  20. Ricke S. C., Martin S. A., Nisbet D. J. 1996; Ecology, metabolism and genetics of ruminal Selenomonads. Crit Rev Microbiol 2227–65
    [Google Scholar]
  21. Sarnaik S., Godbole S. H., Kanekar P. 1989; Microbiological status of groundnut oil in Pune market. J Food Sci Technol 26:337–340
    [Google Scholar]
  22. Schauder R., Schink B. 1989; Anaerovibrio glycerini sp. nov., an anaerobic bacterium fermenting glycerol to propionate, cell matter, and hydrogen. Arch Microbiol 152:473–478
    [Google Scholar]
  23. Touzel J. P., Albagnac G. 1983; Isolation and characterization of Methanococcus mazei strain MC3 . FEMS Microbiol Lett 16:241–245
    [Google Scholar]
  24. Winteler H. V., Schneidinger B., Jaeger K. E., Haas D. 1996; Anaerobically controlled expression system derived from the arcDABC operon of Pseudomonas aeruginosa: application to lipase production. Appl Environ Microbiol 62:3391–3398
    [Google Scholar]
  25. Wolin E. A., Wolin M. J., Wolfe R. S. 1963; Formation of methane by bacterial extracts. J Biol Chem 238:2882–2886
    [Google Scholar]
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