1887

Abstract

Fourteen strains of a hitherto unknown catalase-positive, aerobic, gram-positive coryneformlike organism were isolated from the milk of sheep with subclinical mastitis from different regions of Spain. The strains phenotypically closely resembled one another and biochemically were similar to and subsp. The results of chemotaxonomic investigations were consistent with membership in the genus , and comparative 16S rRNA gene sequencing studies showed that the unknown bacterium from sheep was indeed a member of the genus Within the genus the new bacterium formed a distinct subline that exhibited >4% sequence divergence with other species. Based on both phenotypic and phylogenetic findings, a new species, , is proposed for the organisms from mastitic sheep. The type strain of is CECT 4843 (= S-8).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-47-4-1082
1997-10-01
2024-06-19
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/47/4/ijs-47-4-1082.html?itemId=/content/journal/ijsem/10.1099/00207713-47-4-1082&mimeType=html&fmt=ahah

References

  1. Bauer A. W., Kirby W. M. M., Sherris J. C., Truck M. 1966; Antibiotic susceptibility testing by standardized single disk method. Am. J. Clin. Pathol. 45:493–496
    [Google Scholar]
  2. Collins M. D., Goodfellow M., Minnikin D. E. 1982; A survey of the structures of mycolic acid in Corynebacterium and related taxa. J. Gen. Microbiol. 128:129–149
    [Google Scholar]
  3. Contreras A., Corrales J. C., Sierra D., Marco J. 1995; Prevalence and aetiology of non-clinical intramammary infection in Murciano-Granadina goats. Small Rum. Res. 17:71–78
    [Google Scholar]
  4. Cruz M., Serrano E., Montoro V., Marco J., Romero M., Baselga R., Albizu I., Moreno B. 1994; Etiology and prevalence of subclinical mastitis in the Manchega sheep at mild-late lactation. Small Rum. Res. 14:175–180
    [Google Scholar]
  5. Devereux J., Haeberli P., Smithies O. 1984; A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res. 12:387–395
    [Google Scholar]
  6. Felsenstein J. 1989; PHYLIP–phylogeny inference package (version 3.2). Cladistics 5:164–166
    [Google Scholar]
  7. Fthenakis G. C. 1994; Prevalence and aetiology of subclinical mastitis in ewes of southern Greece. Small Rum. Res. 13:293–300
    [Google Scholar]
  8. Funke G., von Graevenitz A., Clarridge J. E. III, Bernard K. A. 1997; Clinical microbiology of coryneform bacteria. Clin. Microbiol. Rev. 10:125–159
    [Google Scholar]
  9. Funke G., Bernard K. A., Busher C., Pfyffer G. E., Collins M. D. 1995; Corynebacterium glucuronolyticum sp. nov. isolated from male patients with genitourinary infections. Med. Microbiol. Lett. 4:204–215
    [Google Scholar]
  10. Funke G., Lawson P. A., Collins M. D. 1995; Heterogeneity within Centers for Disease Control and Prevention coryneform group ANF-l-like bacteria and description of Corynebacterium auris sp. nov. Int. J. Syst. Bacteriol. 45:735–739
    [Google Scholar]
  11. García-Rodríguez J. A., Garcia Sanchez J. E., Muñoz Bellido J. L., Nebreda Mayoral T., García Sanchez E., García García I. 1991; In vitro activity of 79 antimicrobial agents against Corynebacterium group D2. Antimicrob. Agents Chemother. 35:2140–2143
    [Google Scholar]
  12. Honkanen-Buzalski T., Myllys V., Kulkas L. 1996; Prevalence of bovine mastitis in Finland. Suom. Elenail. 102:191–197
    [Google Scholar]
  13. Kämpfer P., Kroppenstedt R. M. 1996; Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can. J. Microbiol. 42:989–1005
    [Google Scholar]
  14. Klatte S., Kroppenstedt R. M., Rainey F. A. 1994; Rhodococcus opacues sp. nov., an unusual nutritionally versatile Rhocococcus species. Syst. Appl. Microbiol. 14:355–360
    [Google Scholar]
  15. Lafi S. Q., Al-Rawashdeh O. F., Ereifej K. I., Hailat N. Q. 1994; Incidence of clinical mastitis and prevalence of subclinical udder infections in Jordanian dairy cattle. Prev. Vet. Med. 18:89–98
    [Google Scholar]
  16. Minnikin D. E., Hutchinson I. G., Caldicott A. B., Goodfellow M. 1980; Thin-layer chromatography of methanolysates of mycolic acid-containing bacteria. J. Chromatogr. 188:221–133
    [Google Scholar]
  17. Pascual C., Lawson P. A., Farrow J. A. E., Navarro Giménez M., Collins M. D. 1995; Phylogenetic analysis of the genus Corynebacterium based on 16S rRNA gene sequences. Int. J. Syst. Bacteriol. 45:724–728
    [Google Scholar]
  18. Riegel P., de Briel D., Prévost G., Jehl F., Monteil H., Mink R. 1993; Taxonomie study of Corynebacterium group ANF-1 strains: proposal of Corynebacterium afermentans sp. nov. containing the subspecies C. afermentans subsp. afermentans subsp. nov. and C. afermentans subsp. lipophilum subsp. nov. Int. J. Syst. Bacteriol. 43:287–292
    [Google Scholar]
  19. Riegel P., Ruimy R., de Briel D., Prévost G., Jehl F., Bimet F., Christen R., Monteil H. 1995; Genomic diversity and phylogenetic relationship among lipid-requiring diphtheroids from humans and characterization of Corynebacterium macginleyi sp. nov. Int. J. Syst. Bacteriol. 45:128–133
    [Google Scholar]
  20. Riegel P., Ruimy R., de Briel D., Prévost G., Jehl F., Christen R., Monteil H. 1995; Corynebacterium argentoratense sp. nov. from human throat. Int. J. Syst. Bacteriol. 45:533–537
    [Google Scholar]
  21. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4:406–425
    [Google Scholar]
  22. Schleifer K. H., Kandier O. 1972; Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol. Rev. 36:407–477
    [Google Scholar]
  23. Schreuder B. E. C., Laak E. A., Gee A. L. W., Ter-Laak E. A., DeGee A. L. W. 1990; Corynebacterium pseudotuberculosis in milk of CL affected goats. Vet. Rec. 127:387
    [Google Scholar]
  24. Soto A., Zapardiel J., Soriano F. 1994; Evaluation of API Coryne system for identifying coryneform bacteria. J. Clin. Pathol. 47:756–759
    [Google Scholar]
  25. Stefanakis A., BoScos C., Alexopoulos C., Samartzi F. 1995; Frequency of subclinical mastitis and observations on somatic cell counts in ewes’ milk in northern Greece. Anim. Sci. 61:69–76
    [Google Scholar]
  26. von Graevenitz A., Funke G. 1996; An identification scheme for rapidly and aerobically growing gram-positive rods. Zentralbl. Bakteriol. 284:246–254
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-47-4-1082
Loading
/content/journal/ijsem/10.1099/00207713-47-4-1082
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error