Skip to content
1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 48, Issue 2

Taxonomic significance of 2,4-diaminobutyric acid isomers in the cell wall peptidoglycan of actinomycetes and reclassification of as toxicus comb. nov. Free

Abstract

An HPLC procedure which separates -and -amino acid isomers was applied to an analysis of peptidoglycan of 2,4-diaminobutyric acid (DAB)-containing actinomycetes. The cell wall peptidoglycans of species of the genera and contain DAB and have been differentiated principally by their menaquinone profile. These peptidoglycans are known to be identical in structure, all being of the B2γ type, possessing both -and -DAB. The type strains of all the subspecies of have -and -DAB in almost equal proportions in their cell wall peptidoglycan as previously reported. In contrast, the type strains of and all valid species of the genera and contain the -isomer of DAB almost exclusively. This characteristic is in good agreement with phylogenetic analyses based on 16S rDNA sequences and menaquinone profiles. On the basis of these data, the transfer of to the genus as comb. nov. is proposed. The isomer profile of DAB is shown to be a good taxonomic marker to differentiate these genera.

  • Published Online:
Keyword(s): 2,4-diaminobutyric acid isomers , actinomycetes , Clavibacter toxicus , peptidoglycan and Rathayibacter toxicus comb. nov.
© 1998, International Union of Microbiological Societies
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-48-2-403
1998-04-01
2025-04-29
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/48/2/ijsem-48-2-403.html?itemId=/content/journal/ijsem/10.1099/00207713-48-2-403&mimeType=html&fmt=ahah

References

  1. Bendinger B., Kroppenstedt R. M., Klatte S., Altendorf K. 1992; Chemotaxonomic differentiation of coryneform bacteria isolated from biofilters. Int J Syst Bacteriol 42:474–486
     [Google Scholar]
  2. Collins M. D. 1982; Lipid composition of Agromyces ramosus (Gledhill and Casida). FEMS Microbiol Lett 14187–189
     [Google Scholar]
  3. Collins M. D. 1983; Cell wall peptidoglycan and lipid composition of the phytopathogen Corynebacterium rathayi (Smith). Syst Appl Microbiol 4:193–198
     [Google Scholar]
  4. Collins M. D., Jones D. 1980; Lipids in the classification and identification of coryneform bacteria containing peptidoglycans based on 2,4-diaminobutyric acid. J Appl Bacteriol 48:459–470
     [Google Scholar]
  5. Davis M. J., Gillaspie A. G. Jr, Vidaver A. K., Harris R. W. 1984; Clavibacter: a new genus containing some phyto- pathogenic coryneform bacteria, including Clavibacter xyli subsp. xyli sp. nov., subsp. nov. and Clavibacter xyli subsp. cynodontis subsp. nov., pathogens that cause ratoon stunting disease of sugarcane and Bermudagrass stunting disease. Int J Syst Bacteriol 34107–117
     [Google Scholar]
  6. Dôpfer H., Stackebrandt E., Fiedler F. 1982; Nucleic acid hybridization studies on Microbacterium, Curtobacterium, Agromyces and related taxa. J Gen Microbiol 128:1697–1708
     [Google Scholar]
  7. Einarsson S., Josefsson B. 1987; Separation of amino acid enantiomers and chiral amines using precolumn derivatization with (+)-l-(9-fluorenyl)ethyl chloroformate and reversed-phase liquid chromatography. Anal Chem 59:1191–1195
     [Google Scholar]
  8. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229
     [Google Scholar]
  9. Fiedler F., Kandler O. 1973; Die Aminosauresequenz von 2,4- diaminobuttersâure enthaltenden mureinen bei verschiedenen coryneformen bakterien und Agromyces ramosus. Arch Mikro- biolS951–66
     [Google Scholar]
  10. Fiedler F., Schleifer K. H., Cziharz B., Interschick E., Kandler O. 1970; Murein types in Arthrobacter, Brevibacteria, Coryne-bacteria and Microbacteria. Publ Fac Sci Univ J E Purkyne Brno 47:111–122
     [Google Scholar]
  11. Gledhill W. E., Casida L. E. Jr 1969; Predominant catalasenegative soil bacteria. Appl Microbiol 17:208–213
     [Google Scholar]
  12. Groth I., Schumann P., Weiss N., Martin K., Rainey F. A. 1996; Agrococcus jenensis gen. nov., sp. nov., a new genus of actinomycètes with diaminobutyric acid in the cell wall. Int J Syst Bacteriol 46:234–239
     [Google Scholar]
  13. Hayashi T., Sasagawa T. 1993; A method for identifying the carboxy terminal amino acid of protein. Anal Biochem 209:163–168
     [Google Scholar]
  14. Hensel R. 1984; Three new murein types in coryneform bacteria isolated from activated sludge. Syst Appl Microbiol 5:11–19
     [Google Scholar]
  15. Inoue K., Komagata K. 1976; Taxonomic study on obligately psychrophilic bacteria isolated from Antarctica. J Gen Microbiol 22:165–176
     [Google Scholar]
  16. Komagata K., Suzuki K. 1987; Lipid and cell wall analysis in bacterial systematics. Methods Microbiol 19:161–208
     [Google Scholar]
  17. Leifson E. 1962; The bacterial flora of distilled and stored water. III. New species of the genera Corynebacterium, Flavobacterium, Spirillum and Pseudomonas. Int Bull Bacteriol Nomenci Taxon 12:161–170
     [Google Scholar]
  18. Mamoli L. 1939; Über biochemische Dehydrierungen in der Cortingruppe. Ber Dtsch Chem Ges 72:1863–1865
     [Google Scholar]
  19. Puchala R., Pior H., Kulasek G. W., Shelford J. A. 1992; Determination of diaminopimelic acid in biological materials using high-performance liquid chromatography. J Chromatogr 623:63–67
     [Google Scholar]
  20. Rainey F., Weiss N., Prauser H., Stackebrandt E. 1994; Further evidence for the phylogenetic coherence of actinomycètes with group B-peptidoglycan and evidence for the phylogenetic intermixing of the genera Microbacterium and Aureobacterium as determined by 16S rDNA analysis. FEMS Microbiol Lett 118:135–140
     [Google Scholar]
  21. Riley I. T., Ophel K. M. 1992; Clavibacter toxicus sp. nov., the bacterium responsible for annual ryegrass toxicity in Australia. Int J Syst Bacteriol 42:64–68
     [Google Scholar]
  22. Saito H., Miura K. 1963; Preparation of transforming deoxyribonucleic acid by phenol treatment. Biochim Biophys Acta 72:619–629
     [Google Scholar]
  23. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
     [Google Scholar]
  24. Schleifer K. H., Kandler O. 1972; Peptidoglycan type of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36:407–477
     [Google Scholar]
  25. Suzuki K., Sasaki J., Uramoto M., Nakase T., Komagata K. 1996; Agromyces mediolanus sp. nov., nom. rev., comb, nov., a species for ‘Corynebacterium mediolanum’ Mamoli 1939 and for some aniline-assimilating bacteria which contain 2,4- diaminobutyric acid in the cell wall peptidoglycan. Int J Syst Bacteriol 46:88–93
     [Google Scholar]
  26. Suzuki K., Sasaki J., Uramoto M., Nakase T., Komagata K. 1997; Cryobacterium psychrophilum gen. nov., sp. nov., nom. rev., comb, nov., an obligately psychrophilic actinomycete to accommodate “Curtobacterium psychrophilum’ Inoue and Komagata 1976. Int J Syst Bacteriol AT474–478
     [Google Scholar]
  27. Takahashi Y., Iwai Y., Tomoda H., Nimura N., Kinoshita T., Omura S. 1989; Optical resolution of 2,6-diaminopimelic acid stereoisomers by high performance liquid chromatography for the chemotaxonomy of actinomycete strains. J Gen Appl Microbiol 35:27–32
     [Google Scholar]
  28. Takeuchi M., Weiss N., Schumann P., Yokota A. 1996; Leucobacter komagatae gen. nov., sp. nov., a new aerobic Gram-positive, nonsporulating rod with 2,4-diaminobutyric acid in the cell wall. Int J Syst Bacteriol 46:967–971
     [Google Scholar]
  29. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128
     [Google Scholar]
  30. Thompson J. D., Higgins D. G., Gibson T. J. 1994; clustal w: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680
     [Google Scholar]
  31. Tisdall P. A., Anhalt J. P. 1979; Rapid differentiation of Streptomyces from Nocardia by liquid chromatography. J Clin Microbiol 10:503–505
     [Google Scholar]
  32. Yamada K., Komagata K. 1972a; Taxonomic studies on coryneform bacteria IV. Morphological, cultural, biochemical and physiological characteristics. J Gen Appl Microbiol 18:399–416
     [Google Scholar]
  33. Yamada K., Komagata K. 1972b; Taxonomic studies on coryneform bacteria V. Classification of Coryneform bacteria. J Gen Appl Microbiol 18:417–431
     [Google Scholar]
  34. Zgurskaya H. I., Evtushenko L. I., Akimov V. N., Voyevoda H. V., Dobrovolskaya T. G., Lysak L. V., Kalakoutskii L. V. 1992; Emended description of the genus Agromyces and description of Agromyces cerinus subsp. cerinus sp. nov., subsp. nov., Agromyces cerinus subsp. nitratus sp. nov., subsp. nov., Agromyces fucosus subsp. fucosus sp. nov., subsp. nov., Agromyces fucosus subsp. hippuratus sp. nov., subsp. nov. Int J Syst Bacteriol 42:635–641
     [Google Scholar]
  35. Zgurskaya H. I., Evtushenko L. I., Akimov V. N., Kalakoutskii L. V. 1993; Rathayibacter gen. nov., including the species Rathayibacter rathayi comb, nov., Rathayibacter tritici comb, nov., Rathayibacter iranicus comb, nov., and six strains from annual grasses. Int J Syst Bacteriol 43:143–149
     [Google Scholar]
/content/journal/ijsem/10.1099/00207713-48-2-403
Loading
Taxonomic significance of 2,4-diaminobutyric acid isomers in the cell wall peptidoglycan of actinomycetes and reclassification of Clavibacter toxicus as Rathayibacter toxicus comb. nov.
Int J Syst Evol Microbiol 48, 403 (1998); https://doi.org/10.1099/00207713-48-2-403
/content/journal/ijsem/10.1099/00207713-48-2-403
/content/journal/ijsem/10.1099/00207713-48-2-403
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error