1887

Abstract

The nitrogen-fixing rhizobial symbionts of growing in the nature reserve at the Sierra de Huautla, Mexico, were isolated and characterized. All 104 isolates together with the type strain for , HAMBI 540, had similar 16S rRNA genes as revealed by PCR-RFLP analysis. Similarity in the sequences of the 16S rRNA genes placed the isolates on a phylogenetic branch shared with Among 66 randomly selected isolates, three closely related electrophoretic alloenzyme types (ETs) were identified, which were distinct from 10 ETs distinguished among 23 strains of A new species , represented by the isolate S02, is proposed based upon low estimates of DNA relatedness between our chosen type strain and the type strains for the other species, the dissimilarity of the nucleotide sequence of the 16S rRNA genes, and their distinct ETs compared with The description of is significant because in the reconstruction of the phylogeny of there was a shift in the node of the branch of relative to that of The revised phylogenetic tree would tend to indicate common ancestry between and .

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1998-07-01
2024-06-23
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