We examined the taxonomic position of seven isolates, recovered from Flemish and Scottish drinking water production plants and reservoirs, which were previously recognized by numerical analysis of genomic AFLP fingerprints as members of an unknown taxon that most closely resembled the species (DNA hybridization group [HG] 2). The new phenotypic and DNA-DNA hybridization data obtained in this study show that the -like strains constitute a separate species, for which the name sp. nov. is being proposed. The new species exhibited an internal DNA relatedness ranging from 79 to 100% and was 22 to 63% related to the type or reference strains of other spp. The highest DNA binding values were determined with (51 to 63%), followed by sensu stricto (HG1; 50 to 60%) and (HG3; 39 to 55%). Although fingerprints generated by ribotyping and cellular fatty acid analysis often were highly similar, minor differences between the respective fingerprints were of significance for the differentiation of from its closest taxonomic neighbors, HG1, HG2, and HG3. Phenotypically, all seven strains of were positive for acid and gas production from D-glucose and glycerol, growth in KCN broth, arginine dihydrolase, DNase, Voges-Proskauer reaction, and resistance to vibriostatic agent O/129 and ampicillin but displayed negative reactions for production of urease, tryptophan deaminase, ornithine decarboxylase, and lysine decarboxylase (LDC). None of the strains displayed strong hemolytic activity. The lack of D-sucrose fermentation and LDC production and the ability to utilize DL-lactate as the sole energy and carbon source were useful characteristics for the biochemical separation of from Other spp. could be differentiated phenotypically from the new species by at least two features. The chromosomal G+C content of ranges from 57.7 to 59.6 mol%. Strain LMG 17541 is proposed as the type strain.


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