Thirty-three fluorescent strains isolated from tomato pith necrosis (FPTPN strains) and 89 strains were studied by numerical taxonomy. In the dendrogram of distances, the strains constituted a single phenon (phenon 1), whereas 17 of the 33 FPTPN strains clustered in a separate phenon (phenon 2). The other 16 FPTPN strains were included in phena consisting of well-characterized fluorescent species or were isolated phenotypes. Phena 1 and 2 were distinguished by fluorescence on King B medium, accumulation of poly-β;-hydroxybutyrate, production of levan, and assimilation of sorbitol. DNA-DNA hybridization showed that is a true genomic species (66 to 100% DNA relatedness) and that the FPTPN strains of phenon 2 were divided into three genomic groups. Genomic groups 1 and 2 were not distinct from each other phenotypcally, and genomic group 3 could be distinguished from genomic groups 1 and 2 only on the basis of assimilation of citraconate and laevulinate. Genomic groups 1 and 2 are related to (40 to 55% DNA relatedness), whereas genomic group 3 is less closely related to (20 to 23% DNA relatedness). The lipopolysaccharide patterns on electrophoresis gels and fatty acid profiles of strains belonging to genomic groups 1 through 3 are different from each other and from the lipopolysaccharide patterns and fatty acid profiles of . However, cross-reactions were observed between and the FPTPN strain genomic groups, indicating that there are common epitopes of the lipopolysaccharides. The three FPTPN strain genomic groups were not named as species but were designated genomospecies FP1, FP2, and FP3.


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