1887

Abstract

Spiroplasma strain TN-1 (T = type strain), a strain serologically distinct from other spiroplasma species, groups, and subgroups, was isolated from the gut of a horsefly () from a barrier island off the coast of North Carolina. Related strains were isolated from other spp., , and , from coastal Georgia. Cells of strain TN-1 in culture were helical and motile with an average of 5 to 10 helical turns per cell. Electron microscopic studies determined that the cells of strain TN-1 were surrounded by a single cytoplasmic membrane, and there was no evidence of a cell wall. The spiroplasma grew well in MID and SP-4 liquid media. Serum fraction (1%) medium and conventional horse serum medium also supported growth of strain TN-1. Fried-egg colonies were not produced; instead, the strain produced small diffuse colonies that were surrounded by satellite growth. The optimum temperature for growth was 32°C, but multiplication was observed at temperatures from 10 to 41°C. The doubling time at the optimum temperature (32°C) was 1.6 h. No growth was observed at 5 or 43°C. Spiroplasma strain TN-1 passed through 220-nm filter pores but failed to pass through 100-nm filter pores. Strain TN-1 catabolized glucose but hydrolyzed neither arginine nor urea. The guanine-plus-cytosine content of the DNA was about 25 ± 1 mol%, and the genome size was 1,370 kbp. Based on results from this study and previously published data, strain TN-1 (= ATCC 43211) (group XVIII) is designated the type strain of a new spiroplasma species, .

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1997-01-01
2024-04-13
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