1887

Abstract

Strain Tab4ca helical prokaryote that was isolated from the body of a Haematopota sp. fly collected in Champchevrier, Indre-et-Loire, Touraine, France, was found to be a member of the class . The cells of strain Tab4cwere small, motile helices that were devoid of a wall. The organism passed through filters with mean pore diameters as small as 0.20 mm. Strain Tab4cgrew rapidly in liquid SP-4 medium at both 30 and 37 °C. The organism fermented glucose but did not hdrolyse arginine or urea, and did not require serum for growth. In preliminary electrophoretic analyses, the cell protein patterns of strain Tab4cwere distinct from those of 14 other spiroplasmas found in mosquitoes, deer flies and horse flies from Europe and the Far-East. In reciprocal metabolism inhibition and deformation serological tests, employing antigens and antisera representative of spiroplasma groups l-XXXIII (including all sub-groups), plus ungrouped strains BARC 1901 and BARC 2649, no serological relationship with Tab4cwas found. The G+C content of the DNA of strain Tab4cwas about 25·1 mol% and its genome size was 1.305 kbp. It is proposed that spiroplasma strain Tab4cbe assigned to group XVII (presently vacant) and that strain (ATCC 700271) is the type strain of a new species, Spiroplasma turonicum.

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1998-04-01
2022-05-23
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