RT Journal Article SR Electronic(1) A1 Picardeau, M. A1 Bull, T. J. A1 Prod’Hom, G. A1 Pozn1Ak, A. L. A1 Shanson, D. C. A1 Vincent, V.YR 1997 T1 Comparison of a New Insertion Element, IS1407, with Established Molecular Markers for the Characterization of Mycobacterium celatum JF International Journal of Systematic and Evolutionary Microbiology, VO 47 IS 3 SP 640 OP 644 DO https://doi.org/10.1099/00207713-47-3-640 PB Microbiology Society, SN 1466-5034, AB Abstract Genomic analyses of 18 Mycobacterium celatum strains obtained from different patients in three countries (United States, United Kingdom, and France) were performed; the methods used in this study were restriction fragment length polymorphism (RFLP) analysis, pulsed-field gel electrophoresis (PFGE) analysis, and PCR restriction analysis (PRA) of the hsp-65 gene. A new insertion sequence, IS1407 (GenBank accession no. X97307), belonging to the IS256 family, was identified in M. celatum type 1 strains and was characterized by sequencing. When a probe for Mycobacterium xenopi IS1395-like sequences was used, the RFLP analysis of M. celatum type 1 strains revealed that they contained three or four copies of IS1407 in identical genomic positions, while this element was absent in all M. celatum type 2 strains. PFGE performed with three different endonucleases revealed a unique large restriction fragment (LRF) pattern for M. celatum type 1 strains, whereas the LRF patterns obtained for M. celatum type 2 strains were polymorphic. Moreover, PFGE of nondigested genomic DNA revealed extrachromosomal elements in M. celatum type 2. The type strain of M. celatum type 3 could not be differentiated from M. celatum type 1 strains on the basis of the results of the RFLP analysis, the PFGE analysis, and the PRA of 1S1407. In this study we confirmed that M. celatum types 1 and 2 represent distinct genomic clusters and that the molecular markers in M. celatum type 2 exhibit greater polymorphism than the molecular markers in M. celatum type 1., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/00207713-47-3-640