1887

Abstract

An HPLC procedure which separates -and -amino acid isomers was applied to an analysis of peptidoglycan of 2,4-diaminobutyric acid (DAB)-containing actinomycetes. The cell wall peptidoglycans of species of the genera and contain DAB and have been differentiated principally by their menaquinone profile. These peptidoglycans are known to be identical in structure, all being of the B2γ type, possessing both -and -DAB. The type strains of all the subspecies of have -and -DAB in almost equal proportions in their cell wall peptidoglycan as previously reported. In contrast, the type strains of and all valid species of the genera and contain the -isomer of DAB almost exclusively. This characteristic is in good agreement with phylogenetic analyses based on 16S rDNA sequences and menaquinone profiles. On the basis of these data, the transfer of to the genus as comb. nov. is proposed. The isomer profile of DAB is shown to be a good taxonomic marker to differentiate these genera.

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1998-04-01
2024-04-16
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