1887

Abstract

Phenotypic studies, as well as the reaction of genomic DNA with a 16S ribosomal DNA (sequence of variable regions V1 to V4)-based -specific PCR system and oligonucleotide probe, the presence of sequences homologous to in both and , and the results of DNA-DNA hybridization experiments performed with the and type strains and one additional strain, showed that these two species form a homogeneous group. In addition, evidence was found for the presence of genes in , and was shown to fix atmospheric nitrogen. Therefore, the names and should be considered synonyms. As was capable of fixing nitrogen and fixation without inhibition by nitrate is a major characteristic of the group, we propose that be included in the species

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-47-2-569
1997-01-01
2022-08-16
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/47/2/ijs-47-2-569.html?itemId=/content/journal/ijsem/10.1099/00207713-47-2-569&mimeType=html&fmt=ahah

References

  1. Ash C., Priest F. G., Collins M. D. 1993; Molecular identification of rRNA group 3 bacilli (Ash, Farrow, Wallbanks and Collins) using a PCR probe test. Proposal for the creation of a new genus Paenibacillus. Antonie van Leeuwenhoek 64:253–260
    [Google Scholar]
  2. Cannon F. C., Riedel G. E., Ausubel F. M. 1979; Overlapping sequences of Klebsiella pneumoniae nif DNA cloned and characterized. Mol. Gen. Genet 174:59–66
    [Google Scholar]
  3. Collins M. D., Lawson P. A., Willems A., Cordoba J. J., Fernandez-Garayzabal J., Garcia P., Cai J., Hippe H., Farrow J. A. E. 1994; The phylogeny of the genus Clostridium·, proposal of five new genera and eleven new species combinations. Int. J. Syst. Bacteriol 44:812–826
    [Google Scholar]
  4. Gordon R. E., Haynes W. C., Pang H. N. 1973 The genus Bacillus. Agriculture Handbook no. 427. Agricultural Research Service, U.S. Department of Agriculture; Washington, D.C:
    [Google Scholar]
  5. Rosado A. S., Seldin L., Wolters A. C., van Elsas J. D. 1996; Quantitative 16S rDNA targeted polymerase chain reaction and oligonucleotide hybridization for the detection of Paenibacillus azotofixans in soil and the wheat rhizosphere. FEMS Microbiol. Ecol 19:153–164
    [Google Scholar]
  6. Seldin L., van Elsas J. D., Penido E. G. C. 1984; Bacillus azotofixans sp. nov., a nitrogen-fixing species from Brazilian soils and grass roots. Int. J. Syst. Bacteriol 34:451–456
    [Google Scholar]
  7. Seldin L., Dubnau D. 1985; Deoxyribonucleic acid homology among Bacillus polymyxa, Bacillus macerans, Bacillus azotofixans, and other nitrogen-fixing Bacillus strains. Int. J. Syst. Bacteriol 35:151–154
    [Google Scholar]
  8. Seldin L., Penido E. G. C. 1986; Identification of Bacillus azotofixans using API tests. Antonie van Leeuwenhoek 52:403–409
    [Google Scholar]
  9. Seldin L., Bastos M. C. F., Penido E. G. C. 1989 Identification of Bacillus azotofixans nitrogen fixation genes using heterologous nif probes. 179–187 Skinner F. A., Boddey R. M., Fendrik I.ed Nitrogen fixation with non-legumes Kluwer Academic Publishers; Dordrecht, The Netherlands:
    [Google Scholar]
  10. Seldin L. 1992; Primary characterization of the bacteriophage BA-4 from a nitrogen-fixing Bacillus azotofixans strain. Microbios 71:167–177
    [Google Scholar]
  11. Smith L. D. S., Cato E. P. 1974; Clostridium durum, sp. nov., the predominant organism in a sediment core from the Black Sea. Can. J. Microbiol 20:1393–1397
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-47-2-569
Loading
/content/journal/ijsem/10.1099/00207713-47-2-569
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error