1887

Abstract

Abstract

Eight strains of a previously undescribed coryneform bacterium had been isolated from human clinical material over a 5-year period. Colonies of the unknown coryneform bacterium had an unusual appearance as they were slightly yellowish and very mucoid. Biochemical and chemotaxonomic characterization revealed that the unknown coryneform bacterium belonged to the genus It could be readily differentiated from all previously described species. Electron microscopy demonstrated the production of an extracellular substance causing connecting filaments between cells as a morphological correlate to the mucoid colonies. Comparative 16S rRNA gene sequence analysis revealed that the unknown coryneform bacterium represented a new subline within the genus for which the name sp. nov. is proposed. The type strain is CCUG 36878 (= DSM 44265 = CIP 105129).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-47-4-952
1997-10-01
2024-04-24
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/47/4/ijs-47-4-952.html?itemId=/content/journal/ijsem/10.1099/00207713-47-4-952&mimeType=html&fmt=ahah

References

  1. API System 1989 API CORYNE analytical profile index, 1st ed.. API System, La-Balme-les-Grottes; France:
    [Google Scholar]
  2. Bayston R., Compton C., Richards K. 1994; Production of extracellular slime by coryneforms colonizing hydrocephalus shunts. J. Clin. Microbiol. 32:1705–1709
    [Google Scholar]
  3. Bernard K. A., Bellèfeuille M., Ewan E. P. 1991; Cellular fatty acid composition as an adjunct to the identification of asporogenous, aerobic, gram-positive rods. J. Clin. Microbiol. 29:83–89
    [Google Scholar]
  4. Collins M. D., Cummins C. S. 1986; Genus Corynebacterium. 1266–1276 Sneath P. H. A., Mair N. S., Sharpe M. E., Holt J. G. Bergey’s manual of systematic bacteriology 2 The Williams & Wilkins Co.; Baltimore, Md.:
    [Google Scholar]
  5. Devereux J., Haeberli P., Smithies D. 1984; A comprehensive set of sequence analysis programmes for the VAX. Nucleic Acids Res. 12:387–395
    [Google Scholar]
  6. Felsenstein J. 1989; PHYLIP—phylogeny inference package (version 3.2). Cladistics 5:164–166
    [Google Scholar]
  7. Funke G., Alvarez N., Pascual C., Falsen E., Akervall E., Sabbe L., Schouls L., Weiss N., Collins M. D. 1997; Actinomyces europaeus sp. nov., isolated from human clinical specimens. Int. J. Syst. Bacteriol. 47:687–692
    [Google Scholar]
  8. Funke G., Martinetti Lucchini G., Pfyπer G. E., Marchiani M., von Graevenitz A. 1993; Characteristics of CDC group 1 and group 1-like coryneform bacteria isolated from clinical specimens. J. Clin. Microbiol. 31:2907–2912
    [Google Scholar]
  9. Funke G., Pascual Ramos C., Collins M. D. 1997; Corynebacterium coyleae sp. nov., isolated from human clinical specimens. Int. J. Syst. Bacteriol. 47:92–96
    [Google Scholar]
  10. Funke G., Pünter V., von Graevenitz A. 1996; Antimicrobial susceptibility patterns of some recently defined coryneform bacteria. Antimicrob. Agents Chemother. 40:2874–2878
    [Google Scholar]
  11. Funke G., von Graevenitz A., Clarridge J. E. III, Bernard K. A. 1997; Clinical microbiology of coryneform bacteria. Clin. Microbiol. Rev. 10:125–159
    [Google Scholar]
  12. Garrison R. G., Mirikitani F. K., Lane J. W. 1983; Fine structural studies of Rhodococcus species. Microbios 36:183–190
    [Google Scholar]
  13. Goodfellow M. 1986; Genus Rhodococcus. 1472–1481 Sneath P. H. A., Mair N. S., Sharpe M. E., Holt J. G. Bergey’s manual of systematic bacteriology 2 The Williams & Wilkins Co.; Baltimore, Md.:
    [Google Scholar]
  14. Goodfellow M. 1992; The family Nocardiaceae. 1188–1213 Balows A., Trüper H. G., Dworkin M., Harder W., Schleifer K. H. The prokaryotes 2 Springer-Verlag; New York, N.Y.:
    [Google Scholar]
  15. Hutson R. A., Thompson D. E., Collins M. D. 1993; Genetic interrelationships of saccharolytic Clostridium botulinum types B, E and F and related Clostridia as revealed by small-subunit rRNA gene sequences. FEMS Microbiol. Lett. 108:103–110
    [Google Scholar]
  16. Leitch R. A., Richards J. C. 1990; Structural analysis of the specific capsular polysaccharide of Rhodococcus equi serotype 1. Biochem. Cell Biol. 68:778–789
    [Google Scholar]
  17. McNeil M. M., Brown J. M. 1994; The medically important aerobic actinomycetes: epidemiology and microbiology. Clin. Microbiol. Rev. 7:357–417
    [Google Scholar]
  18. Mutimer M. D., Woolcock J. B. 1982; API ZYM for identification of Corynebacterium equi. Zentralbl. Bakteriol. Parasitenkd. Infektionskr. Hyg. Abt. 1 Orig. Reihe C 3:410–415
    [Google Scholar]
  19. National Committee for Clinical Laboratory Standards 1997 Minimum inhibitory concentration (MIC) interpretive standards (µg/ml) for organisms other than Haemophilus spp., Neisseria gonorrhoeae, and Streptococcus spp. NCCLS document M7-A4 National Committee for Clinical Laboratory Standards; Wayne, Pa.:
    [Google Scholar]
  20. Pascual C., Lawson P. A., Farrow J. A. E., Gimenez M. Navarro, Collins M. D. 1995; Phylogenetic analysis of the genus Corynebacterium based on 16S rRNA gene sequences. Int. J. Syst. Bacteriol. 45:724–728
    [Google Scholar]
  21. Ruimy R., Riegel P., Boiron P., Monteil H., Christen R. 1995; Phylogeny of the genus Corynebacterium deduced from analyses of small-subunit ribosomal DNA sequences. Int. J. Syst. Bacteriol. 45:740–746
    [Google Scholar]
  22. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4:406–425
    [Google Scholar]
  23. Uchida K., Aida K. 1984; An improved method for the glycolate test for simple identification of the acyl type of bacterial cell walls. J. Gen. Appl. Microbiol. 30:131–134
    [Google Scholar]
  24. von Graevenitz A., Funke G. 1996; An identification scheme for rapidly and aerobically growing gram-positive rods. Zentralbl. Bakteriol. Parasitenkd. Infektionskr. Hyg. Abt. 1 Orig. 284:246–254
    [Google Scholar]
  25. von Graevenitz A., Osterhout G., Dick J. 1991; Grouping of some clinically relevant gram-positive rods by automated fatty acid analysis. APMIS 99:147–154
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-47-4-952
Loading
/content/journal/ijsem/10.1099/00207713-47-4-952
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error