1887

Abstract

Four moderately thermophilic, carboxydotrophic streptomycetes were the subject of a comparative taxonomic investigation designed to establish their taxonomic relationships. Almost complete sequences of the 16S rRNA genes of the test strains were determined following the isolation and direct sequencing of the amplified genes. The resultant nucleotide sequences were aligned with the sequences of previously studied streptomycetes, and phylogenetic trees generated by using the neighbour-joining, Fitch-Margoliash, maximum-likelihood and maximum-parsimony methods. It was evident from the phylogenetic analyses that strains AT50, AT51 and AT52 were most closely related to DSM 40573 and strain AT37 to DSM 40716 and NRRL 3985. Random DNA amplification profiles clearly distinguished strains AT50, AT51 and AT52 from and from strain AT37. The molecular systematic evidence, together with phenotypic data derived from this and previous studies, indicate that the test strains merit species status within the genus . The name sp. nov. is proposed for strains AT50, AT51 and AT52 (type strain) and sp. nov. for strain AT37.

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1998-01-01
2024-02-23
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