Spiroplasma strain TALS-2 from the viscera of the striped horsefly, , collected in Georgia was serologically distinct from other species, groups, putative groups, and subgroups. Light and electron microscopy of cells of strain TALS-2 revealed helical motile cells surrounded only by a single cytoplasmic membrane. The organism grew in M1D and SP-4 liquid media. Growth also occurred in 1% serum fraction medium and in conventional horse serum medium. Growth in liquid media was serum dependent. The strain passed through 220-nm filter pores, but was retained in filters with 100-nm pores. The optimum temperature for growth was 30°C. Multiplication occurred at temperatures from 20 to 37°C, with a doubling time at the optimum temperature of 5.6 h in M1D broth. Strain TALS-2 catabolized glucose but hydrolyzed neither arginine nor urea. The guanine-plus-cytosine content of the DNA was 25 ± 1 mol%. The genome size was 1,390 kbp. Six isolates serologically similar to strain TALS-2 were obtained from the same host in coastal Georgia. Three strains closely related to strain TALS-2 were isolated from the horsefly in Costa Rica. Strain TALS-2 (= ATCC 51749), a representative of group XXVII, is designated the type strain of a new species,


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