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Volume 47, Issue 3

sp. nov., a New Mollicute with Unusual Morphology and Genome Size from the Dragonfly Free

Abstract

Spiroplasma strain PALS-1 from the gut of the dragonfly was shown to be distinct from other species, groups, and subgroups of the genus as determined by reciprocal serological metabolism inhibition and deformation tests. However, this strain cross-reacted extensively with representatives of other groups when it was used as an antigen. Electron microscopy of cells of strain PALS-1 revealed cells surrounded by a single cytoplasmic membrane. Light microscopy revealed helical cells that exhibited twisting motility rather than rotatory or flexing motility. Variations in the tightness of coiling were transmitted from one end of the helix to the other. The strain was resistant to penicillin, which confirmed that no cell wall was present. The organism grew well in M1D and SP-4 liquid media under either aerobic or anaerobic conditions. Growth also occurred in 1% serum fraction medium and in conventional horse serum medium. The optimum temperature for growth was 30°C, at which the doubling time was 6.4 h. Multiplication occurred at temperatures from 10 to 32°C. Strain PALS-1 catabolized glucose and hydrolyzed arginine but not urea. The guanine-plus-cytosine content of the DNA was 29 ± 1 mol%. The genome size was 780 kbp, the smallest genome size in the genus . Strain PALS-1 (= ATCC 51748) is designated the type strain of a new species, .

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© Society for General Microbiology, 1997
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1997-07-01
2024-04-25
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References

  1. Aluotto B. B., Wittier R. G., Williams C. O., Faber J. E. 1970; Standardized bacteriologic techniques for characterization of Mycoplasma species. Int. J. Syst. Bacteriol. 20:35–58
     [Google Scholar]
  2. Carle P. Unpublished data
  3. Carle P., Saillard C., Bove J. M. 1983; DNA extraction and purification. Methods Mycoplasmol. 1:295–299
     [Google Scholar]
  4. Carle P., Saillard C., Bove J. M. 1983; Determination of guanine plus cytosine content of DNA. Methods Mycoplasmol. 1:301–308
     [Google Scholar]
  5. Carle P., Laigret F., Tully J. G., Bové J. M. 1995; Heterogeneity of genome sizes within the genus Spiroplasma. Int. J. Syst. Bacteriol. 45:178–181
     [Google Scholar]
  6. Clark T. B. 1982; Spiroplasmas: diversity of arthropod reservoirs and hostparasite relationships. Science 212:57–59
     [Google Scholar]
  7. Hackett K. J. 1990; Derrick Edward Award: adaptational biology and spiroplasmas. Zentralbl. Bakteriol. Hyg. Suppl. 20:2–32
     [Google Scholar]
  8. Hackett K. J., Clark T. B. 1989 Ecology of spiroplasmas. 113–200 Whitcomb R. F., Tully J. G.ed The mycoplasmas 5 Academic Press; San Diego, Calif.:
     [Google Scholar]
  9. Hackett K. J., Whitcomb R. F., Henegar R. B., Wagner A. G., Clark E. A., Tully J. G., Green F., McKay W. H., Santini P., Rose D. L., Anderson J. J., Lynn D. E. 1990; Mollicute diversity in arthropod hosts. Zentralbl. Bakteriol. Hyg. Suppl. 20:441–454
     [Google Scholar]
  10. Hackett K. J., Whitcomb R. F., Tully J. G., Lloyd J. E., Anderson J. J., Clark T. B., Henegar R. B., Rose D. L., Clark E. A., Vaughn J. L. 1992; Lampyridae (Coleóptera): a plethora of mollicute associations. Microb. Ecol. 23:181–193
     [Google Scholar]
  11. International Committee on Systematic Bacteriology Subcommittee on the Taxonomy of Mollicutes 1995; Revised minimal standards for descriptions of new species of the class Mollicutes. Int. J. Syst. Bacteriol. 45:605–612
     [Google Scholar]
  12. Junca P., Saillard C., Tully J. G., Garcia-Jurado O., Degorce-Dumas J. R., Mouches C., Vignault J. C., Vogel R., McCoy R., Whitcomb R., Williamson D., Latrille J., Bové J. M. 1980; Caractérisation de spiroplasmes isolés d’insectes et de fleurs de France continentale, de Corse et du Maroc. Proposition pour une classification des spiroplasmes. C. R. Acad. Sci. Ser. D 290:1209–1212
     [Google Scholar]
  13. Konai M., Clark E. A., Whitcomb R. F. 1992; Temperature ranges, growth optima, and growth rates of Spiroplasma (Spiroplasmataceae: class Mollicutes) species. Curr. Microbiol. 32:1–7
     [Google Scholar]
  14. Markham P. G., Clark T. B., Whitcomb R. F. 1983; Culture techniques for spiroplasmas from arthropods. Methods Mycoplasmol. 2:217–223
     [Google Scholar]
  15. Rose D. L., Tully J. G., Bové J. M., Whitcomb R. F. 1993; A test for measuring growth responses of mollicutes to serum and polyoxyethylene sorbitan. Int. J. Syst. Bacteriol. 43:527–532
     [Google Scholar]
  16. Tully J. G. 1983; Cloning and filtration techniques for mycoplasmas. Methods Mycoplasmol. 1:173–177
     [Google Scholar]
  17. Tully J. G. 1995 Determination of cholesterol and polyoxyethylene growth requirements of mollicutes. 381–389 Razin S., Tully J. G.ed Molecular and diagnostic procedures in mycoplasmology 1 Academic Press; San Diego, Calif.:
     [Google Scholar]
  18. Tully J. G., Razin S.ed 1996 Molecular and diagnostic procedures in mycoplasmology, vol. 2. 460–462 Academic Press; San Diego, Calif.:
     [Google Scholar]
  19. Tully J. G., Whitcomb R. F. 1991 The genus Spiroplasma. 1960–1980 Balows A., Trüper H. G., Dworkin M., Harder W., Schleifer K. H.ed The prokaryotes, 2nd. vol. 2. Springer-Verlag; New York, N.Y.:
     [Google Scholar]
  20. Tully J. G., Bové J. M., Laigret F., Whitcomb R. F. 1993; Revised taxonomy of the class Mollicutes: proposed elevation of a monophyletic cluster of arthropod-associated mollicutes to ordinal rank (Entomoplasmatales ord. nov.), with provision for familial rank to separate species with nonhelical morphology (Entomoplasmataceae fam. nov.) from helical species (Spiroplasmataceae), and emended descriptions of the order Mycoplasmatales, family Mycoplasmataceae. Int. J. Syst. Bacteriol. 43:378–385
     [Google Scholar]
  21. Tully J. G., Rose D. L., Clark E., Carle P., Bové J. M., Henegar R. B., Whitcomb R. F., Colflesh D. E., Williamson D. L. 1987; Revised group classification of the genus Spiroplasma (class Mollicutes), with proposed new groups XII to XXIII. Int. J. Syst. Bacteriol. 37:357–364
     [Google Scholar]
  22. Whitcomb R. F. 1983; Culture media for spiroplasmas. Methods Mycoplasmol. 1:147–158
     [Google Scholar]
  23. Whitcomb R. F., Hackett K. J. 1996 Identification of spiroplasmas from insects. 313–322 Tully J. G., Razin S.ed Molecular and diagnostic procedures in mycoplasmology 2 Academic Press; San Diego, Calif.:
     [Google Scholar]
  24. Whitcomb R. F., Tully J. G., Williamson D. L., Bové J. M., French F. E., Konai M., Gasparich G., Abalain-Colloc M., Saillard C., Chastel C., Carle P., Rose D. L., Henegar R., Clark E. A., Hackett K. J. 1992; Revised classification of spiroplasmas. IOM Lett. 2:134
     [Google Scholar]
  25. Williamson D. L. 1983; Specialized electron microscopic techniques for spiroplasmas in plant and insect tissues. Methods Mycoplasmol. 1:71–76
     [Google Scholar]
  26. Williamson D. L., Tully J. G., Whitcomb R. F. 1979; Serological relationships of spiroplasmas as shown by combined deformation and metabolism inhibition tests. Int. J. Syst. Bacteriol. 29:345–351
     [Google Scholar]
  27. Williamson D. L., Tully J. G., Whitcomb R. F. 1989 The genus Spiroplasma. 71–111 Whitcomb R. F., Tully J. G.ed The mycoplasmas 5 Academic Press; San Diego, Calif.:
     [Google Scholar]
  28. Williamson D. L., Whitcomb R. F., Tully J. G. 1978; The spiroplasma deformation test, a new serological method. Curr. Microbiol. 1:203–207
     [Google Scholar]
  29. Williamson D. L., Whitcomb R. F., Tully J. G., Gasparich G., Rose D. L., Carle P., Bové J. M., Hackett K. J., Henegar R. B., Konai M., Chastel C., French F. E. Revised group classification of the genus Spiroplasma. Submitted for publication
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Spiroplasma platyhelix sp. nov., a New Mollicute with Unusual Morphology and Genome Size from the Dragonfly Pachydiplax longipennis
Int J Syst Evol Microbiol 47, 763 (1997); https://doi.org/10.1099/00207713-47-3-763
/content/journal/ijsem/10.1099/00207713-47-3-763
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