1887

Abstract

Three spherical thermoacidophilic archaea (strains TA-1, TA–13, TA–14) were obtained from acidic hot springs located in Ohwaku Valley, Hakone, Japan. All the isolates are facultatively anaerobic, and grew optimally at around 85 °C pH 2·0. Isolate TA-1was characterized further. The G+C content of DNA from TA-1T is 33 mol%. Although these properties resemble those of the genus Acidianus, the sequence of the 165 rRNA gene from strain TA-1was more similar to that of species of Stygiolobus than of Acidianus. DNA-DNA hybridization experiments also indicated that strain TA-1is clearly distinguished phylogenetically from the members of Acidianus, Sulfolobus and Metallosphaera. On the basis of the distinct physiological and molecular properties, we describe the new strains as members of the new genus Sulfurisphaera. The type species of the genus is Sulfurisphaera ohwakuensis, and the type strain of the species is TA-1(= IFO 151 SI1).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-48-2-451
1998-04-01
2023-02-08
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/48/2/ijsem-48-2-451.html?itemId=/content/journal/ijsem/10.1099/00207713-48-2-451&mimeType=html&fmt=ahah

References

  1. Balch W. E., Fox G. E., Magrum L. J., Woese C. R., Wolfe R. S. 1979; Methanogens: re-evaluation of a unique biological group. Microbiol Rev 43:260–296
    [Google Scholar]
  2. Brierley C. L., Brierley J. A. 1973; A chemoauto trophic and thermophilic microorganism isolated from an acid hot spring. Can J Microbiol 19:183–188
    [Google Scholar]
  3. Brock T. D., Brock K. M., Belly R. T., Weiss R. L. 1972; Sulfolobus’. a new genus of sulfur-oxidizing bacteria living at low pH and high temperature. Arch Mikrobiol 84:54–68
    [Google Scholar]
  4. Dempsey G. P., Bullivant S. 1976; A copper block method for freezing non-cryoprotected tissue to produce ice-crystal-free regions for electron microscopy. J Microsc 106:251–260
    [Google Scholar]
  5. De Rosa M., Gambacorta A. 1988; The lipids of archae-bacteria. Prog Lipid Res 27:153–157
    [Google Scholar]
  6. Felsenstein J. 1981; Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376
    [Google Scholar]
  7. Felsenstein J. 1993; phylip (Phylogeny Inference Package) version 3.5c. Distributed by the author. Department of Genetics, University of Washington; Seattle:
    [Google Scholar]
  8. Fuchs T., Huber H., Teiner K., Burggraf S., Stetter K. O. 1995; Metallosphaera prunae, sp. nov., a novel metal-mobilizing, thermoacidophilic Archaeum, isolated from a uranium mine in Germany. Syst Appl Microbiol 18:560–566
    [Google Scholar]
  9. Fuchs T., Huber H., Burggraf S., Stetter K. O. 1996; 16S rDNA-based phylogeny of the archaeal order Sulfolobales and reclassification of Desulfurolobus ambivalens as Acidianus ambivalens comb. nov. Syst Appl Microbiol 19:56–60
    [Google Scholar]
  10. Gambacorta A., Gliozzi A., De Rosa M. 1995; Archaeal lipids and their biotechnological applications. World J Microbiol Biotech 11:115–131
    [Google Scholar]
  11. Grogan D., Palm P., Zillig W. 1990; Isolate B12, which harbors a virus-like element, represents a new species of the archaebacterial genus Sulfolobus, Sulfolobus shibatae, sp. nov. Arch Microbiol 154:594–599
    [Google Scholar]
  12. Huber G, Stetter K. O. 1991; Sulfolobus metallicus, sp. nov., a novel strictly chemolithoautotrophic thermophilic archaeal species of metal-mobilizers. Syst Appl Microbiol 14:372–378
    [Google Scholar]
  13. Huber G., Spinnler C., Gambacorta A., Stetter K. O. 1989; Metallosphaera sedula gen. and sp. nov. represents a new genus of aerobic, metal-mobilizing, thermoacidophilic archaebacteria. Syst Appl Microbiol 12:38–47
    [Google Scholar]
  14. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120
    [Google Scholar]
  15. Kruczak-Filipov P., Shively R. G. 1992; Gram stain procedure. Clinical Microbiology Procedures Handbook 11.5.1–1.5.18 Edited by Isenberg H. D. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  16. Kurosawa N., Itoh Y. H. 1993; Nucleotide sequence of the 16S rRNA gene from thermoacidophilic archaea Sulfolobus acidocaldarius ATCC33909. Nucleic Acids Res 21:357
    [Google Scholar]
  17. Kurosawa N., Ohkura K., Horiuchi T., Itoh Y. H. 1995; Characterization of the 16S ribosomal RNA genes and phylogenetic relationships of sulfur-dependent thermoacidophilic archaebacteria. J Gen Appl Microbiol 41:75–81
    [Google Scholar]
  18. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  19. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: a Laboratory Manual, 2nd. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  20. Segerer A., Neuner A., Kristjansson J. K., Stetter K. O. 1986; Acidianus infernus gen. nov., sp. nov., and Acidianus brierleyi comb. nov.: facultatively aerobic, extremely acidophilic thermophilic sulfur-metabolizing archaebacteria. Int J Syst Bacteriol 36:559–564
    [Google Scholar]
  21. Segerer A., Trincone A., Gahrtz M., Stetter K. O. 1991; Stygiolobus azoricus gen. nov., sp. nov. represents a novel genus of anaerobic, extremely thermoacidophilic archaebacteria of the order Sulfolobales. Int J Syst Bacteriol 41:495–501
    [Google Scholar]
  22. Stetter K. O., König H., Stackebrandt E. 1983; Pyrodictium, a new genus of submarine disc-shaped sulfur reducing archaebacteria growing optimally at 105 °C. Syst Appl Microbiol 4:535–551
    [Google Scholar]
  23. Sugai A., Sakuma R., Fukuda I., Kurosawa N., Itoh Y. H., Kon K., Ando S., Itoh T. 1995; The structure of the core polyol of the ether lipids from Sulfolobus acidocaldarius. Lipids 30:339–344
    [Google Scholar]
  24. Takayanagi S., Kawasaki H., Sugimori K., Yamada T., Sugai A., Ito T., Yamasato K., Shioda M. 1996; Sulfolobus hakonensis sp. nov., a novel species of acidothermophilic archaeon. Int J Syst Bacteriol 46:377–382
    [Google Scholar]
  25. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reversed phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128
    [Google Scholar]
  26. Zillig W., Stetter K. O., Wunderl S., Schulz W., Priess H., Schulz I. 1980; The Sulfolobus-4 Caldariella ‘group: taxonomy on the basis of the structure of DNA-dependent RNA polymerases. Arch Microbiol 125:259–269
    [Google Scholar]
  27. Zillig W., Stetter K. O., Schäfer W., Janekovic D., Wunderl S., Holz J., Palm P. 1981; Thermoproteales: a novel type of extremely thermoacidophilic anaerobic archaebacteria isolated from Icelandic solfataras. Zentralbl Bakteriol Mikrobiol Hyg I Abt Orig C 2:205–227
    [Google Scholar]
  28. Zillig W., Stetter K. O., Prangishvilli D., Schäfer W., Wunderl S., Janekovic D., Holz I., Palm P. 1982; Desulfurococcaceae, the second family of the extremely thermophilic, anaerobic, sulfur-respiring Thermoproteales. Zentralbl Bakteriol Mikrobiol Hyg I Abt OrigC3304–317
    [Google Scholar]
  29. Zillig W., Gierl A., Schreiber G., Wunderl S., Janekovic D., Stetter K. O., Klenk H. P. 1983a; The archaebacterium Thermophilum pendens represents a novel genus of the thermophilic, anaerobic sulfur respiring Thermoproteales. Syst Appl Microbiol 4:79–87
    [Google Scholar]
  30. Zillig W., Holz I., Janekovic D., Schäfer W., Reiter W. D. 1983b; The archaebacterium Thermococcus celer represents a novel genus within the thermophilic branch of the archaebacteria. Syst Appl Microbiol 4:88–94
    [Google Scholar]
  31. Zillig W., Yeats S., Holz I., Böck A., Rettenberger M., Gropp F., Simon G. 1986; Desulfurolobus ambivalens, gen. nov., sp. nov., an autotrophic archaebacterium facultatively oxidizing or reducing sulfur. Syst Appl Microbiol 8:197–203
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-48-2-451
Loading
/content/journal/ijsem/10.1099/00207713-48-2-451
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error