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Volume 47, Issue 2

Comparison of 16S Ribosomal DNA Sequences of All Species Free

Abstract

The phylogenetic relationships of all validly described species of the genus and the type strain of were analyzed by sequencing and comparing 16S ribosomal DNAs (rDNAs). The two genera exhibited a mean sequence similarity value of 96.6%, corresponding to differences at 50 nucleotide positions on average. The species of the genus exhibited relatively high levels of overall sequence similarity; the mean similarity value was 98.2%, which corresponds to an average of 14 mutual nucleotide differences. Within the genus , a group containing , and clustered apart from the main core, whereas formed a third phylogenetic lineage. Due to the very restricted variability in 16S rDNA sequences within the genus , rDNA signatures that have possible diagnostic value for differentiating the species could not be determined with certainty. When sequence similarities were compared with DNA-DNA pairing data determined previously, there was only a limited correlation. This illustrates the different resolving powers of the techniques for determining phylogenetic hierarchies and for species delineation.

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Copyright © 1997 International Union of Microbiological Societies
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1997-01-01
2024-04-18
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Comparison of 16S Ribosomal DNA Sequences of All Xanthomonas Species
Int J Syst Evol Microbiol 47, 328 (1997); https://doi.org/10.1099/00207713-47-2-328
/content/journal/ijsem/10.1099/00207713-47-2-328
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