1887

Abstract

We performed an integrated genotypic and phenotypic analysis of 128 strains of the genera Burkholderia, , and in order to study the taxonomic structure of and its relationships with other species. Our data show that presumed strains isolated from cystic fibrosis patients belong to at least five distinct genomic species, one of which was identified as This group of five phenotypically similar species is referred to as the complex. The name is proposed for one of these genomic species, which was formerly referred to as genomovar II; the remaining groups are referred to as genomovars I, III, and IV, pending additional differential phenotypic tests. The role and pathogenic potential of each of these taxa, particularly in view of the potentially fatal infections in cystic fibrosis patients, need further evaluation. The data presented also demonstrate that and should be reclassified as species.

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1997-10-01
2022-05-25
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References

  1. Anonymous 1992; Pseudomonas cepacia–more than a harmless commensal?. Lancet 339:1385–1386 Editorial
    [Google Scholar]
  2. Bevivino A., Tabacchioni S., Chiarini L., Carusi M. V., Del Gallo M., Visca P. 1994; Phenotypic comparison between rhizosphere and clinical isolates of Burkholderia cepacia. Microbiology 140:1069–1077
    [Google Scholar]
  3. Butler S. L., Doherty C. J., Hughes J. E., Nelson J. W., Govan J. R. W. 1995; Burkholderia cepacia and cystic fibrosis: do natural environments present a potential hazard? J. Clin. Microbiol. 33:1001–1004
    [Google Scholar]
  4. Cheng H.-P., Lessie T. 1994; Multiple replicons constituting the genome of Pseudomonas cepacia 17616. J. Bacteriol. 176:4034–4042
    [Google Scholar]
  5. Christenson J. C., Welch D. F., Mukwaya G., Muszynski M. J., Weaver R. E., Brenner D. J. 1989; Recovery of Pseudomonas gladioli from respiratory tract specimens of patients with cystic fibrosis. J. Clin. Microbiol. 27:270–273
    [Google Scholar]
  6. Costas M. 1992 Classification, identification, and typing of bacteria by the analysis of their one-dimensional polyacrylamide gel electrophoretic protein patterns. 351–408 Chambrach A., Dunn M. J., Radola B. J.ed Advances in electrophoresis 5 VCH Verlagsgesellschaft; Weinheim, Germany.:
    [Google Scholar]
  7. De Ley J. 1970; Reexamination of the association between melting point, buoyant density, and chemical base composition of deoxyribonucleic acid. J. Bacteriol. 101:738–754
    [Google Scholar]
  8. De Ley J., Cattoir H., Reynaerts A. 1970; The quantitative measurement of DNA hybridization from renaturation rates. Eur. J. Biochem. 12:133–142
    [Google Scholar]
  9. De Vos P., De Ley J. 1983; Intra- and intergeneric similarities of Pseudomonas and Xanthomonas ribosomal ribonucleic acid cistrons. Int. J. Syst. Bacteriol. 33:487–509
    [Google Scholar]
  10. Gillis M., Van T. V., Bardin R., Goor M., Hebbar P., Willems A., Segers P., Kersters K., Heulin T., Fernandez M. P. 1995; Polyphasic taxonomy in the genus Burkholderia leading to an emended description of the genus and proposition of Burkholderia vietnamiensis sp. nov. for N2-fixing isolates from rice in Vietnam. Int. J. Syst. Bacteriol. 45:274–289
    [Google Scholar]
  11. Govan J. R. W., Brown P. H., Maddison J., Doherty C. J., Nelson J. W., Dodd M., Greening A. P., Webb A. K. 1993; Evidence for transmission of Pseudomonas cepacia by social contact in cystic fibrosis. Lancet 342:15–19
    [Google Scholar]
  12. Govan J. R. W., Deretic V. 1996; Microbial pathogenesis in cystic fibrosis: mucoid Pseudomonas aeruginosa and Burkholderia cepacia. Microbiol. Rev. 60:539–574
    [Google Scholar]
  13. Govan J. R. W., Hughes J., Vandamme P. 1996; Burkholderia cepacia·. medical, taxonomic and ecological issues. J. Med. Microbiol. 45:395–407
    [Google Scholar]
  14. Holmes B., Lapage S. P., Malnick H. 1975; Strains of Pseudomonas putrefaciens from clinical material. J. Clin. Pathol. 28:149–155
    [Google Scholar]
  15. Isles A., Maclusky I., Corey M., Gold R., Prober C., Fleming P., Levison H. 1984; Pseudomonas cepacia infection in cystic fibrosis: an emerging problem. J. Pediatr. 104:206–210
    [Google Scholar]
  16. Kersters K., Ludwig W., Vancanneyt M., De Vos P., Gillis M., Schleifer K.-H. 1996; Recent changes in the classification of the pseudomonads: an overview. Syst. Appl. Microbiol. 19:465–477
    [Google Scholar]
  17. Kiska D. L., Kerr A., Jones M. C., Caracciolo J. A., Eskridge B., Jordan M., Miller S., Hughes D., King N., Gilligan P. 1996; Accuracy of four commercial systems for identification of Burkholderia cepacia and other gram-negative nonfermenting bacilli recovered from patients with cystic fibrosis. J. Clin. Microbiol. 34:886–891
    [Google Scholar]
  18. Larsen G. Y., Stull T. L., Burns J. L. 1993; Marked phenotypic variability in Pseudomonas cepacia isolated from a patient with cystic fibrosis. J. Clin. Microbiol. 31:788–792
    [Google Scholar]
  19. Leff L. G., Kernan R. M., McArthur J. V., Shimkets L. J. 1995; Identification of aquatic Burkholderia (Pseudomonas) cepacia by hybridization with species-specific rRNA gene probes. Appl. Environ. Microbiol. 61:1634–1636
    [Google Scholar]
  20. Lessie T. G., Hendrickson W., Manning B. D., Devereux R. 1996; Genomic complexity and plasticity of Burkholderia cepacia. FEMS Microbiol. Lett. 144:117–128
    [Google Scholar]
  21. Palleroni N. J. 1984 Genus I. Pseudomonas Migula 1894, 237AL (nom. cons, opin. 5, Jud. Comm. 1952, 237). 141–199 Krieg N. R., Holt J. G.ed Bergey’s manual of systematic bacteriology I Williams & Wilkins; Baltimore, Md:
    [Google Scholar]
  22. Palleroni N. J., Holmes B. 1981; Pseudomonas cepacia sp. nov., nom. rev. Int. J. Syst. Bacteriol. 31:479–481
    [Google Scholar]
  23. Palleroni N. J., Kunisawa R., Contopoulou R., Doudoroff M. 1973; Nucleic acid homologies in the genus Pseudomonas. Int. J. Syst. Bacteriol. 23:333–339
    [Google Scholar]
  24. Pitt T. L., Kaufmann M. E., Patel P. S., Benge L. C. A., Gaskin S., Livermore D. M. 1996; Type characterization and antibiotic susceptibility of Burkholderia (Pseudomonas) cepacia isolates from patients with cystic fibrosis in the United Kingdom and the Republic of Ireland. J. Med. Microbiol. 44:203–210
    [Google Scholar]
  25. Pot B., Vandamme P., Kersters K. 1994 Analysis of electrophoretic whole-organism protein fingerprinting. 493–521 Goodfellow M., O’Donnell A. G.ed Modern microbial methods. Chemical methods in bacterial systematics J. Wiley and Sons, Chichester; United Kingdom:
    [Google Scholar]
  26. Revets H., Vandamme P., Van Zeebroeck A., De Boeck K., Struelens M. J., Verhaegen J., Ursi J. P., Verschraegen G., Franckx H., Malfroot A., Dab I., Lauwers S. 1996; Burkholderia (Pseudomonas) cepacia and cystic fibrosis: the epidemiology in Belgium. Acta Clin. Belg. 51:222–230
    [Google Scholar]
  27. Rodley P. D., Romling U., Tummler B. 1995; A physical map of the Burkholderia cepacia type strain. Mol. Microbiol. 17:57–67
    [Google Scholar]
  28. Simpson I. N., Finlay J., Winstanley D. J., Dewhurst N., Nelson J. W., Butler S. L., Govan J. R. W. 1994; Multi-resistance isolates possessing characteristics of both Burkholderia (Pseudomonas) cepacia and Burkholderia gladioli from patients with cystic fibrosis. J. Antimicrob. Chemother. 34:353–361
    [Google Scholar]
  29. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int. J. Syst. Bacteriol. 44:846–849
    [Google Scholar]
  30. Stanier R. Y., Palleroni N. J., Doudoroff M. 1966; The aerobic pseudomonads: a taxonomic study. J. Gen. Microbiol. 43:159–271
    [Google Scholar]
  31. Stead D. E. 1992; Grouping of plant-pathogenic bacteria and some other Pseudomonas spp. by using cellular fatty acid profiles. Int. J. Syst. Bacteriol. 42:281–295
    [Google Scholar]
  32. Sun L., Jiang R.-Z., Steinbach S., Holmes A., Campanelli C., Forstner J., Tan Y., Riley M., Goldstein R. 1995; The emergence of a highly transmissible lineage of Cbl+ Pseudomonas (Burkholderia) cepacia causing CF center epidemics in North America and Britain. Nat. Med. 1:661–666
    [Google Scholar]
  33. Tabacchioni S., Visca P., Chiarini L., Bevivino A., Di Serio C., Fancelli S., Fani R. 1995; Molecular characterization of rhizosphere and clinical isolates of Burkholderia cepacia. Res. Microbiol. 146:531–542
    [Google Scholar]
  34. Tyler S. D., Strathdee C. A., Rozee K. R., Johnson W. M. 1995; Oligonucleotide primers designed to differentiate pathogenic pseudomonads on the basis of the sequencing of genes coding for 16S-23S rRNA internal transcribed spacers. Clin. Diagn. Lab. Immunol. 2:448–453
    [Google Scholar]
  35. Urakami T., Ito-Yoshida C., Araki H., Kijima T., Suzuki K.-I., Komagata K. 1994; Transfer of Pseudomonas plantarii and Pseudomonas glumae to Burkholderia as Burkholderia spp. and description of Burkholderia vandii sp. nov. Int. J. Syst. Bacteriol. 44:235–245
    [Google Scholar]
  36. Ursing J. B., Rosselld-Mora R. A., Garcia-Valdes E., Lalucat J. 1995; Taxonomic note: a pragmatic approach to the nomenclature of phenotypically similar genomic groups. Int. J. Syst. Bacteriol. 45:604
    [Google Scholar]
  37. Vandamme P. 1995; Emerging new “Pseudomonas” species in cystic fibrosis. Presented at the 20th European Cystic Fibrosis ConferenceBrussels, Belgium
    [Google Scholar]
  38. Vandamme P. Unpublished data
  39. Vandamme P., Vancanneyt M., Pot B., Meis L., Hoste B., Dewettinck D., Vlaes L., Van Den Borre C., Higgins R., Hommez J., Kersters K., Butzler J.-P., Goossens H. 1992; Polyphasic taxonomic study of the emended genus Arcobacter with Arcobacter butzleri comb. nov. and Arcobacter skirrowii sp. nov., an aerotolerant bacterium isolated from veterinary specimens. Int. J. Syst. Bacteriol. 42:344–356
    [Google Scholar]
  40. Van Landschoot A., De Ley J. 1983; Intra- and intergeneric similarities of the rRNA cistrons of Alteromonas, Marinomonas (gen. nov.) and some other Gram-negative bacteria. J. Gen. Microbiol. 129:3057–3074
    [Google Scholar]
  41. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler P., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E., Stackebrandt E., Starr M. P., Triiper H. G. 1987; Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int. J. Syst. Bacteriol. 37:463–464
    [Google Scholar]
  42. Whiteford M. L., Wilkinson J. D., McColl J. H., Conlon F. M., Michie J. R., Evans T. J., Paton J. Y. 1995; Outcome of Burkholderia (Pseudomonas) cepacia colonisation in children with cystic fibrosis following a hospital outbreak. Thorax 50:1194–1198
    [Google Scholar]
  43. Yabuuchi E., Kosako Y., Oyaizu H., Yano I., Hotta H., Hashimoto Y., Ezaki T., Arakawa M. 1992; Proposal of Burkholderia gen. nov. and transfer of seven species of the genus Pseudomonas homology group II to the new genus, with the type species Burkholderia cepacia (Palleroni and Holmes 1981) comb. nov. Microbiol. Immunol. 36:1251–1275
    [Google Scholar]
  44. Yabuuchi E., Kosako Y., Yano I., Hotta H., Nishiuchi Y. 1995; Transfer of two Burkholderia and an Alcaligenes species to Ralstonia gen. nov.: proposal of Ralstonia pickettii (Ralston, Palleroni and Doudoroff 1973) comb, nov., Ralstonia solanacearum (Smith 1896) comb. nov. and Ralstonia eutropha (Davis 1969) comb. nov. Microbiol. Immunol. 39:897–904
    [Google Scholar]
  45. Yohalem D. S., Lorbeer J. W. 1994; Multilocus isoenzyme diversity among strains of Pseudomonas cepacia isolated from decayed onions, soils, and clinical sources. Syst. Appl. Microbiol. 17:116–124
    [Google Scholar]
  46. Zhao N., Qu C., Wang E., Chen W. 1995; Phylogenetic evidence for the transfer of Pseudomonas cocovenenans (van Damme et al. 1960) to the genus Burkholderia as Burkholderia cocovenenans (van Damme et al. 1960) comb, nov. Int. J. Syst. Bacteriol. 45:600–603
    [Google Scholar]
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