A novel actinomycete was isolated from compost soil and was studied taxonomically and phylogenetically. Cells of this organism were gram positive, not acid fast, nonmotile, nonsporulating, irregular coccoid to short rod shaped, and microaerophilic. The cell wall peptidoglycan contained lysine and was cross-linked via an l-Lys←.-Ser←D-Asp interpeptide bridge. The major menaquinone was MK-8(H4). The polar lipids were phosphatidylinositol, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, and two unknown phospholipids. Mycolic acids were absent The cellular fatty acid profile was complex, with large amounts of saturated and monounsaturated straight-chain acids and smaller amounts of iso and anteiso branched-chain acids. The G+C content of the DNA was 66 mol%. Comparative 16S ribosomal DNA studies revealed that strain HKI0089T represents a novel lineage within Actinobacteria (32) distinct from all previously described genera and most closely related to members of the genera Kytococcus, Dermacoccus, and Dermatophilus of the family Dermatophilaceae. On the basis of our results, we suggest that strain HKI 0089 should be classified in a new genus and species, for which we propose the name Demetria terragena. The type strain and the only strain of the genus and species is HKI 0089 (DSM 11295).
BrosiusJ., PalmerM. L., KennedyP. J., NollerH. F.1978; Complete nucleotide sequence of the 16S ribosomal RNA gene from Escherichia coli. Proc. Natl. Acad. Sci. USA 75:4801–4805
CollinsM. D., JonesD.1980; Lipids in the classification and identification of coryneform bacteria containing peptidoglycans based on 2,4-diaminobutyric acid. J. Appl. Bacteriol. 48:459–470
CollinsM. D., PirouzT., GoodfellowM., MinnikinD. E.1977; Distribution of menaquinones in actinomycètes and corynebacteria. J. Gen. Microbiol. 100:221–230
DSM-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH1993Catalogue of strains, 5th. DSM-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH; Braunschweig, Germany.:
FrankH., RettenmeierA., WeickerH., NicholsonG. J., BayerE.1980; A new gas chromatographic method for determination of amino acid levels in human serum. Clin. Chim. Acta 105:201–211
GledhillW. E., CasidaL. E.Jr.1969; Predominant catalase-negative soil bacteria. III. Agromyces, gen. n, microorganisms intermediary to Actinomyces and Nocardia. Appl. Microbiol. 18:340–349
GordonM. A.1989 Genus Dermatophilus Van Saçeghem 1915. 2409–2410WilliamsS. T., SharpeM. E., HoltJ. G.edBergey’s manual of systematic bacteriology4 The Williams & Wilkins Co; Baltimore, Md.:
GordonR. E, BarnettD. A., HanderhanJ. E., PangC. H.-N.1974; Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. Int. J. Syst. Bacteriol. 24:54–63
Greiner-MaiE., Korn-WendischF., KutznerH. J.1988; Taxonomic revision of the genus Saccharomonospora and description of Saccharomonospora glauca sp. nov. Int. J. Syst. Bacteriol. 38:398–405
GrothI., SchumannP., RaineyF. A., MartinK., SchuetzeB., AugstenK.1997; Bogoriella caseilytica gen. nov, sp. nov, a new alkaliphilic coryneform bacterium from a soda lake in Africa. Int. J. Syst. Bacteriol. 47:788–794
GrothI., SchumannP., WeissN., MartinK., RaineyF. A.1996; Agrococcus jenensis gen. nov, sp. nov, a new genus of actinomycètes with diaminobutyric acid in the cell wall. Int. J. Syst. Bacteriol. 46:234–239
HughR., LeifsonE.1953; The taxonomic significance of fermentative versus oxidative metabolism of carbohydrates by various gram negative bacteria. J. Bacteriol. 66:24–26
KocurM.1986 Genus Micrococcus Cohn 1872. 1004–1008SneathP. H. A., MairN. S., SharpeM. E., G· HoltJ.edBergey’s manual of systematic bacteriology2 The Williams & Wilkins Co; Baltimore, Md.:
LechevalierH. A.1986 Nocardioforms. 1458–1459SneathP. H. A., MairN. S., SharpeM. E., HoltJ. G.edBergey’s manual of systematic bacteriology2 The Williams & Wilkins Co; Baltimore, Md.:
MartinK., SchumannP, RaineyF. A., SchuetzeB., GrothI.1997; Janibacter limosus gen. nov, sp. nov, a new actinomycète with meso-diamin-opimelic acid in the cell wall. Int. J. Syst. Bacteriol. 47:529–534
MinnikinD. E, AlshamaonyL., GoodfellowM.1975; Differentiation of Mycobacterium, Nocardia, and related taxa by thin-layer chromatographic analysis of whole-organism methanolysates. J. Gen. Microbiol. 88:200–204
MinnikinD. E, CollinsM. D., GoodfellowM.1979; Fatty acid and polar lipid composition in the classification of Cellulomonas, Oerskovia and related taxa. J. Appl. Bacteriol. 47:87–95
RaineyF. A, Ward-RaineyN., KroppenstedtR. M., StackebrandtE.1996; The genus Nocardiopsis represents a phylogenetically coherent taxon and a distinct actinomycete lineage: proposal of Nocardiopsaceae fam. nov. Int. J. Syst. Bacteriol. 46:1088–1092
SchumannP., PrauserH., RaineyF. A., StackebrandtE., HirschP.1997; Friedmanniella antárctico gen. nov, sp. nov, an LL-diaminopimelic acid-containing actinomycete from antarctic sandstone. Int. J. Syst. Bacteriol. 47:278–283
StackebrandtE., KochC., GvozdiakO., SchumannP.1995; Taxonomic dissection of the genus Micrococcus’. Kocuria gen. nov, Nesterenkonia gen. nov, Kytococcus gen. nov, and Micrococcus Cohn 1872 gen. emend. Int. J. Syst. Bacteriol. 45:682–692
StackebrandtE., RaineyF. A, Ward-RaineyN. L.1997; Proposal for a new hierarchic classification system, Actinobacteria classis nov. Int. J. Syst. Bacteriol. 47:479–491
SteadD. E., SellwoodJ. E., WilsonJ., VineyI.1992; Evaluation of a commercial microbial identification system based on fatty acid profiles for rapid, accurate identification of plant pathogenic bacteria. J. Appl. Bacteriol. 72:315–321
TamuraT, NakagaitoY., NishiiT., HasegawaT., StackebrandtE., YokotaA.1994; A new genus of the order Actinomycetales, Couchioplanes gen. nov, with descriptions of Couchioplanes caeruleus (Horan and Brodsky 1986) comb. nov. and Couchioplanes caeruleus subsp. azureus subsp. nov. Int. J. Syst. Bacteriol. 44:193–203
UchidaK, AidaK.1984; An improved method for the glycolate test for simple identification for the acyl type of bacteria cell walls. J. Gen. Appl. Microbiol. 30:131–134
YamadaK, KomagataK.1972; Taxonomic studies on coryneform bacteria. IV. Morphological, cultural, biochemical, and physiological characteristics. J. Gen. Appl. Microbiol. 18:399–416
YokotaA, TakeuchiM., SakaneT., WeissN.1993; Proposal of six new species of the genus Aureobacterium and transfer of Flavobacterium esteraromaticum Omelianski to the genus Aureobacterium as Aureobacterium esteraromaticum comb. nov. Int. J. Syst. Bacteriol. 43:555–564
YokotaA, TamuraT., HasegawaT., HuangL. H.Catenuloplanes japonicus gen. nov, sp. nov, nom. rev, a new genus of the order Actinomycetales. Int. J. Syst. Bacteriol. 43:805–812