Microvirgula aerodenitrificans gen. nov., sp. nov., a new Gram-negative bacterium exhibiting co-respiration of oxygen and nitrogen oxides up to oxygen-saturated conditions
A denitrifier micro-organism was isolated from an upflow denitrifying filter inoculated with an activated sludge. The cells were Gram-negative, catalase-and oxidase-positive curved rods and very motile. They were aerobic as well as anoxic heterotrophs that had an atypical respiratory type of metabolism in which oxygen and nitrogen oxides were used simultaneously as terminal electron acceptors. The G+C content was 65 mol%. Our isolate was phenotypically similar to Comamonas testosteroni, according to classical systematic classification systems. However, a phylogenetic analysis based on the 16S rRNA sequence showed that the aerobic denitrif could not be assigned to any currently recognized genus. For these reasons a new genus and species, Microvirgula aerodenitrificans gen. nov., sp. nov., is proposed, for which SGLY2Tis the type strain.
AmannR. I.,
LudwigW.,
SchleiferK.-H.1995; Phylo-genetic identification and in situ detection of individual microbial cells without cultivation. Microbiol Rev 59:143–169
AmannR. I.1995; In situ identification of micro-organisms by whole cell hybridization with rRNA-targeted nucleic acid probes. In Molecular Microbial Ecology Manual1–15AkkermansA. D.,
van ElsasJ. D.,
de BruijnF. J.
Dordrecht: Kluwer Academic Publishers;
BrosiusJ.,
DullT. L.,
SleeterD. D.,
NoIlerH. F.1981; Gene organization and primary structure of a ribosomal RNA operon from Escherichia coli. J Mol Biol 148:107–127
GodonJ. J.,
ZumsteinE.,
DabertP.,
HabouzitF.,
MolettaR.1997; Molecular microbial diversity in an anaerobic digestor as determined by small-subunit rDNA sequence analysis. Appl Environ Microbiol 63:2802–2813
GrimesD. J.,
WoeseC. R.,
MacDonellM. T.,
ColwellR. R.1997; Systematic study of the genus Vogesella gen. nov. and its type species, Vogesella indigofera comb. nov. Int J Syst Bacteriol 47:19–27
HoltJ. G.,
KriegN. R.,
SneathP. H. A.,
StaleyJ. T.,
WilliamsS. T.
(editors) 1994Bergey’s Manual of Determinative Bacteriology, 9. Baltimore: Williams & Wilkins;
PatureauD.,
BernetN.,
MolettaR.1995; Study of the denitrifying enzymatic system of Comamonas sp., strain SGLY2, under various aeration conditions with a particular view on nitrate and nitrite reductases. Curr Microbiol 32:25–32
PatureauD.,
BernetN.,
MolettaR.1995; Effect of oxygen on denitrification in continuous chemostat culture with Comamonas sp. strain SGLY2. J Ind Microbiol 16:124–128
PatureauD.,
BernetN.,
MolettaR.1995; Combined nitrification and denitrification in a single aerated reactor using the aerobic denitrifier Comamonas sp. strain SGLY2. Water Res 31:1363–1370
RaskinL.,
PoulsenL. K.,
NogueraD. R.,
RittmannB. E.,
StahlD.1994; Quantification of methanogenic groups in anaerobic biological reactors by oligonucleotide probe hybridization. Appl Environ Microbiol 60:1241–1248
RobertsonL. A.,
Van NielE. W. J.,
TorresmansR. A. M.,
KuenenJ. G.1988; Simultaneous nitrification and denitrification in aerobic chemostat cultures of Thiosphaera pantotropha. Appl Environ Microbiol 54:2812–2818
RobertsonL. A.,
CornelisseR.,
De VosP.,
HadioetomoR.,
KuenenJ. G.1989; Aerobic denitrification in various heterotrophic nitrifiers. Antonie Leeuwenhoek 56:289–299
RobertsonL. A.,
DalsgaardT.,
RevsbechN. P.,
KuenenJ. G.1995; Confirmation of ‘aerobic denitrification’ in batch cultures, using gas chromatography and 15N mass spectrometry. FEMS Microbiol Ecol 18:113–120
StahlD. A.,
AmannR. I.1991; Development and application of nucleic acid probes in bacterial systematics. In Sequencing and Hybridization Techniques in Bacterial Systematics205–248StackebrandtE.,
GoodfellowM.
Chichester: Wiley;
TamaokaJ.,
HaD. M.,
KomagataK.1987; Reclassification of Pseudomonas acidovorans den Dooren de Jong 1926 and Pseudomonas testosteroni Marcus and Talalay 1956 as Comamonas acidovorans comb. nov. and Coma- monas testosteroni comb. nov., with an emended description of the genus Comamonas. Int J Syst Bacteriol 37:52–59
Microvirgula aerodenitrificans gen. nov., sp. nov., a new Gram-negative bacterium exhibiting co-respiration of oxygen and nitrogen oxides up to oxygen-saturated conditions