sp. nov., from the Horsefly (Diptera: Tabanidae) Free

Abstract

Spiroplasma strain TALS-2 from the viscera of the striped horsefly, , collected in Georgia was serologically distinct from other species, groups, putative groups, and subgroups. Light and electron microscopy of cells of strain TALS-2 revealed helical motile cells surrounded only by a single cytoplasmic membrane. The organism grew in M1D and SP-4 liquid media. Growth also occurred in 1% serum fraction medium and in conventional horse serum medium. Growth in liquid media was serum dependent. The strain passed through 220-nm filter pores, but was retained in filters with 100-nm pores. The optimum temperature for growth was 30°C. Multiplication occurred at temperatures from 20 to 37°C, with a doubling time at the optimum temperature of 5.6 h in M1D broth. Strain TALS-2 catabolized glucose but hydrolyzed neither arginine nor urea. The guanine-plus-cytosine content of the DNA was 25 ± 1 mol%. The genome size was 1,390 kbp. Six isolates serologically similar to strain TALS-2 were obtained from the same host in coastal Georgia. Three strains closely related to strain TALS-2 were isolated from the horsefly in Costa Rica. Strain TALS-2 (= ATCC 51749), a representative of group XXVII, is designated the type strain of a new species,

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1997-10-01
2024-03-29
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References

  1. Carle P., Saillard C., Bové J. M. 1983 Determination of guanine plus cytosine content of DNA. 301–308 Razin S., Tully J. G.ed Methods in mycoplasmology 1 Academic Press; New York, N.Y.:
    [Google Scholar]
  2. Carle P., Laigret F., Tully J. G., Bové J. M. 1995; Heterogeneity of genome sizes within the genus Spiroplasma. Int. J. Syst. Bacteriol. 45:178–181
    [Google Scholar]
  3. Clark T. B. 1982; Spiroplasmas: diversity of arthropod reservoirs and hostparasite relationships. Science 217:57–59
    [Google Scholar]
  4. Clark T. B., Peterson R. V., Whitcomb R. F., Renegar R. B., Hackett K. J., Tully J. G. 1984; Spiroplasmas in the Tabanidae. Isr. J. Med. Sci. 20:1002–1005
    [Google Scholar]
  5. French F. E., Whitcomb R. F., Hagan D. V., Rafter J. A., Konai M., Clark E. A. 1992; Dynamics of Spiroplasma infections in tabanid (Díptera: Tabanidae) flies, laboratory transmission, and in vitro tests. IOM Lett. 2:114
    [Google Scholar]
  6. French F. E., Whitcomb R. F., Tully J. G., Hackett K. J., Clark E. A., Henegar R. B., Rose D. L. 1990; Tabanid spiroplasmas of the southeast USA: new groups, and correlation with host life history strategy. Zentralbl. Bakteriol. Int. J. Med. Microbiol. 20:919–921
    [Google Scholar]
  7. French F. E., Whitcomb R. F., Tully J. G., Williamsom D. L., Henegar R. B. 1996; Spiroplasmas of Tabanus lineóla. IOM Lett. 4:211–212
    [Google Scholar]
  8. Gasparich G. E., Saillard C., Clark E. A., Konai M., French F. E., Tully J. G., Hackett K. J., Whitcomb R. F. 1993; Serologic and genomic relatedness of group VIII and group XVII spiroplasmas and subdivisions of spiroplasma group VIII. Int. J. Syst. Bacteriol. 43:338–341
    [Google Scholar]
  9. Hackett K. J., Whitcomb R. F., French F. E., Tully J. G., Gasparich G. E., Rose D. L., Carle P., Bové J. M., Henegar R. B., Clark T. B., Konai M., Clark E. A., Williamson D. L. 1996; Spiroplasma corruscae sp. nov., a new species from a firefly beetle (Coleóptera: Lampyridae) and tabanid flies (Díptera: Tabanidae). Int. J. Syst. Bacteriol. 46:947–950
    [Google Scholar]
  10. International Committee on Systematic Bacteriology Subcommittee on the Taxonomy of Mollicutes 1995; Revised minimum standards for descriptions of new species of the class Mollicutes (division Tenericutes). Int. J. Syst. Bacteriol. 45:605–612
    [Google Scholar]
  11. Konai M., Clark E. A., Camp M., Whitcomb R. F. 1996; Temperature ranges and growth optima of Spiroplasma (Mollicutes·. Spiroplasmataceae) species. Curr. Microbiol. 32:314–319
    [Google Scholar]
  12. Konai M., Whitcomb R. F., French F. E., Tully J. G., Rose D. L., Carle P., Bové J. M., Hackett K. J., Henegar R. B., Clark T. B., Williamson D. L. 1997; Spiroplasma litorale sp. nov. from tabanid flies (Tabanidae: Díptera) in the southeastern United States. Int. J. Syst. Bacteriol. 47:359–362
    [Google Scholar]
  13. Le Goff F., Humphery-Smith I., Leclercq M., Chastel C. 1991; Spiro plasmas from European Tabanidae. Med. Vet. Entomol. 5:143–144
    [Google Scholar]
  14. Markham P. G., Clark T. B., Whitcomb R. F. 1983 Culture techniques for spiroplasmas from arthropods. 217–223 Tully J. G., Razin S.ed Methods in mycoplasmology 2 Academic Press; New York, N.Y.:
    [Google Scholar]
  15. Mouchés C., Bové J. M., Tully J. G., Rose D. L., McCoy R. E., Carle P., Junca P., Garnier M., Saillard C. 1983; Spiroplasma apis, a new species from the honey bee Apis mellifera. Ann. Inst. Pasteur Microbiol. 134:383–397
    [Google Scholar]
  16. Moulder R. W., French F. E., Whitcomb R. F., Henegar R. 1996; Spiroplasma carriage in two spring tabanid flies. IOM Lett. 4:200–201
    [Google Scholar]
  17. Rose D. L., Tully J. G., Bové J. M., Whitcomb R. F. 1993; A test for measuring growth responses of mollicutes to serum and polyoxyethylene sorbitan. Int. J. Syst. Bacteriol. 43:527–532
    [Google Scholar]
  18. Tully J. G. 1983 Cloning and filtration techniques for mycoplasmas. 173–177 Razin S., Tully J. G.ed Methods in mycoplasmology 1 Academic Press; New York, N.Y.:
    [Google Scholar]
  19. Tully J. G., Whitcomb R. F. 1991 The genus Spiroplasma. 1960–1980 Balows A., Trüper H. G., Dworkin M., Harder W., Schleifer K.-H.ed The prokaryotes, 2nd. 2 Springer-Verlag; New York, N.Y.:
    [Google Scholar]
  20. Tully J. G., Bové J. M., Laigret F., Whitcomb R. F. 1993; Revised taxonomy of the class Mollicutes·. proposed elevation of a monophyletic cluster of arthropod-associated mollicutes to ordinal rank (Entomoplasmatales ord. nov.), with provision for familial rank to separate species with nonhelical morphology (Entomoplasmataceae fam. nov.) from helical species (Spiroplasmataceae), and emended descriptions of the order Mycoplasmatales, family Mycoplasmataceae. Int. J. Syst. Bacteriol. 43:378–385
    [Google Scholar]
  21. Tully J. G., Rose D. L., Clark E., Carle P., Bové J. M., Henegar R. B., Whitcomb R. F., Colflesh D. E., Williamson D. L. 1987; Revised group classification of the genus Spiroplasma (class Mollicutes), with proposed new groups XII to XXIII. Int. J. Syst. Bacteriol. 37:357–364
    [Google Scholar]
  22. Whitcomb R. F. 1983 Culture media for spiroplasmas. 147–158 Razin S., Tully J. G.ed Methods in mycoplasmology 1 Academic Press; New York, N.Y.:
    [Google Scholar]
  23. Whitcomb R. F., Bové J. M., Chen T. A., Tully J. G., Williamson D. L. 1987; Proposed criteria for an interim serogroup classification for members of the genus Spiroplasma (class Mollicutes). Int. J. Syst. Bacteriol. 37:82–84
    [Google Scholar]
  24. Whitcomb R. F., French F. E., Tully J. G., Carle P., Henegar R. B., Hackett K. J., Adams J., Williamson D. L. Spiroplasma species, groups, and subgroups from North American Tabanidae. Curr. Microbiol. in press
    [Google Scholar]
  25. Whitcomb R. F., French F. E., Tully J. G., Gasparich G., Rose D. L., Carle P., Bove J. M., Henegar R. B., Konai M., Hackett K. J., Adams J., Clark T. B., Williamson D. L. 1997; Spiroplasma chrysopicola sp. nov., Spiroplasma gladiatoris sp. nov., Spiroplasma helicoides sp. nov., and Spiroplasma tabanidicola sp. nov., from tabanid (Diptera: Tabanidae) flies. Int. J. Syst. Bacteriol. 47:713–719
    [Google Scholar]
  26. Whitcomb R. F., French F. E., Tully J. G., Gasparich G. E., Bove J. M., Carle P., Clark E. A., Henegar R. B. 1992; Tabanid spiroplasma serovars. IOM Lett. 2:115
    [Google Scholar]
  27. Whitcomb R. F., French F. E., Tully J. G., Rose D. L., Carle P. M., Bove J. M., Henegar R. B., Konai M., Hackett K. J., Adams J. R., Williamson D. L. 1997; Spiroplasma montanense sp. nov., from Hybomitra horse flies at northern latitudes in North America. Int. J. Syst. Bacteriol. 47:720–723
    [Google Scholar]
  28. Whitcomb R. F., Gasparich G. E., French F. E., Tully J. G., Rose D. L., Carle P., Bove J. M., Henegar R. B., Konai M., Hackett K. J., Adams J. R., Clark T. B., Williamson D. L. 1996; Spiroplasma syrphidicola sp. nov., from a syrphid (Diptera: Syrphidae) fly. Int. J. Syst. Bacteriol. 46:797–801
    [Google Scholar]
  29. Williamson D. L., Tully J. G., Whitcomb R. F. 1979; Serological relationships of spiroplasmas as shown by combined deformation and metabolism inhibition tests. Int. J. Syst. Bacteriol. 29:345–351
    [Google Scholar]
  30. Williamson D. L., Tully J. G., Whitcomb R. F. 1989 The genus Spiroplasma. 71–111 Whitcomb R. F., Tully J. G.ed The mycoplasmas 5 Academic Press; New York, N.Y.:
    [Google Scholar]
  31. Williamson D. L., Whitcomb R. F., Tully J. G. 1979; The spiroplasma deformation test, a new serological method. Curr. Microbiol. 1:203–207
    [Google Scholar]
  32. Williamson D. L., Whitcomb R. F., Tully J. G., Gasparich G. E., Rose D. L., Carle P., Bove J. M., Hackett K. J., Henegar R. B., Konai M., Chastel C., French F. E. Revised group classification of the genus Spiroplasma. Submitted for publication
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