1887

Abstract

A mesophilic, acetate-oxidizing, sulfur-reducing bacterium, strain NZ27, was isolated from anoxic mud from a freshwater sulfur spring. The cells were ovoid, motile, and gram negative. In addition to acetate, the strain oxidized pyruvate, succinate, and fumarate. Sulfur flower could be replaced by polysulfide as an electron acceptor. Ferric nitrilotriacetic acid was reduced in the presence of pyruvate; however, this reduction did not sustain growth. These phenotypic characteristics suggested that strain NZ27 is affiliated with the genus . A phylogenetic analysis based on the results of comparative 16S ribosomal DNA sequencing confirmed that strain NZ27 belongs to the cluster in the recently proposed family “” in the delta subgroup of the . In addition, the results of DNA-DNA hybridization studies confirmed that strain NZ27 represents a novel species. , a name tentatively used in previous publications, is the name proposed for strain NZ27 in this paper.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-47-3-754
1997-07-01
2022-05-25
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/47/3/ijs-47-3-754.html?itemId=/content/journal/ijsem/10.1099/00207713-47-3-754&mimeType=html&fmt=ahah

References

  1. Bache R., Pfennig N. Unpublished data
  2. Bonch-Osmolovskaya E. A., Solokova T. G., Kostrikina N. A., Zavarzin G. A. 1990; Desulfurella acetivorans gen. nov. and sp. nov., a new thermophilic sulfur-reducing eubacterium. Arch. Microbiol. 153:151–155
    [Google Scholar]
  3. Cashion P., Holder-Franklin M. A., McCully J., Franklin M. 1977; A rapid method for the base ratio determination of bacterial DNA. Anal. Biochem. 81:461–466
    [Google Scholar]
  4. Cline J. D. 1969; Spectrophotometric determination of hydrogen sulfide in natural waters. Limnol. Oceanogr. 14:454–458
    [Google Scholar]
  5. Coates J. D., Lonegran D. J., Philips E. J. P., Jenter H., Lovley D. 1995; Desulfuromonas palmitatis sp. nov., a marine dissimilatory Fe(III) reducer that can oxidize long-chain fatty acids. Arch. Microbiol. 164:406–413
    [Google Scholar]
  6. De Ley J., Cattoir H., Reynaerts A. 1970; The quantitative measurement of DNA hybridization from renaturation rates. Eur. J. Biochem. 12:133–142
    [Google Scholar]
  7. Emmert D. B., Stoehr P. J., Stoessner G., Cameron G. N. 1994; The European Bioinformatics Institute (EBI databases). Nucleic Acids Res. 22:3445–3449
    [Google Scholar]
  8. Felsenstein J. 1993 PHYLIP (phylogeny inference package), version 3.5c Department of Genetics; University of Washington, Seattle.:
    [Google Scholar]
  9. Finster K., Tanimoto Y., Bak F. 1992; Fermentation of methanethiol and dimethylsulfide by a newly isolated methanogenic bacterium. Arch. Microbiol. 157:425–430
    [Google Scholar]
  10. Finster K., Bak F. 1993; Complete oxidation of propionate, valerate, succinate, and other organic compounds by newly isolated types of marine, anaerobic, mesophilic, gram-negative, sulfur-reducing eubacteria. Appl. Environ. Microbiol. 59:1452–1460
    [Google Scholar]
  11. Finster K., Bak F., Pfennig N. 1994; Desulfuromonas acetexigens sp. nov., a dissimilatory sulfur-reducing eubacterium from anoxic freshwater sediments. Arch. Microbiol. 161:328–332
    [Google Scholar]
  12. Fowler V. J., Widdel F., Pfennig N., Woese C. R., Stackebrandt E. 1986; Phylogenetic relationship of sulfate- and sulfur-reducing eubacteria. Syst. Appl. Microbiol. 8:32–41
    [Google Scholar]
  13. Gerhardt P., Murray R. G. E., Costilow R. N., Nester E. W., Wood W. A., Krieg N. R., Phillips G. B.ed 1981 Manual of methods for general bacteriology American Society for Microbiology; Washington, D.C.:
    [Google Scholar]
  14. Gillis M., De Ley J., De Cleene M. 1970; The determination of molecular weight of bacterial genome DNA from renaturation rates. Eur. J. Biochem. 12:143–153
    [Google Scholar]
  15. Huss V. A. R., Festl H., Schleifer K. H. 1983; Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst. Appl. Microbiol. 4:184–192
    [Google Scholar]
  16. Jahnke K.-D. 1992; BASIC computer program for evaluation of spectroscopic DNA renaturation data from GILFORD SYSTEM 2600 spectrophotometer on PC/XT/AT type personal computer. J. Microbiol. Methods 15:61–73
    [Google Scholar]
  17. Jukes T. H., Cantor C. R. 1969 Evolution of protein molecules. 21–132 Munro H. N.ed Mammalian protein metabolism 3 Academic Press; New York, N.Y.:
    [Google Scholar]
  18. Lane D. J. 1991 16S/23S rRNA sequencing. 115–175 Stackebrandt E., Goodfellow M.ed Nucleic acid techniques in bacterial systematics John Wiley & Sons, Inc.; New York, N.Y.:
    [Google Scholar]
  19. Liesack W., Finster K. 1994; Phylogenetic analysis of five strains of gram-negative, obligate anaerobic, sulfur-reducing bacteria and the description of Desulfuromusa gen. nov., including Desulfuromusa kysingii sp. nov., Desulfuromusa bakii sp. nov., and Desulfuromusa succinoxidans sp. nov. Int. J. Syst. Bacteriol. 44:753–758
    [Google Scholar]
  20. Lonergan D. J., Jenter H. L., Coates J. D., Phillips E. J. P., Schmidt T. M., Lovley D. R. 1996; Phylogenetic analysis of dissimilatory Fe(III)-reducing bacteria. J. Bacteriol. 178:2402–2408
    [Google Scholar]
  21. Lovley D. R., Philipps E. J. P. 1987; Rapid assay for microbially reducible ferric iron in aquatic sediments. Appl. Environ. Microbiol. 53:1536–1540
    [Google Scholar]
  22. Lovley D. R., Giovannoni S. J., White D. C., Champine J. E., Phillips E. J. P., Gorby A., Goodwin S. 1993; Geobacter metallireducens gen. nov. sp. nov., a microorganism capable of coupling the complete oxidation of organic compounds to the reduction of iron and other metals. Arch. Microbiol. 159:336–344
    [Google Scholar]
  23. Maidak B. L., Larsen N., McCaughey M. J., Overbeek R., Olsen G. J., Fogel K., Blandy J., Woese C. R. 1994; The Ribosomal Database Project. Nucleic Acids Res. 22:3485–3487
    [Google Scholar]
  24. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J. Mol. Biol. 5:109–118
    [Google Scholar]
  25. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int. J. Syst. Bacteriol. 39:159–167
    [Google Scholar]
  26. Pfennig N. 1978; Rhodocyclus purpureus gen. nov. and sp. nov., a ringshaped, vitamin B12-requiring member of the family Rhodospirillaceae. Int. J. Syst. Bacteriol. 28:283–288
    [Google Scholar]
  27. Pfennig N., Biebl H. 1976; Desulfuromonas acetoxidans gen. nov. and sp. nov., a new anaerobic, sulfur-reducing, acetate-oxidizing bacterium. Arch. Microbiol. 110:3–12
    [Google Scholar]
  28. Roden E. E., Lovley D. R. 1993; Dissimilatory Fe(III) reduction by the marine microorganism Desulfuromonas acetoxidans. Appl. Environ. Microbiol. 59:734–742
    [Google Scholar]
  29. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int. J. Syst. Bacteriol. 44:846–849
    [Google Scholar]
  30. Tamaoka J., Komagato K. 1984; Determination of DNA base composition by reverse-phase high-performance liquid chromatography. FEMS Microbiol. Lett. 25:125–128
    [Google Scholar]
  31. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E., Stackebrandt E., Starr M. P., Triiper H. G. 1987; Report of the Ad Hoc Committee on Reconciliation of Approaches to Bacterial Systematics. Int. J. Syst. Bacteriol. 37:463–464
    [Google Scholar]
  32. Widdel F. 1988 Microbiology and ecology of sulfate- and sulfur-reducing bacteria. 469–585 Zehnder A. J. B.ed Biology of anaerobic microorganisms John Wiley & Sons, Inc.; New York, N.Y.:
    [Google Scholar]
  33. Widdel F., Bak F. 1992 Gram-negative mesophilic sulfate-reducing bacteria. 3352–3378 Balows A., Triiper H. G., Dworkin M., Harder W., Schleifer K. H.ed The prokaryotes, 2nd. 4 Springer; New York, N.Y.:
    [Google Scholar]
  34. Widdel F., Pfennig N. 1992 The genus Desulfuromonas and other Gram-negative sulfur-reducing eubacteria. 3379–3389 Balows A., Triiper H. G., Dworkin M., Harder W., Schleifer K. H.ed The prokaryotes, 2nd. 4 Springer; New York, N.Y.:
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-47-3-754
Loading
/content/journal/ijsem/10.1099/00207713-47-3-754
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error