Whole-cell protein electrophoretic analysis of viridans streptococci: evidence for heterogeneity among biovars Free

Abstract

One hundred reference strains representing all species belonging to the different phylogenetic lineages of the viridans streptococci were examined by means of one-dimensional whole-organism protein electrophoresis. For most of the species examined, multiple strains characterized by DNA-DNA hybridization were included and, wherever described, representatives of different biochemical variants were analysed. Most species were clearly differentiated. The data support the viewpoint that members of the group constitute a single species and indicate that biovar 2 is a heterogeneous taxon comprising strains from several streptococcal species.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-48-1-117
1998-01-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/48/1/ijs-48-1-117.html?itemId=/content/journal/ijsem/10.1099/00207713-48-1-117&mimeType=html&fmt=ahah

References

  1. Beighton D., Hayday H., Russell R. R. B., Whiley R. A. 1984; Streptococcus macacae sp. nov., from dental plaque of monkeys Macacae fascicular is. Int J Syst Bacteriol 34:332–335
    [Google Scholar]
  2. Bentley R. W., Leigh J. A., Collins M. D. 1991; Intrageneric structure of Streptococcus based on comparative analysis of small-subunit rRNA sequences. Int J Syst Bacteriol 41:487–494
    [Google Scholar]
  3. Carlsson J. 1968; A numerical taxonomic study of human oral streptococci. Odontol Revy 19:137–160
    [Google Scholar]
  4. Coykendall A. 1977; Proposal to elevate the subspecies of Streptococcus mutans to species status, based on their molecular composition. Int J Syst Bacteriol 27:26–30
    [Google Scholar]
  5. Coykendall A. 1983; Streptococcus sobrinus nom. rev. and Streptococcus ferus nom. rev.: habitat of these and other mutans streptococci. Int J Syst Bacteriol 33:883–885
    [Google Scholar]
  6. Coykendall A. L., Wesbecher P. M., Gustafson K. B. 1987; Streptococcus millerV, Streptococcus constellatus and Streptococcus intermedius are later synonyms of Streptococcus anginosus. Int J Syst Bacteriol 37:222–228
    [Google Scholar]
  7. Handley P., Coykendall A., Beighton D., Hardie J. M., Whiley R. A. 1991; Streptococcus crista sp. nov., a viridans streptococcus with tufted fibrils, isolated from the human oral cavity and throat. Int J Syst Bacteriol 41:543–547
    [Google Scholar]
  8. Jacobs J. A., Schot C. S., Bunschoten A. E., Schouls L. M. 1996; Rapid species identification of ‘Streptococcus miller V strains by line blot hybridization: identification of a distinct 16S rRNA population closely related to Streptococcus constellatus. J Clin Microbiol 34:1717–1721
    [Google Scholar]
  9. Kawamura Y., Hou X.-G., Sultana F., Miura H., Ezaki T. 1995; Determination of 16S rRNA sequences of Streptococcus mitis and Streptococcus gordonii and phylogenetic relationships among members of the genus Streptococcus. Int J Syst Bacteriol 45:406–408
    [Google Scholar]
  10. Kilian M., Mikkelsen L., Henrichsen J. 1989; Taxonomic study of viridans streptococci: description of Streptococcus gordonii sp. nov. and emended descriptions of Streptococcus sanguis (White and Niven 1946), Streptococcus oralis (Bridge and Sneath 1982), and Streptococcus mitis (Andrewes and Horder 1906). Int J Syst Bacteriol 39:471–484
    [Google Scholar]
  11. Magee J. T., Hindmarch J. M., Douglas C. W. I. 1997; A numerical taxonomic study of Streptococcus sanguis, Streptococcus mitis and similar organisms using conventional tests and pyrolysis mass spectrometry. Zentrabl Bakteriol 285:195–203
    [Google Scholar]
  12. Pot B., Vandamme P., Kersters K. 1994; Analysis of electrophoretic whole-organism protein fingerprints. In Modern Microbial Methods: Chemical Methods in Prokaryotic Systematics pp. 493–521 Edited by Good-fellow M., O’Donnell A. G. Wiley; Chichester:
    [Google Scholar]
  13. Schleifer K.-H., Ehrmann M., Krusch U., Neve H. 1991; Revival of the species Streptococcus thermophilus {ex Orla-Jensen, 1919) nom. rev. Syst Appl Microbiol 14:386–388
    [Google Scholar]
  14. Truper H. G., De'Clari L. 1997; Taxonomic note: necessary correction of specific epithets formed as substantives (nouns) ‘in apposition’. Int J Syst Bacteriol 47:908–909
    [Google Scholar]
  15. Vandamme P., Devriese L. A., Pot B., Kersters K., Melin P. 1997; Streptococcus difficile is a nonhemolytic group B, type lb streptococcus. Int J Syst Bacteriol 47:81–85
    [Google Scholar]
  16. Vandamme P., Pot B., Falsen E., Kersters K., Devriese L. A. 1996; Taxonomic study of Lancefield streptococcal groups C, G, and L Streptococcus dysgalactiae) and proposal of S. dysgalactiae subsp. equisimiliss subsp. nov. Int J Syst Bacteriol 46:774–781
    [Google Scholar]
  17. Vandamme P., Pot B., Gillis M., De Vos P., Kersters K., Swings J. 1996; Polyphasic taxonomy, a consensus approach to bacterial classification. Microbiol Rev 60:407–438
    [Google Scholar]
  18. Wayne L. G. 1994; Actions of the Judicial Commission of the International Committee on Systematic Bacteriology on requests for opinions published between January 1985 and July 1993. Int J Syst Bacteriol 44:177–178
    [Google Scholar]
  19. Whiley R. A., Russell R. R. B., Hardie J. M., Beighton D. 1988; Streptococcus downei sp. nov. for strains previously described as Streptococcus mutans serotype h. Int J Syst Bacteriol 38:25–29
    [Google Scholar]
  20. Whiley R. A., Beighton D. 1991; Emended descriptions and recognition of Streptococcus constellatus, Streptococcus intermedius and Streptococcus anginosus as distinct species. Int J Syst Bacteriol 41:1–5
    [Google Scholar]
  21. Whiley R. A., Fraser H. Y., Douglas C. W. I., Hardie J., M„ Williams A. M., Collins M. D. 1990; Streptococcus para-sanguis sp. nov., an atypical viridans Streptococcus from human clinical specimens. FEMS Microbiol Lett 68:115–122
    [Google Scholar]
  22. Whiley R. A., Hardie J. M. 1988; Streptococcus vestibularis sp. nov. from the human oral cavity. Int J Syst Bacteriol 38:335–339
    [Google Scholar]
  23. Whiley R. A., Hardie J. M. 1989; DNA-DNA hybridization studies and phenotypic characteristics of strains within the ‘'Streptococcus millerV group. J Gen Microbiol 135:2623–2633
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-48-1-117
Loading
/content/journal/ijsem/10.1099/00207713-48-1-117
Loading

Data & Media loading...

Most cited Most Cited RSS feed