1887

Abstract

The complete 5S and 16S ribosomal DNA (rDNA) sequences of the facultatively chemolithotrophic bacterium and results of a comparison of these sequences with homologous sequences from several proteobacterial species supported affiliation of with the β subgroup of the , and form a phylogenetic cluster that comprises some of the thiobacilli capable of mixotrophic growth. This cluster is related to some pseudomonads and species belonging to the β subclass. In addition, a low-frequency restriction fragment analysis (LFRFA) of some mixotrophic thiobacilli and some related species was carried out by using pulsed-field gel electrophoresis (PFGE) to determine the I and I macrorestriction patterns and genome sizes of these organisms. The correlation of the LFRFA results and the 16S rDNA analysis results and the usefulness of the two analyses are discussed. The PFGE fingerprints suggested that sp. strain ATCC 27793 is related to rather than to , as described previously. The distinctive characteristics of the mixotrophic species analyzed in this work, their phylogenetic relatedness, and their physiological differences from other groups belonging to the , including other thiobacilli, suggest that these organisms should be transferred to a new genus, the genus gen. nov.

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1997-01-01
2022-08-18
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