1887

Abstract

Organisms with the typical characteristics of mycoplasmas were isolated from joints and lungs of crocodiles. The results of growth inhibition tests and immunobinding assays showed that the 24 mycoplasma strains isolated were identical and distinct from previously described , and species. These organisms represent a new species, for which the name is proposed. ferments glucose and maltose, does not produce films and spots, does not hydrolyze arginine, esculin, and urea, reduces tetrazolium chloride, and possesses phosphatase activity. It lyses and adsorbs bovine, ovine, and rabbit erythrocytes. Cholesterol or serum is required for growth. The optimum growth temperature is 37°C. The G+C content of the DNA is 27.6 mol%. This organism causes exudative polyarthritis in crocodiles. The type strain of is strain MP145 (= ATCC 51981).

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/content/journal/ijsem/10.1099/00207713-47-3-742
1997-07-01
2024-04-13
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References

  1. Aluotto B. B., Wittier R. G., Williams C. O., Faber J. E. 1970; Standardized bacteriologie techniques for the characterization of Mycoplasma species. Int. J. Syst. Bacteriol. 20:35–58
    [Google Scholar]
  2. Bilofsky H. S., Burks C. 1988; The GenBank (R) genetic sequence data bank. Nucleic Acids Res. 16:1861–1864
    [Google Scholar]
  3. Brown D. R., Clippinger T. L., Helmick K. E., Schumacher I. M., Bennett R. A., Johnson C. M., Vliet K. A., Jacobsen E. R., Brown M. B. 1996; Mycoplasma isolation during a fatal epizootic of captive alligators (Alligator mississippiensis) in Florida. IOM Lett. 4:42–43
    [Google Scholar]
  4. Brown D. R., Crenshaw B. C., McLaughlin G. S., Schumacher I. M., McKenna C. E., Klein P. A., Jacobsen E. R., Brown M. B. 1995; Taxonomic analysis of the tortoise mycoplasmas Mycoplasma agassizii and Mycoplasma testudinis by 16S rRNA gene sequence comparison. Int. J. Syst. Bacteriol. 45:348–350
    [Google Scholar]
  5. Clyde W. A. Jr. 1964; Mycoplasma species identification based upon growth inhibition by specific antisera. J. Immunol. 92:958–965
    [Google Scholar]
  6. Cottew G. S. 1983; Recovery and identification of caprine and ovine mycoplasmas. Methods Mycoplasmal. 2:91–104
    [Google Scholar]
  7. Flossdorf J. 1983; A rapid method for the determination of the base composition of bacterial DNA. J. Microbiol. Methods 1:305–311
    [Google Scholar]
  8. Freundt E. A., Andrews B. E., Erno H., Kunze M., Black F. T. 1973; The sensitivity of Mycoplasmatales to sodium-polyanethol sulfonate and digitonin. Zentralbl. Bakteriol. Parasitenkd. Infektionskr. Hyg. Abt. 1 Grig. Reihe A 225:104–112
    [Google Scholar]
  9. Gardella R. S., DelGiudice R. A. 1983; Hemagglutination, hemadsorption, and hemolysis. Methods Mycoplasmol. 1:379–384
    [Google Scholar]
  10. Kirchhoff H., Beyene P., Fischer M., Flossdorf J., Heitmann J., Khattab B., Lopatta D., Rosengarten R., Seidel G., Yousef C. 1987; Mycoplasma mobile sp. nov., a new species from fish. Int. J. Syst. Bacteriol. 37:192–197
    [Google Scholar]
  11. Kotani H., McGarrity G. J. 1985; Rapid and simple identification of mycoplasmas by immunobinding. J. Immunol. Methods 85:257–267
    [Google Scholar]
  12. Mohan K., Foggin C. M., Muravarirwa P., Honywill J., Pawandiwa A. 1995; Mycoplasma-associated polyarthritis in farmed crocodiles (Crocodylus niloticus) in Zimbabwe. Onderstepoort J. Vet. Res. 62:45–49
    [Google Scholar]
  13. Morton H. E., Roberts R. J. 1967; Production of anti-mycoplasma (PPLO) antibodies in rabbits. Proc. Soc. Exp. Biol. Med. 125:538–543
    [Google Scholar]
  14. Razin S. 1983; Urea hydrolysis. Methods Mycoplasmol. 1:351–353
    [Google Scholar]
  15. Robertson J. A., Vekris A., Bebear C., Stemke G. W. 1993; Polymerase chain reaction using 16S rRNA gene sequences distinguishes the two biovars of Ureaplasma urealyticum. J. Clin. Microbiol. 31:824–830
    [Google Scholar]
  16. Rose D. L., Tully J. G. 1983; Detection of beta-glucosidase: hydrolysis of esculin and arbutin. Methods Mycoplasmol. 1:385–389
    [Google Scholar]
  17. Rosengarten R., Behrens A., Stetefeld A., Heller M., Ahrens M., Sachse K., Yogev D., Kirchhoff H. 1994; Antigen heterogeneity among isolates of Mycoplasma bovis is generated by high-frequency variation of diverse membrane surface proteins. Infect. Immun. 62:5066–5074
    [Google Scholar]
  18. Subcommittee on the Taxonomy of Mollicutes of the International Committee on Systematic Bacteriology 1995; Revised minimum standards for description of new species of the class Mollicutes (division Tenericutes). Int. J. Syst. Bacteriol. 45:605–612
    [Google Scholar]
  19. Tully J. G. 1983; Tests for digitonin sensitivity and sterol requirement. Methods Mycoplasmol. 1:355–362
    [Google Scholar]
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