1887

Abstract

In an attempt to characterize an unusual mycobacterial strain isolated from a 2-year-old Somali patient with lymphadenitis, we applied various molecular methods not previously used for the taxonomic classification of mycobacteria. This isolate, designated So93, did not differ from in the biochemical tests and in its 16S rRNA sequence, but produced smooth and glossy colonies, which is highly exceptional for this species. This smooth phenotype was unstable and switched nonreversibly to a rough colony morphology with a low frequency. The two colony types were equally virulent for the guinea pig, exhibiting characteristic tuberculous disease. Both morphotypes had shorter generation times than the reference laboratory strain H37Rv and clinical isolates of and . Furthermore, the So93 isolate differed from all complex strains described thus far by having only a single copy of insertion sequence IS, an unusual composition of the direct repeat cluster, and a characteristic phenolic glycolipid and lipooligosaccharide. This glycolipid had previously been observed only in a smooth isolate of obtained in 1969 by Canetti in France. Analysis of the Canetti strain showed that it shared virtually all genetic properties characteristic of So93, distinguishing these two strains from the known complex taxa, , and . The natural reservoir, host range, and mode of transmission of the group of bacteria described in this paper are presently unknown. This study, partly based on not previously used molecular criteria, supports the idea that the established members within the complex and the newly described Canetti grouping should be regarded as a single species, which likely will be designated “”.

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1997-10-01
2022-07-04
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References

  1. Baess I. 1979; Deoxyribonucleic acid relatedness among species of slowly-growing mycobacteria. Acta Pathol. Microbiol. Scand. 87:221–226
    [Google Scholar]
  2. Beckers B., Lang H. R. M., Schimke D., Lammers A. 1985; Evaluation of a bioluminescence assay for rapid antimicrobial susceptibility testing of mycobacteria. Eur. J. Clin. Microbiol. 4:556–561
    [Google Scholar]
  3. Belisle J. T., Brennan P. J. 1989; Chemical basis of rough and smooth variation in mycobacteria. J. Bacteriol. 171:3465–3470
    [Google Scholar]
  4. Belisle J. T., Brennan P. J. 1994; Molecular basis of colony morphology in Mycobacterium avium. Res. Microbiol. 145:237–242
    [Google Scholar]
  5. Belisle J. T., Pascopella L., Inamine J. M., Brennan P. J., Jacobs W. R. Jr. 1991; Isolation and expression of a gene cluster responsible for biosynthesis of the glycopeptidolipid antigens of M. avium. J. Bacteriol. 173:6991–6997
    [Google Scholar]
  6. Bergmans A. M. C., Groothedde J., Schellekens J. F. P., van Embden J. D. A., Ossewaarde J. M., Schouls L. M. 1995; A comparative study on the etiology of cat scratch disease: PCR detection of Bartonella (formerly Rochalimea) and Afipia felis DNA, serology and skin testing. J. Infect. Dis. 171:916–923
    [Google Scholar]
  7. Besra G. S., Bolton R. C., McNeil M. R., Ridell M., Simpson K. E., Glushka J., van Halbeek H., Brennan P. J., Minnikin D. E. 1992; Structural elucidation of a novel family of acyltrehaloses from Mycobacterium tuberculosis. Biochemistry 31:9832–9837
    [Google Scholar]
  8. Blum H., Beir H., Gross H. J. 1987; Improved silver staining of proteins, RNA and DNA. Electrophoresis 8:93–99
    [Google Scholar]
  9. Bunschoten A., Schouls L., Top J., van Soolingen D. Unpublished observations
  10. Calmette A., Negre L., Bouquet A. 1921; Essais de vaccination du lapin et du cobaye contre l’infection tuberculeuse. Ann. Inst. Pasteur (Paris) 36:625–631
    [Google Scholar]
  11. Cole S. 1997 Personal communication
  12. Daffe M., McNeil M., Brennan P. J. 1991; Novel type-specific lipooli-gosaccharides from Mycobacterium tuberculosis. Biochemistry 30:378–388
    [Google Scholar]
  13. Daffe M., Lacave C., Laneelle M.-A., Laneelle G. 1987; Structure of the major triglycosyl phenol-phthiocerol of Mycobacterium tuberculosis (strain Canetti). Eur. J. Biochem. 167:155–160
    [Google Scholar]
  14. Davis E. O., Sedgwick S. G., Colston M. J. 1991; Novel structure of the recA locus of Mycobacterium tuberculosis implies processing of the gene product. J. Bacteriol. 173:5653–5662
    [Google Scholar]
  15. Feizabadi M. M., Robertson I. D., Cousins D. V., Hampson D. J. 1996; Genomic analysis of Mycobacterium bovis and other members of the Mycobacterium tuberculosis complex by isoenzyme analysis and pulsed-field gel electrophoresis. J. Clin. Microbiol. 34:1136–1142
    [Google Scholar]
  16. Gangadharam P. R. J. 1984 Drug resistance in mycobacteria. CRC Press, Inc.; Boca Raton, Fla.:
    [Google Scholar]
  17. Hermans P. W. M., Sluijter M., Hoogenboezem T., Heersma H., van Beikum A, de Groot R. 1995; Comparative study of five different DNA fingerprint techniques for molecular typing of Streptococcus pneumoniae strains. J. Clin. Microbiol. 33:1606–1612
    [Google Scholar]
  18. Hermans P. W. M., van Soolingen D., Bik E. M., de Haas P. E. W., Dale J. W., van Embden J. D. A. 1991; Insertion element ÏS987 from Mycobacterium bovis BCG is located in a hot-spot integration region for insertion elements in Mycobacterium tuberculosis complex strains. Infect. Immun. 59:2695–2705
    [Google Scholar]
  19. Hermans P. W. M., van Soolingen D., van Embden J. D. A. 1992; Characterization of a major polymorphic tandem repeat in Mycobacterium tuberculosis and its potential use in the epidemiology of Mycobacterium kansasii and Mycobacterium gordonae. J. Bacteriol. 174:4157–4165
    [Google Scholar]
  20. Kamerbeek J., Schouls L., Kolk A., van Agterveld M., van Soolingen D., Kuijper S., Bunschoten A., Molhuizen H., Shaw R., Goyal M., van Embden J. D. A. 1997; Simultaneous detection and strain differentiation of Mycobacterium tuberculosis for diagnosis and epidemiology. J. Clin. Microbiol. 35:907–914
    [Google Scholar]
  21. Kapur V., Whittam T. S., Musser J. M. 1994; Is Mycobacterium tuberculosis 15,000 years old?. J. Infect. Dis. 170:1348–1349
    [Google Scholar]
  22. Kirschner P., Springer B., Vogel U., Meier A., Wrede A., Kiekenbeck M., Bange F.-C., Bôttger E. C. 1993; Genotypic identification of mycobacteria by nucleic acid sequence determination: report of a 2-year experience in a clinical laboratory. J. Clin. Microbiol. 31:2882–2889
    [Google Scholar]
  23. Lévy-Frébault V., Portaels F. 1992; Proposed minimal standards for the genus Mycobacterium and for description of new slowly growing Mycohactenum species. Int. J. Syst. Bacteriol. 42:315–323
    [Google Scholar]
  24. Meylan P. R., Richman D. D., Kornbluth R. S. 1990; Characterization and growth in human macrophages of Mycobacterium avium complex strains isolated from the blood of patients with acquired immunodeficiency syndrome. Infect. Immun. 58:2564–2568
    [Google Scholar]
  25. Mitchison D. A., Bhatia A. L., Radhakrishna S., Selkon J. B., Subbaiah T. V., Wallace J. G. 1961; The virulence in the guinea pig of tubercle bacilli isolated before treatment from South Indian patients with pulmonary tuberculosis. Homogeneity of the investigation and a critique of the virulence test. Bull. W.H.O. 25:285–312
    [Google Scholar]
  26. Orita M., Iwahana H., Kanazawa H., Hayashi K., Sekiya T. 1989; Detection of polymorphisms of human DNA by gel electrophoresis as singlestrand conformation polymorphisms. Proc. Natl. Acad. Sci. USA 86:2766–2770
    [Google Scholar]
  27. Petroff S. A. 1927; Microbic dissociation: the tubercle bacillus. Proc. Soc. Exp. Biol. Med. 24:632
    [Google Scholar]
  28. Petroff S. A., Steenken W. Jr. 1930; Biological studies of the tubercle bacillus. J. Exp. Med. 51:831–845
    [Google Scholar]
  29. Rastogi N., Levy-Frebault V., Blom-Potar M., David H. L. 1989; Ability of smooth and rough variants of Mycobacterium avium and M. intracellulare to multiply and survive intracellularly: role of C-mycosides. Zentralbl. Bakteriol. Hyg. A 270:345–360
    [Google Scholar]
  30. Romesburg H. C. 1990 Cluster analysis for researchers. R. E. Krieger Publishing Co.; Malabar, Fla.:
    [Google Scholar]
  31. Ross C., Raios K., Jackson K., Dwyer B. 1992; Molecular cloning of a highly repeated DNA element from Mycobacterium tuberculosis and its use as an epidemiological tool. J. Clin. Microbiol. 30:942–946
    [Google Scholar]
  32. Runyon E. H. 1970; Identification of mycobacterial pathogens utilizing colony characteristics. Am. J. Clin. Pathol. 54:578–586
    [Google Scholar]
  33. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74:5463–5467
    [Google Scholar]
  34. Schaefer W. B., Davis C. L., Cohn M. L. 1970; Pathogenicity of transparent, opaque, and rough variants of Mycobacterium avium in chickens and mice. Am. Rev. Respir. Dis. 102:499–506
    [Google Scholar]
  35. Silva M. T., Macedo P. M. 1984; Ultrastructural characterization of normal and damaged membranes of Mycobacterium leprae and of cultivable mycobacteria. J. Gen. Microbiol. 130:369–380
    [Google Scholar]
  36. Steenken W. Jr., Gardner L. U. 1946; History of H37 strain of tubercle bacillus. Am. Rev. Tuberc. 54:62–66
    [Google Scholar]
  37. Thiery J. P., Rambourg A. 1974; Cytochimie des polysaccharides. J. Microsc. 21:225–232
    [Google Scholar]
  38. Thoen C. O., Karlson A. G., Himes E. M. 1984 Disease in domestic and feral animals. 1209–1236 Kubica G. O., Wayne L. G.ed The mycobacteria: a sourcebook, part B Marcel Dekker; New York, N.Y.:
    [Google Scholar]
  39. Tsukamura M., Mizuno S., Toyama H. 1985; Taxonomic studies on the Mycobacterium tuberculosis series. Microbiol. Immiinol. 29:285–299
    [Google Scholar]
  40. van Soolingen D., de Haas P. E. W., Blumenthal R. M., Kremer K., Sluijter M., Pijnenburg J. E. M., Schouls L. M., Thole J. E. R., Dessens-Kroon M. W. G., van Embden J. D. A., Hermans P. W. M. 1996; Host-mediated modification of Pvull restriction in Mycobacterium tuberculosis. J. Bacteriol. 178:78–84
    [Google Scholar]
  41. van Soolingen D., de Haas P. E. W., Hermans P. W. M., vail Embden J. D. A. 1994; DNA fingerprinting of Mycobacterium tuberculosis. Methods Enzymol. 235:196–205
    [Google Scholar]
  42. van Soolingen D., de Haas P. E. W., Hermans P. W. M., Groenen P. M. A., van Embden J. D. A. 1993; Comparison of various repetitive DNA elements as genetic markers for strain differentiation and epidemiology of Mycobacterium tuberculosis. J. Clin. Microbiol. 31:1987–1995
    [Google Scholar]
  43. van Soolingen D., Hermans P. W. M., de Haas P. E. W., Soli D. R., van Embden J. D. A. 1991; The occurrence and stability of insertion sequences in Mycobacterium tuberculosis complex strains: evaluation of an insertion sequence-dependent DNA polymorphism as a tool in the epidemiology of tuberculosis. J. Clin. Microbiol. 29:2578–2586
    [Google Scholar]
  44. van Soolingen D., Hermans P. W. M., de Haas P. E. W., van Embden J. D. A. 1992; Insertion element IS70<8I-associated restriction fragment length polymorphisms in Mycobacterium tuberculosis complex species: a reliable tool for recognizing Mycobacterium bovis BCG. J. Clin Microbiol. 30:1772–1777
    [Google Scholar]
  45. van Soolingen D., Qian L., de Haas P. E. W., Douglas J. T., Traore H., Portaels F., Qing H. Z., Enkhsaikan D., Nymadawa P., van Embden J. D. A. 1995; Predominance of a single genotype of Mycobacterium tuberculosis in countries of East Asia. J. Clin. Microbiol. 33:3234–3238
    [Google Scholar]
  46. Van Steenbergen T. J. M., CollomS S. D., Hermans P. W. M., de Graaff J., Plasterk R. H. A. 1995; Genomic DNA fingerprinting by restriction fragment end labeling (RFEL). Proc. Natl. Acad. Sci. USA 92:5572–5576
    [Google Scholar]
  47. Wayne L. G., Kubica G. P. 1986 The mycobacteria. 1435–1457 Sneath P. H. A., Holt J. G.ed Bergey’s manual of systematic bacteriology 2 The Williams & Wilkins Co.; Baltimore, Md.:
    [Google Scholar]
  48. Wells A. Q. 1945 The murine type of tubercle bacillus. Medical Research Council, Sir William Dunn School of Pathology University of Oxford; Oxford, United Kingdom: Special report series no. 259
    [Google Scholar]
  49. Winn W. A., Petroff S. A. 1933; A new conception of the pathology of experimental avian tuberculosis with special reference to the disease produced by dissociated variants. J. Exp. Med. 57:239–264
    [Google Scholar]
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