1887

Abstract

Marginal chlorosis is a new disease of strawberry which was first seen in France in 1988. A phloem-restricted bacterium-like organism was found associated with the disease. Even though the organism could not be cultured, and resembles in this way most other phloem-restricted pathogens, characterization was achieved from the sequence of its PCR-generated 16S rDNA, and comparison with other organisms. From these studies, the strawberry agent was found to be a new bacterium within group 3 of the gamma subclass of a group of Gram-negative bacteria including, in particular, insect symbionts or parasites as well as enterobacteria. Its closest relative, is the causal agent of the son- killer trait in wasps. The two bacteria share 92% 16S rDNA sequence identity. We propose a taxon for the marginal-chlorosis-associated bacterium, Phlomobacter fragariae'.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-48-1-257
1998-01-01
2022-10-03
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/48/1/ijs-48-1-257.html?itemId=/content/journal/ijsem/10.1099/00207713-48-1-257&mimeType=html&fmt=ahah

References

  1. Bové J. M., Gamier M. 1997; Walled and wall-less eubacteria from plants: sieve-tube-restricted plant pathogens and plant-surface contaminants.. In Pathogen and Microbial Contamination Management in Micropropagation pp 45–60 Edited by Cassells A. C. Dordrecht: Kluwer Academic Publishers;
    [Google Scholar]
  2. Bové J. M., Whitcomb R. F., McCoy R. E. 1983; Culture techniques for spiroplasmas from plants. Methods Myco- plasmol 2225–234
    [Google Scholar]
  3. Bruton B. D., Pair S. D., Wann E. V. 1995; Yellow vine disease of watermelon and Cantaloupe in Texas and Oklahoma.. Report of the US Department of Agriculture. Lane, OK: South Central Research Laboratory;
    [Google Scholar]
  4. Fos A., Danet J. L., Zreik L., Gamier M., Bové J. M. 1992; Use of a monoclonal antibody to detect the stolbur mycoplasmalike organism in plants and insects and to identify a vector in France. Plant Dis 761092–1096
    [Google Scholar]
  5. Gao S., Garnier M., Bové J. M. 1993; Production of monoclonal antibodies recognizing most strains of the greening BLO by in vitro immunization with an antigenic protein purified from the BLO.. In Proceedings of the 12th Conference of the International Organization of Citrus Virologists pp 244–249 Edited by Moreno P., da Graga J. V., Timmer L. W. Riverside, CA: c/o Department of Plant Pathology;
    [Google Scholar]
  6. Gamier M., Bové J. M. 1993; Citrus greening disease and the greening bacterium.. In Proceedings of the 12th Conference of the International Organization of Citrus Virologists pp 212–219 Edited by Moreno P., da Graga J. V., Timmer L. W. Riverside, CA: c/o Department of Plant Pathology;
    [Google Scholar]
  7. Gawel N. J., Jarret R. L. 1991; A modified CTAB DNA extraction procedure for Musa and ipomea. Plant Mol Biol Rep 9262–266
    [Google Scholar]
  8. Gherna R. L., Werren J. H., Weisburg W., Cote R., Woese C. R., Mandelco L., Brenner D. J. 1991; Arsenophonus nasoniae gen. nov., sp. nov., the causative agent of the son- killer trait in the parasitic wasp Nasonia vitripennis.. Int J Syst Bacteriol 41563–565
    [Google Scholar]
  9. Jagoueix S., Bové J. M., Garnier M. 1994; The phloem- limited bacterium of greening disease of citrus is a member of the a subdivision of the proteobacteria. Int J Syst Bacteriol 44379–386
    [Google Scholar]
  10. Jagoueix S., Bové J. M., Garnier M. 1996; PCR detection of the two liberobacter species associated with greening disease of citrus. Mol Cell Probes 1043–50
    [Google Scholar]
  11. Kirkpatrick B.C., Smart C. D., Blomquist C., Gurra L., Harrisson N., Ahrens U., Lorentz K. H., Schneider B., Seemüller E. 1994; Identification of MLO strains specific PCR primers obtained from 16/23S rRNA spacer sequences. 10M Lett 3261–262
    [Google Scholar]
  12. McCoy R. E., Caudwell A., Chang C. J. 16 other authors 1989; Plant diseases associated with mycoplasma-like organisms.. In The Mycoplasmas vol 5 Spiroplasmas, Acholeplasmas, and Mycoplasmas of Plants and Arthropods pp 545–640 Edited by Whitcomb R. F., Tully J. G. New York: Academic Press;
    [Google Scholar]
  13. Murray R. G. E., Schleifer K. H. 1994; Taxonomie notes: a proposal for recording the properties of putative taxa of procaryotes. Int J Syst Bacteriol 44174–176
    [Google Scholar]
  14. Nourrisseau J. G., Lansac M., Garnier M. 1993; Marginal chlorosis, a new disease of strawberries associated with a bacteriumlike organism. Plant Dis 771055–1059
    [Google Scholar]
  15. Olsen G. J., Larsen N., Woese C. R. 1991; The ribosomal RNA Database Project. Nucleic Acids Res 192017–2021
    [Google Scholar]
  16. Purcell A. H., Steiner T., Mégraud F., Bové J. M. 1986; In vitro isolation of a transovarially transmitted bacterium from the leafhopper Euscelidius variegatus (Hemiptera: Cicadellidae). J Invertebr Pathol 4866–73
    [Google Scholar]
  17. Villechanoux S., Garnier M., Renaudin J., Bové J. M. 1992; Detection of several strains of the bacterium-like organism of citrus greening disease by DNA probes. Curr Microbiol 2489–95
    [Google Scholar]
  18. Weisburg W. G., Barns S. M., Pelletier D. A., Lane D. J. 1991; 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 173697–703
    [Google Scholar]
  19. Werren J. H., Skinner S. W., Huger A. 1986; Male-killing bacteria in a parasitic wasp. Science 231990–992
    [Google Scholar]
  20. Woese C. R., Weisburg W. G., Hahn C. M., Paster B. J., Zahlen L. B., Lewis B. J., Macke T. J., Ludwig W., Stackebrandt E. 1985; The phylogeny of purple bacteria: the gamma subdivision. Syst Appl Microbiol 625–33
    [Google Scholar]
  21. Zreik L., Carle P., Bové J. M., Garnier M. 1995; Characterization of the mycoplasmalike organism associated with witches’-broom disease of lime and proposition of a Candidatus taxon for the organism, ‘ Candidatus Phytoplasma aurantifolia’. Int J Syst Bacteriol 45449–453
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-48-1-257
Loading
/content/journal/ijsem/10.1099/00207713-48-1-257
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error