The genetic structure of isolates recovered from three species (, and ) grown in Mexico was examined. Among 41 isolates, 18 electrophoretic types (ETs) were distinguished by multilocus enzyme electrophoresis of five metabolic enzymes. The mean genetic diversity, 0.64, indicated that there was great genetic diversity in the population sampled. Most isolates (63%) fell into two closely related clusters (clusters I and II) and were the types most frequently isolated from the root nodules of and . ET cluster III isolates were frequent nodule occupants of The isolates also were assigned to three main groups by using Curie point pyrolysis mass spectrometry. In general, the multilocus enzyme electrophoretic data and pyrolysis mass spectrometric data agreed. We determined the 16S rRNA sequences of representative isolates and of USDA 6 and found that the lupine isolates were highly related to the type strain, although not all type strains (subcultures maintained in different bacterial collections) had identical small-subunit rRNA.


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