1887

Abstract

Organisms with characteristics typical of mycoplasmas were isolated from the preputial smegma of Afghan picas (). The results of growth inhibition tests, metabolic inhibition tests, and immunobinding assays showed that the isolated strains were identical and that they were distinct from previously described , and species. These organisms represent a new species, for which the name is proposed. ferments glucose, does not hydrolyze arginine or urea, reduces tetrazolium chloride, possesses phosphatase activity, does not digest gelatin or casein, and does not produce films or spots. It lyses sheep erythrocytes and does not adsorb sheep, rabbit, or horse erythrocytes. Cholesterol or serum is required for growth. The growth temperature is 37°C. The guanine-plus-cytosine content of the DNA is 23.0 ± 1.0 mol%. The type strain is 12MS (= ATCC 700289).

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1997-10-01
2024-06-14
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References

  1. Aluotto B. B., Wittier R. G., Williams C. O., Faber J. E. 1970; Standardized bacteriologic techniques for the characterization of Mycoplasma species. Int. J. Syst. Bacteriol. 20:35–58
    [Google Scholar]
  2. Bradbury J. M. 1977; Rapid biochemical tests for characterization of Mycoplasmatales. J. Clin. Microbiol. 5:70–76
    [Google Scholar]
  3. Cassell G. H., Hill A. 1979 Murine and other small-animal mycoplasmas. 235–273 Tully J. G., Whitcomb R. F.ed The mycoplasmas 2 Academic Press, Inc.; New York, N.Y.:
    [Google Scholar]
  4. Clyde W. A. Jr. 1964; Mycoplasma species identification based upon growth inhibition by specific antisera. J. Immunol. 92:958–965
    [Google Scholar]
  5. Deeb B. J., Kenny G. E. 1967; Characterization of Mycoplasma pulmonis variants isolated from rabbits. J. Bacteriol. 93:1416–1429
    [Google Scholar]
  6. Edward D. G. 1971; Determination of sterol requirement for Mycoplasmatales. J. Gen. Microbiol. 69:205–210
    [Google Scholar]
  7. Edward D. G., Fitzgerald W. A. 1951; Cholesterol in the growth of organisms of the pleuropneumonia group. J. Gen. Microbiol. 5:576–586
    [Google Scholar]
  8. Hill A. C. 1983; Mycoplasma cricetuli, a new species from the conjunctivas of Chinese hamsters. Int. J. Syst. Bacteriol. 33:113–117
    [Google Scholar]
  9. Hill A C. 1983; Mycoplasma collis, a new species isolated from rats and mice. Int. J. Syst. Bacteriol. 33:847–851
    [Google Scholar]
  10. Hill A. C. 1984; Mycoplasma cavipharyngis, a new species from the nasopharynx of guinea pigs. J. Gen. Microbiol. 130:3183–3188
    [Google Scholar]
  11. Ishihara C. 1977; Characterization of mycoplasma isolated from rabbits. Bull. Inst. Immunol. Sci. Hokkaido Univ. 37:29–32
    [Google Scholar]
  12. Kotani H., McGarrity G. J. 1985; Rapid and simple identification of mycoplasmas by immunobinding. J. Immunol. Methods 85:257–267
    [Google Scholar]
  13. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J. Mol. Biol. 5:109–118
    [Google Scholar]
  14. McGarrity G. J., Rose D. L., Kwiatkowski V., Dion A. S., Phillips D. M., Tully J. G. 1983; Mycoplasma muris, a new species from laboratory mice. Int. J. Syst. Bacteriol. 33:350–355
    [Google Scholar]
  15. Morton H. E., Roberts R. J. 1967; Production of antimycoplasma (PPLO) antibodies in rabbits. Proc. Soc. Exp. Biol. Med. 125:538–543
    [Google Scholar]
  16. Sobeslavsky O., Prescott B., Chanock R. M. 1968; Absorption of Mycoplasma pneumoniae to neuraminic acid receptors of various cells and possible role in virulence. J. Bacteriol. 96:695–705
    [Google Scholar]
  17. Subcommittee on the Taxonomy of Mollicutes of the International Committee on Systematic Bacteriology 1995; Revised minimum standards for description of new species of the class Mollicutes (division Tenericutes). Int. J. Syst. Bacteriol. 45:605–612
    [Google Scholar]
  18. Taylor-Robinson D., Purcell R. H., Wong D. C., Chanock R. M. 1966; A color test for the measurement of antibody to certain mycoplasma species based upon the inhibition of acid production. J. Hyg. 64:91–104
    [Google Scholar]
  19. Tully J. G. 1983; Cloning and filtration techniques for mycoplasmas. Methods Mycoplasmol. 1:355–362
    [Google Scholar]
  20. Venable J. H., Coggeshall R. 1965; A simplified lead citrate stain for use in electron microscopy. J. Cell Biol. 25:407
    [Google Scholar]
  21. Williams C. O., Wittier R. G. 1971; Hydrolysis of esculin and phosphatase production by members of the order Mycoplasmatales which do not require sterol. Int. J. Syst. Bacteriol. 21:73–77
    [Google Scholar]
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