Emended description of Campylobacter sputorum and revision of its infrasubspecific (biovar) divisions, including C. sputorum biovar paraureolyticus, a urease-producing variant from cattle and humans
A polyphasic taxonomic study of 15 bovine and human strains assigned to the catalase-negative, urease-positive campylobacter (CNUPC) group identified these bacteria as a novel, ureolytic biovar of Campylobacter sputorum for which we propose the name C. sputorum bv. paraureolyticus: suitable reference strains are LMG 11764 (human isolate) and LMG 17590 (=CCUG 37579, bovine isolate). The present study confirmed previous findings showing that the salient biochemical tests used to differentiate C. sputorum bv. sputorum from C. sputorum bv. bubulus are not reproducible; and that the absolute validity of source-specific biovars of the species is questionable. A correlation between the results of numerical analysis of protein profiles and the reaction of strains in certain enzyme tests was, however, noted. Therefore, it is proposed that the infrasubspecific (biovar) divisions of C. sputorum should be revised to include bv. sputorum for catalase-negative strains; bv. fecalis for catalase-positive strains; and bv. paraureolyticus for urease-positive strains. Strains classified previously as bv. bubulus should be reclassified as bv. sputorum. The species description of C. sputorum is revised accordingly.
AtabayH. I.,
CorryJ. E. L.,
OnS. L. W.1997; Isolation and characterization of a novel catalase-negative, urease-positive Campylobacter from cattle faeces. Lett Appl Microbiol 24:59–64
AtabayH. I.,
CorryJ. E. L.,
PostD. E.1996; Comparison of the productivity of a variety of selective media for Campylobacter and Arcobacter species. In Campylobacter VIII. Proceedings of the 8th International Workshop on Campylobacter, Helicobacter, and Related Organisms pp. 19–23 Edited by
NewellD. G.,
KetleyJ.,
FeldmanR. A.
New York: Plenum;
BastynsK.,
ChapelleS.,
VandammeP.,
GoossensH.,
De WachterR.1994; Species-specific detection of campylobacters important in veterinary medicine by PCR amplification of 23S rDNA areas. Syst Appl Microbiol 17:563–568
BorczykA.,
LiorH.,
McKeownA.,
SvendsenH.1988; Isolations of Campylobacter sputorum associated with human infections. In Campylobacter IV. Proceedings of the Fourth International Workshop on Campylobacter Infections, Goteborg, Sweden pp. 166–167 Edited by
KaijserB.,
FalsenE.
Sweden: University of Goteborg;
CharlierG.,
DekeyserP.,
FlorentA.,
StrobbeR.,
De LeyJ.1974; DNA base composition and biochemical characters of Campylobacter strains. Antonie Leeuwenhoek 40:145–151
CostasM.,
OwenR. J.,
JackmanP. J. H.1987; Classification of Campylobacter sputorum and allied campylobacters based on numerical analysis of electrophoretic protein patterns. Syst Appl Microbiol 9:125–131
GoodwinC. S.,
ArmstrongJ. A.,
ChilversT.,
PetersM.,
CollinsM.,
D„ SlyL.,
McConnellW.,
HarperW. E. S.1989; Transfer of Campylobacter pylori and Campylobacter mustelae to Helicobacter gen. nov. as Helicobacter pylori comb. nov. and Helicobacter mustelae comb. nov. respectively. Int J Syst Bacteriol 39:397–405
HarveyS. M.,
GreenwoodJ. R.1983; Relationships among catalase-positive campylobacters determined by deoxyribonucleic acid-deoxyribonucleic acid hybridization. Int J Syst Bacteriol 33:275–284
HolmesB.,
OnS. L. W.,
GannerM.,
CostasM.1992; Some new applications of probabilistic identification. In Proceedings of the 1992 Conference on Taxonomy and Automated Identification of Bacteria, Prague, Czechoslovakia pp. 6–9 Edited by
SchindlerJ.
Prague: Czechoslovak Society for Microbiology;
LawsonG. H. K.,
LeaverJ. L.,
PettigrewG. W.,
RowlandA. C.1981; Some features of Campylobacter sputorum subsp. mucosalis subsp. nov., nom. rev. and their taxonomic significance. Int J Syst Bacteriol 31:385–391
LindblomG.-B.,
SjdgrenE.,
Hansson-WesterbergJ.,
KaijserB.1995; Campylobacter upsaliensis, C. sputorum sputorum and C. concisus as common causes of diarrhoea in Swedish children. Scand J Infect Dis 27:187–188
OnS. L. W.,
BlochB.,
HolmesB.,
HosteB.,
VandammeP.1995; Campylobacter hyointestinalis subsp. lawsonii subsp. nov., isolated from the porcine stomach, and an emended description of Campylobacter hyointestinalis. Int J Syst Bacteriol 45:767–774
OnS. L. W.,
CostasM.,
HolmesB.1994; Classification and identification of Campylobacter sputorum using numerical analyses of phenotypic tests and of whole-cell protein profiles. Syst Appl Microbiol 17:543–553
OnS. L. W.,
HolmesB.1995; Classification and identification of campylobacters, helicobacters and allied taxa by numerical analysis of phenotypic tests. Syst Appl Microbiol 18:374–390
OnS.,
LW.,
HolmesB.,
SackinM.1996; A probability matrix for the identification of campylobacters, helicobacters, and allied taxa. J Appl Bacteriol 81:425–432
OnS. L. W.,
VandammeP.1997; Identification and epidemiological typing of Campylobacter hyointestinalis subspecies by phenotypic and genotypic methods and description of novel subgroups. Syst Appl Microbiol 20:238–247
PotB.,
VandammeP.,
KerstersK.1994; Analysis of electrophoretic whole-organism protein fingerprints. In Modern Microbial Methods (Chemical Methods in Prokaryotic Systematics series), pp. 493–521 Edited by
GoodfellowM.,
O’DonnellA. G.
Chichester: Wiley;
RoopR. M.,
II, SmibertR. M.,
JohnsonJ. L.,
KriegN. R.1985; DNA homology studies of the catalase-negative campylobacters and ‘ Campylobacter fecalis ’, an emended description of Campylobacter sputorum and proposal of the neotype strain of Campylobacter sputorum. Can J Microbiol 31:823–831
Van CampG.,
De PeerY. V.,
NicolaiS.,
NeefsJ.-M.,
VandammeP.,
De WachterR.1993; Structure of 16S and 23S ribosomal RNA genes in Campylobacter species: phylogenetic analysis of the genus Campylobacter and presence of internal transcribed spacers. Syst Appl Microbiol 16:361–368
VandammeP.,
DanashvarM. I.,
DewhirstF. E.,
PasterB. J.,
KerstersK.,
GoossensH.,
MossC. W.1995; Chemo- taxonomic analyses of Bacteroides gracilis and Bacteroides ureolyticus and reclassification of B. gracilis as Campylobacter gracilis comb. nov. Int J Syst Bacteriol 45:145–152
VandammeP.,
FalsenE.,
RossauR.,
HosteB.,
SegersP.,
TytgatR.,
De LeyJ.1991; Revision of Campylobacter, Helicobacter and Wolinella taxonomy: emendation of generic descriptions and proposal of Arcobacter gen. nov. Int J Syst Bacteriol 41:88–103
VandammeP.,
VancanneytM.,
PotB.,
MelsL.,
HosteB.,
DewettinckD.,
VlaesL.,
Van Den BorreC.,
HigginsR.,
KerstersK.,
ButzlerJ.-P.,
GoossensH.1992; Polyphasic taxonomic study of the emended genus Arcobacter with Arcobacter butzleri comb. nov. and Arcobacter skirrowii sp. nov., an aero tolerant bacterium isolated from veterinary specimens. Int J Syst Bacteriol 42:344–356
VandammeP.,
Van DoomL.-J.,
Al RashidS. T.,
QuintW. G. V.,
ChanV.,
L, Van der PladsJ.,
OnS. L. W.1997; Campylobacter hyoilei Alderton et al. 1995 and Campylobacter coli Veron and Chatelain 1973 are subjective synonyms. Int J Syst Bacteriol 47:1055–1060
VéronM.,
ChatelainR.1973; Taxonomic study of the genus Campylobacter Sebald and Veron and designation of the neotype strain for the type species, Campylobacter fetus (Smith and Taylor) Sebald and Veron. Int J Syst Bacteriol 23:122–134
WayneL. G.,
BrennerD. J.,
ColwellR. R.
9 other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464
Emended description of Campylobacter sputorum and revision of its infrasubspecific (biovar) divisions, including C. sputorum biovar paraureolyticus, a urease-producing variant from cattle and humans