1887

Abstract

Strawberry plants exhibiting symptoms of stunting and abnormally small leaves were observed in production fields in central Florida, USA. Since the symptoms were suggestive of phytoplasma infection, plants were assayed for presence of phytoplasma by PCR amplification of 16S rDNA and ribosomal protein (rp) gene sequences. Amplification of phytoplasma-specific DNA sequences by PCR indicated infection of the diseased strawberry plants by phytoplasmas. RFLP analyses of amplified 16S rDNA revealed that the plants were infected by two mutually distinct phytoplasmas that differed from strawberry green petal phytoplasma (group 16Srl-C). Both phytoplasmas were members of 16S rRNA gene group I (16Srl). Based on RFLP analysis of amplified 16S rDNA and rp gene sequences, one was classified in group 16Srl subgroup I and new rp subgroup 16Srl-l(rp); its 16S rRNA-rp subgroup was designated 16Srl-K(rr-rp). The second phytoplasma represented a previously undescribed subgroup, designated K, in 16S rRNA group I but belonged to rp subgroup 16Srl-J(rp); this phytoplasma's 16S rRNA-rp subgroup was designated 16Srl- J(rr-rp). Results of RFLP analyses agreed with putative restriction site maps based on nucleotide sequences determined for the amplified 16S rDNAs and rp gene operon DNAs. Further evidence indicated that the 16Srl-K(rr-rp) strawberry phytoplasma, Mexican periwinkle virescence phytoplasma and stolbur phytoplasma shared sequence homologies that enabled amplification of DNA from all three by PCR using primers previously designed as stolbur- specific.

Keyword(s): multiplier disease and mycoplasma
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-48-1-269
1998-01-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/48/1/ijs-48-1-269.html?itemId=/content/journal/ijsem/10.1099/00207713-48-1-269&mimeType=html&fmt=ahah

References

  1. Barry T., Colleran G., Glennon M., Dunican L. K., Gannon F. 1991; The 16S/23S ribosomal spacer region as a target for DNA probes to identify eubacteria. PCR Methods Appl 151–56
    [Google Scholar]
  2. Davis R. E., Dally E. L., Gundersen D. E., Lee l.-M., Habili N. 1997; ‘Candidatus Phytoplasma australiense’, a new phytoplasma taxon associated with Australian grapevine yellows. Int J Syst Bacteriol 47262–269
    [Google Scholar]
  3. Gundersen D. E., Lee l.-M. 1996; Ultrasensitive detection of phytoplasmas by nested-PCR assays using two universal primer pairs. Phytopathol Mediten 35144–151
    [Google Scholar]
  4. Gundersen D. E., Lee l.-M., Rehner S. A., Davis R. E., Kingsbury D. T. 1994; Phylogeny of mycoplasmalike organisms (phytoplasmas): a basis for their classification. J Bacteriol 1765244–5254
    [Google Scholar]
  5. Gundersen D. E., Lee l.-M., Schaff D. A., Harrison N. A., Chang C. J., Davis R. E., Kingsbury D. T. 1996; Genomic diversity and differentiation among phytoplasma strains in 16S rRNA group I (aster yellows and related strains) and group III (peach X-disease and related strains). Int J Syst Bacteriol 4664–75
    [Google Scholar]
  6. Harrison N. A., Legard D. E., DiBonito R., Richardson P. A. 1997; Detection and differentiation of phytoplasmas associated with diseases of strawberry in Florida. Plant Dis 81230
    [Google Scholar]
  7. Jomantiene R., Davis R. E., Maas J., Dally E. L. 1996; Phytoplasmas associated with disease of strawberry in Florida. Phytopathology 86S123
    [Google Scholar]
  8. Kirkpatrick B. C., Smart C., Gardner S., Gao J.-L., Ahrens U., Mäurer R., Schneider B., Lorenz K.-H., Seemüller, E„ Harrison N., Namba S., Daire X. 1994; Phylogenetic relationships of plant pathogenic MLOs established by 16/23S rDNA spacer sequences. IOM Lett 3228–229
    [Google Scholar]
  9. Lee I.-M., Hammond R. W., Davis R. E., Gundersen D. E. 1993; Universal amplification and analysis of pathogen 16S rDNA for classification and identification of mycoplasmalike organisms. Phytopathology 83834–842
    [Google Scholar]
  10. Lim P.-O., Sears B. B. 1992; Evolutionary relationships of a plant pathogenic mycoplasmalike organism and Achole- plasma laidlawii deduced from two ribosomal protein gene sequences. J Bacteriol 1742606–2611
    [Google Scholar]
  11. Maas J. L. (editor) 1984 Compendium of Strawberry Diseases St Paul, MN: APS Press;
    [Google Scholar]
  12. Maixner M., Ahrens U., Seemüller E. 1995; Detection of the German grapevine yellows (Vergilbungskrankheit) MLO in grapevine, alternative hosts, and a vector by a specific PCR procedure. Eur J Plant Pathol 101241–250
    [Google Scholar]
  13. Padovan A. C., Gibb K. S., Bertaccini A., Vibio M., Bon- figlioli R. E., Magarey P. A., Sears B. B. 1995; Molecular detection of the Australian grapevine yellows phytoplasma and comparison with grapevine yellows phytoplasmas from Italy. Aust J Grape Wine Res 125–31
    [Google Scholar]
  14. Schaff D. A., Lee l.-M., Davis R. E. 1992; Sensitive detection and identification of mycoplasmalike organisms by polymerase chain reactions. Biochem Biophys Res Commun 1861503–1509
    [Google Scholar]
  15. Seemüller E., Schneider B., Mäurer R., Ahrens U., Daire X., Kison H., Lorenz K.-H., Firrao G., Avinent L., Sears B. B., Stackebrandt E. 1994; Phylogenetic classification of phyto- pathogenic mollicutes by sequence analysis of 16S ribosomal DNA. Int J Syst Bacteriol 44440–446
    [Google Scholar]
  16. Smart C. D., Schneider B., Blomquist C. L., Guerra L. J., Harrison N. A., Ahrens U., Lorenz K.-H., Seemüller E., Kirkpatrick B. C. 1996; Phytoplasma-specific PCR primers based on sequences of the 16S-23SrRNA spacer region. Appi Environ Microbiol 622988–2993
    [Google Scholar]
  17. Toth K. F., Harrison N. A., Sears B. B. 1994; Phylogenetic relationships among members of the class Mollicutes deduced from rps3 gene sequences. Int J Syst Bacteriol 44119–124
    [Google Scholar]
  18. Vibio M., Bertaccini A., Lee l.-M., Davis R. E., Clark M. F. 1996; Differentiation and classification of aster yellows and related European phytoplasmas. Phytopathol Mediterr 3533–42
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-48-1-269
Loading
/content/journal/ijsem/10.1099/00207713-48-1-269
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error