1887

Abstract

A group of -like strains (designated group S14), isolated from the feces of healthy people, was characterized by biochemical tests, fatty acid analysis, cell wall murein analysis, and 16S rDNA analysis. Our results indicate that group S14 is phylogenetically a member of the subphylum of the gram-positive bacteria. Despite a phenotypic resemblance to the genus , group S14 was shown to be phylogenetically distantly related to the type species of the genus, Group S14 showed a specific phylogenetic association with Group S14 resembled in possessing the uncommon type B cell wall murein. Structural analyses, however, revealed the presence of a previously unknown B1δ (-Ala)--Glu-Gly--Lys murein type. Based on a 16S rRNA sequence divergence of greater than 10% with and the presence of a unique murein type, a new genus, , is proposed for group S14, with one species, Type strain of is ATCC 51649.

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1997-10-01
2024-03-28
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References

  1. Ahrné S., Stenstrôm I.-M., Jensen N. E., Petterson B., Uhlén M., Molin G. 1995; Classification of Erysipelothrix strains on the basis of restriction fragment length polymorphisms. Int. J. Syst. Bacteriol. 45:382–385
    [Google Scholar]
  2. Collins M. D., Lawson P. A., Willems A., Cordoba J. J., Fernandez-Garayzabal J., Garcia P., Cai J., Hippe H., Farrow J. A. E. 1994; The phylogeny of the genus Clostridium: proposal of five new genera and eleven new species combinations. Int. J. Syst. Bacteriol. 44:812–826
    [Google Scholar]
  3. Devereux J., Haeberli P., Smithies D. 1984; A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res. 12:387–395
    [Google Scholar]
  4. Felsenstein J. 1989; PHYLIP–phylogeny inference package (version 3.2). Cladistics 5:164–166
    [Google Scholar]
  5. Holdeman L. V., Cato E. P., Moore W. E. C.ed 1977 Anaerobe laboratory manual. Virginia Polytechnic Institute and State University; Blacksburg.:
    [Google Scholar]
  6. Johnson J. L., Moore L. V. H., Kaneko B., Moore W. E. C. 1990; Actinomyces georgiae sp. nov., Actinomyces gerencseriae sp. nov., designation of two genospecies of Actinomyces naeslundii, and inclusion of A. naeslundii serotypes II and III and Actinomyces viscosus serotype II in A. naeslundii genospecies 2. Int. J. Syst. Bacteriol. 40:272–286
    [Google Scholar]
  7. Jones D. 1986 Genus Erysipelothrix Rosenbach 1909. 1245–1249 Sneath P. H. A., Mair N. S., Sharpe M. E., Holt J. G.ed Bergey’s manual of systematic bacteriology 2 The Williams & Wilkins Co.; Baltimore, Md.:
    [Google Scholar]
  8. Lawson P. A., Gharbia S. E., Shah H. N., Clark D. R. 1989; Recognition of Fusobacterium nucleatum subgroups Fn-1, Fn-2 and Fn-3 by ribosomal RNA gene restriction patterns. FEMS Microbiol. Lett. 65:41–46
    [Google Scholar]
  9. Moore W. E. C., Holdeman L. V. 1974; Human fecal flora: the normal flora of 20 Japanese-Hawaiians. Appl. Microbiol. 27:961–979
    [Google Scholar]
  10. Moore W. E. C., Holdeman Moore L. V. 1986 Genus Eubacterium Prévôt 1938. 1353–1373 Sneath P. H. A., Mair N. S., Sharpe M. E., Holt J. G.ed Bergey’s manual of systematic bacteriology 2 The Williams & Wilkins Co.; Baltimore, Md.:
    [Google Scholar]
  11. Moore W. E. C., Johnson J. L., Holdeman L. V. 1976; Emendation of Bacteroidaceae and Butyrivibrio and descriptions of Desulfomonas gen. nov. and ten new species in the genera Desulfomonas; Butyrivibrio, Eubacterium, Clostridium, and Ruminococcus. Int. J. Syst. Bacteriol. 26:238–252
    [Google Scholar]
  12. Morris G. N., Winter J., Cato E. P., Ritchie A. E., Bokkenheuser V. D. 1986; Eubacterium desmolans sp. nov., a steroid desmolase-producing species from cat fecal flora. Int. J. Syst. Bacteriol. 36:183–186
    [Google Scholar]
  13. Mountfort D. P., Grant W. D., Morgan H., Rainey F. A., Stackebrandt E. 1993; Isolation and characterization of an obligately anaerobic, pectinolytic member of the genus Eubacterium from mullet gut. Arch. Microbiol. 159:289–295
    [Google Scholar]
  14. Schleifer K. H., Kandler O. 1972; Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol. Rev. 36:407–477
    [Google Scholar]
  15. Takahashi T., Fujisawa T., Tamura Y., Suzuki S., Muramatsu M., Sawada T., Benno Y., Mitsuoka T. 1992; DNA relatedness among Erysipelothrix rhusiopathiae strains representing all twenty-three serovars and Erysipelothrix tonsillarum. Int. J. Syst. Bacteriol. 42:469–473
    [Google Scholar]
  16. Takamine F., Imamura T. 1995; Isolation and characterization of bile acid 7-hydroxylating bacteria from human feces. Microbiol. Immunol. 39:11–18
    [Google Scholar]
  17. Tanner R. S., Stackebrandt E., Fox G. E., Woese C. R. 1981; A phylogenetic analysis of Acetobacterium woodii, Clostridium barkeri, Clostridium butyricum, Clostridium lituseburense, Eubacterium limosum, and Eubacterium tenue. Curr. Microbiol. 5:35–38
    [Google Scholar]
  18. White B. A., Lipsky R. L., Fricke R. J., Hylemon P. B. 1980; Bile acid induction specificity of 7a-hydroxylase activity in an intestinal Eubacterium species. Steroids 35:103–109
    [Google Scholar]
  19. Wilkins T. D., Fulghum R. S., Wilkins J. H. 1974; Eubacterium plexicaudatum sp. nov., an anaerobic bacterium with a subpolar tuft of flagella, isolated from a mouse cecum. Int. J. Syst. Bacteriol. 24:408–411
    [Google Scholar]
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