1887

Abstract

The genetic and phylogenetic relationships for strains of and its relatives were compared by an analysis of the results of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of whole-cell proteins, DNA-DNA hybridization, and full 16S rRNA gene sequencing. The strains of . formed a cluster which was distinct from those of other species in the clustering analysis of SDS-PAGE. DNA-DNA relatedness between A-1BS (type strain of . ) and the type or reference strains for , . , . , . , and cluster U, an unnamed rhizobial group, ranged from 4.4 to 43.8%. The phylogenetic analysis based on the 16S rRNA gene sequences showed that . was closely related to the phylogenetic branch and could be distinguished from the other four species in this branch. These results further confirmed that these bacteria constitute a distinct rhizobial species.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-47-3-874
1997-07-01
2024-04-18
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/47/3/ijs-47-3-874.html?itemId=/content/journal/ijsem/10.1099/00207713-47-3-874&mimeType=html&fmt=ahah

References

  1. Brosius J., Palmer M. L., Kennedy P. J., Noller H. F. 1978; Complete nucleotide sequence of a 16S ribosomal RNA from Escherichia coli. Proc. Natl. Acad. SciUSA 75:4801–4805
    [Google Scholar]
  2. Chen W., Wang E., Wang S., Li Y., Chen X., Li Y. 1995; Characteristics of Rhizobium tianshanense sp. nov., a moderately and slowly growing root nodule bacterium isolated from an arid saline environment in Xinjiang, People’s Republic of China. Int. J. Syst. Bacteriol 45:153–159
    [Google Scholar]
  3. Chen W. X., Li G. S., Qi Y. L., Wang E. T., Yuan H. L., Li J. L. 1991; Rhizobium huakuii sp. nov. isolated from the root nodules of Astragalus sinicus. Int. J. Syst. Bacteriol 41:275–280
    [Google Scholar]
  4. Chen W. X., Yan G. H., Li J. L. 1988; Numerical taxonomic study of fast-growing soybean rhizobia and a proposal that Rhizobium fredii be assigned to Sinorhizobium gen. nov. Int. J. Syst. Bacteriol 38:392–397
    [Google Scholar]
  5. Chun J., Goodfellow M. 1995; A phylogenetic analysis of the genus Nocardia with 16S rRNA gene sequences. Int. J. Syst. Bacteriol 45:240–245
    [Google Scholar]
  6. De Lajudie P., Willems A., Pot B., Dewettinck D., Maestrojuan G., Neyra M., Collins M. D., Dreyfus B., Kersters K., Gillis M. 1994; Polyphasic taxonomy of rhizobia: emendation of the genus Sinorhizobium and description of Sinorhizobium meliloti comb. nov., Sinorhizobium saheli sp. nov., and Sinorhizobium teranga sp. nov. Int. J. Syst. Bacteriol 44:715–733
    [Google Scholar]
  7. De Ley J., Cattoir H., Reynaerts A. 1970; The quantitative measurement of DNA hybridization from renaturation rates. Eur. J. Biochem 12:133–142
    [Google Scholar]
  8. Dreyfus B., Garcia J. L., Gillis M. 1988; Characterization of Azorhizobium caulinodans gen. nov., sp. nov., a stem-nodulating nitrogen-fixing bacterium isolated from Sesbania rostrata. Int. J. Syst. Bacteriol 38:89–98
    [Google Scholar]
  9. Eardley B. D., Biever M. A. 1995; Potential chimeras in the 16S rRNA genes of Rhizobium and other genera. Presented at the 15th North American Conference on Symbiotic Nitrogen Fixation, North Carolina State UniversityRaleigh, N.C.13 to 17August 1995
    [Google Scholar]
  10. Felsenstein J. 1993; PHYLIP-(Phylogeny Inference Package), version 3.572c. Department of Genetics, University of Washington; Seattle, Wash:
    [Google Scholar]
  11. Flores M., Gonzalez V., Pardo M. A., Leija A., Martinez E., Romero D., Pinero D., Eavila G. D., Palacios R. 1988; Genomic instability in Rhizobium phaseoli. J. Bacteriol 170:1191–1196
    [Google Scholar]
  12. Gillis M. Personal communication
    [Google Scholar]
  13. Graham P. H., Sadowsky M. J., Keyser H. H., Barnet Y. M., Bradley R. S., Cooper J. E., De Ley J., Jarvis B. D. W., Roslycky E. B., Strijdom B. W., Young J. W. 1991; Proposed minimal standards for the description of new genera and species of root- and stem-nodulating bacteria. Int. J. Syst. Bacteriol 41:582–587
    [Google Scholar]
  14. Haukka K. E., Lindstrom K., Young J. W. 1996; Diversity of partial 16S rRNA sequences among and within strains of African rhizobia isolated from Acacia and Prosopis. Syst. Appl. Microbiol 19:352–359
    [Google Scholar]
  15. Jarvis B. D. W., Van Berkum P., Chen W. X., Nour S. M., Fernandez M. P., Cleyet-Marel J. C., Gillis M. 1997; Transfer of Rhizobium loti Rhizobium huakuii Rhizobium ciceri Rhizobium mediterraneum and Rhizobium tianshanense to Mesorhizobium gen. nov. Int. J. Syst. Bacteriol 47:895–898
    [Google Scholar]
  16. Jarvis B. D. W., Pankhurst C. E., Patel J. J. 1982; Rhizobium loti a new species of legume root nodule bacteria. Int. J. Syst. Bacteriol 32:378–380
    [Google Scholar]
  17. Jordan D. C. 1984; Family III. Rhizobiaceae Conn 1938, 321AL. 234–242 Krieg N. R., Holt J. G. Bergey’s manual of systematic bacteriology 1 Williams and Wilkins Co; Baltimore, Md:
    [Google Scholar]
  18. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London) 227:680–685
    [Google Scholar]
  19. Lindstrom K., van Berkum P., Gillis M., Martinez E., Novikova N., Jarvis B. 1995; Report of the round table on Rhizobium taxonomy. 807–810 Tikhonovich I. A., Provorov N. A., Romanov V. I., Newton W. E. Nitrogen fixation: fundamentals and applications Kluwer Academic Publishers; Dordrecht, The Netherlands:
    [Google Scholar]
  20. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from microorganisms. J. Mol. Biol 3:208–218
    [Google Scholar]
  21. Martinez-Romero E., Caballero-Mellado J. 1996; Rhizobium phytogenies and bacterial genetic diversity. Crit. Rev. Plant. Sci 15:113–140
    [Google Scholar]
  22. Noel K. D., Brill W. J. 1980; Diversity and dynamics of indigenous Rhizobium japonicum. Appl. Environ. Microbiol 40:931–938
    [Google Scholar]
  23. Nour S. M., Cleyet-Marel J.-C., Normand P., Fernandez M. P. 1995; Genomic heterogeneity of strains nodulating chickpeas (Cicer arietinum L.) and description of Rhizobium mediterraneum sp. nov. Int. J. Syst. Bacteriol 45:640–648
    [Google Scholar]
  24. Nour S. M., Fernandez M. P., Normand P., Cleyet-Marel J.-C. 1994; Rhizobium ciceri sp. nov., consisting of strains that nodulate chickpeas (Cicer arietinum L.). Int. J. Syst. Bacteriol 44:511–522
    [Google Scholar]
  25. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular cloning: a laboratory manual, 2nd. Cold Spring Harbor Laboratory Press; Cold Spring Harbor, N.Y:
    [Google Scholar]
  26. Smammons D. W., Adams L. D., Nishizawa E. E. 1981; Ultrasensitive silver based color staining of polypeptides in polyacrylamide gels. Electro-phoresis 2:135
    [Google Scholar]
  27. Sneath P. H. A., Sokal R. R. 1973 Numerical taxonomy: the principle and practices of numerical classification W. H. Freeman & Co; San Francisco, Calif:
    [Google Scholar]
  28. Tiesman J., Rizzino A. 1991; A rapid and reliable method for the purification of high-quality plasmid DNA for double strand sequencing. BioTechniques 10:319–328
    [Google Scholar]
  29. Vincent J. M. 1970; A manual for the practical study of root-nodule bacteria. 73–97 International Biological Program handbook 15 Blackwell Scientific Publications, Ltd; Oxford, United Kingdom:
    [Google Scholar]
  30. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E., Stackebrandt E., Starr M. P., Truper H. G. 1987; Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int. J. Syst. Bacteriol 37:463–464
    [Google Scholar]
  31. Wieslander L. 1979; A simple method to recover intact high molecular weight RNA and DNA after electrophoretic separation in low melting temperature agarose gels. Anal. Biochem 98:305
    [Google Scholar]
  32. Willems A., Collins M. D. 1993; Phylogenetic analysis of rhizobia and agrobacteria based on 16S rRNA gene sequences. Int. J. Syst. Bacteriol 43:305–313
    [Google Scholar]
  33. Yanagi M., Yamasato K. 1993; Phylogenetic analysis of the family Rhizobiaceae and related bacteria by sequencing of 16S rRNA gene using PCR and DNA sequencer. FEMS Microbiol. Lett 107:115–120
    [Google Scholar]
  34. Young J. W., Haukka K. E. 1996; Diversity and phylogeny of rhizobia. New Phytol 133:87–94
    [Google Scholar]
  35. Young J. W., Downer H. L., Eardly B. D. 1991; Phylogeny of the phototropic Rhizobium strain BTAil by polymerase chain reaction-based sequencing of a 16S rRNA gene segment. J. Bacteriol 173:2271–2277
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-47-3-874
Loading
/content/journal/ijsem/10.1099/00207713-47-3-874
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error