1887

Abstract

Abstract

Four strains of a previously unknown, catalase-negative, facultatively anaerobic, gram-positive, rod-shaped organism originating from humans were characterized by biochemical, chemical, and molecular taxonomic methods. The four strains phenotypically closely resembled one another, and although they possessed characteristics consistent with membership in the genus , they differed from all previously recognized species of this genus. The results of comparative 16S rRNA gene sequencing studies demonstrated that the unknown human bacterium was phylogenetically a member of the genus Within the genus the unidentified bacterium formed a loose, but statistically significant, association with a subgroup which included , the type species of the genus. 16S rRNA sequence divergence values of >6%, however, unequivocally demonstrated that the unidentified bacterium represents a new subline of the genus A new species, , is proposed for the four new isolates. The type strain of is CCUG 27294.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-47-3-885
1997-07-01
2024-11-13
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/47/3/ijs-47-3-885.html?itemId=/content/journal/ijsem/10.1099/00207713-47-3-885&mimeType=html&fmt=ahah

References

  1. Devereux J., Haeberli P., Smithies O. 1984; A comprehensive of sequence analysis programs for the VAX. Nucleic Acids Res. 12:387–395
    [Google Scholar]
  2. Felsenstein J. 1989; PHYLIP—phylogeny inference package (version 3.2). Cladistics 5:164–166
    [Google Scholar]
  3. Funke G., Martinetti-Lucchini G., Pfyffer G. E., Marchiani M., von Gravenitz A. 1993; Characteristics of CDC group 1 and group 1-like coryneform bacteria isolated from clinical specimens. J. Clin. Microbiol. 31:2907–2912
    [Google Scholar]
  4. Funke G., Stubbs S., Graevenitz A., Collins M. D. 1994; Assignment of human-derived CDC group 1 coryneform bacteria and CDC group 1-like coryneform bacteria to the genus Actinomyces as Actinomyces neuii subsp. neuii sp. nov., subsp. nov., and Actinomyces neuii subsp. anitratus subsp. nov.. Int. J. Syst. Bacteriol. 44:167–171
    [Google Scholar]
  5. Funke G., Ramos C. Pascual, Garayzabal J. F., Weiss N., Collins M. D. 1995; Description of human-derived Centers for Disease Control coryneform group 2 bacteria as Actinomyces bernardiae sp. nov.. Int. J. Syst. Bacteriol. 45:57–60
    [Google Scholar]
  6. Funke G., von Graevenitz A., Clarridge J. E. III, Bernard K. A. 1997; Clinical microbiology of coryneform bacteria. Clin. Microbiol. Rev. 10:125–159
    [Google Scholar]
  7. Johnson J. L., Moore L. V. H., Kaneko B., Moore W. E. C. 1990; Actinomyces georgiae sp. nov., Actinomyces gerencseriae sp. nov., designation of two genospecies of Actinomyces naeslundii, and inclusion of A. naeslundii serotypes II and III and Actinomyces viscosus serotype II in A. naeslundii genospecies 2. Int. J. Syst. Bacteriol. 40:273–286
    [Google Scholar]
  8. Pascual C., Foster G., Collins M. D. 1997; Phylogenetic analysis of the genus Actinomyces based on 16S rRNA gene sequences: description of Arcanobacterium phocae sp. nov., Arcanobacterium bernardiae comb, nov., and Arcanobacterium pyogenes comb. nov.. Int. J. Syst. Bacteriol. 47:46–53
    [Google Scholar]
  9. Pot B., Vandamme P., Kersters K. 1994; Analysis of electrophoretic whole-organism protein fingerprints. 493–521 Goodfellow M., O’Donnell A. G. Modern microbial methods. Chemical methods in prokaryotic systematics J. Wiley and Sons, Ltd.; Chichester, United Kingdom:
    [Google Scholar]
  10. Schaal K. P. 1986; Genus Actinomyces. 1383–1418 Sneath P. H. A., Mair N. S., Sharpe M. E., Holt J. G. Bergey’s manual of systematic bacteriology 2 The Williams & Wilkins Co.; Baltimore, Md.:
    [Google Scholar]
  11. Schaal K. P. 1992; The genera Actinomyces, Arcanobacterium, and Rothia. 850–905 Balows A., Trüper H. G., Dworkin M., Harder W., Schleifer K. H. The prokaryotes I Springer-Verlag; New York, N.Y.:
    [Google Scholar]
  12. Vandamme P., Vancanneyt M., Pot B., Mels L., Hoste B., Dewettinck D., Vlaes L., Van Den Borre C., Higgins R., Hommez J., Kersters K., Butzler J.-P., Goossens H. 1992; Polyphasic taxonomic study of the emended genus Arcobacter with Arcobacter butzleri comb. nov. and Arcobacter skirrowii sp. nov., an aerotolerant bacterium isolated from veterinary sources. Int. J. Syst. Bacteriol. 42:344–356
    [Google Scholar]
  13. Wüst J., Stubbs S., Weiss N., Funke G., Collins M. D. 1995; Assignment of Actinomyces pyogenes-like (CDC coryneform group E) bacteria to the genus Actinomyces as Actinomyces radingae sp. nov. and Actinomyces turicensis sp. nov.. Lett. Appl. Microbiol. 20:76–81
    [Google Scholar]
/content/journal/ijsem/10.1099/00207713-47-3-885
Loading
/content/journal/ijsem/10.1099/00207713-47-3-885
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error