1887

Abstract

Members of the marigold genus of flowering plants (the genus ), which synthesize and accumulate thiophene compounds in their roots, were investigated as potential sources of bacteria able to degrade substituted thiophenes. Batch and continuous enrichment cultures inoculated with compost from root balls of and reproducibly produced the same predominant type of bacterium when they were supplied with thiophene-2-carboxylate (T2C) or thiophene-2-acetate (T2A) as a carbon and energy substrate. This organism was a yellow-pigmented, neutrophilic, mesophilic, gram-negative, pleomorphic, rodshaped bacterium, which we classify as a new species of the genus ; strain TagT2C (= DSM 11105) is the type strain. Strain TagT2C (T = type strain) grew on simple thiophenes, such as T2C, thiophene-3-carboxylate, and T2A, on analogs of these compounds (pyrrole-2-carboxylate and furan-2-carboxylate), and on the condensed thiophene dibenzothiophene. was facultatively autotrophic, fixing carbon dioxide by means of ribulose bisphosphate carboxylase, and was able to grow on hydrogen, thiosulfate, or sulfide as an energy substrate. It also grew on a wide range of other heterotrophic, chemolithotrophic, and methylotrophic substrates. Its growth on T2C was optimal at 28 to 31°C and pH 7.6 to 7.8, and the maximum growth rate in batch culture was 0.22 h. The DNA base composition of is 68 mol% G+C. A 16S ribosomal DNA sequence analysis of strain TagT2C showed that this organism represents a distinct lineage within the cluster of the alpha-2 subclass of the . Discrimination of from the other genera in this group and from other species is discussed.

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1997-01-01
2022-09-25
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