1887

Abstract

Abstract

We determined the taxonomic status of six species (,, ,, and ) by using the results of 16S rRNA gene sequence and cellular fatty acid composition analyses. Phylogenetic analysis clustered these species closely with the species. Like the species, the six species contained anteiso-C fatty acid as a major cellular fatty acid. The use of a specific PCR primer designed for differentiating the genus from other members of the showed that the six species had the same amplified 16S rRNA gene fragment as members of the genus Based on these observations and other taxonomic characteristics, the six species were transferred to the genus In addition, we propose emendation of the genus .

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1997-01-01
2024-10-09
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References

  1. Alexander B., Priest F. G. 1989; Bacillus glucanolyticus, a new species that degrades a variety of β-glucans. Int. J. Syst. Bacteriol. 39:112–115
    [Google Scholar]
  2. Ash C., Priest F. G., Collins M. D. 1993; Molecular identification of rRNA group 3 bacilli (Ash, Farrow, Wallbanks, and Collins) using a PCR probe test. Proposal for the creation of a new genus Paenibacillus. Antonie van Leeuwenhoek 64:253–260
    [Google Scholar]
  3. Ash C., Farrow J. A. E., Wallbanks S., Collins M. D. 1991; Phylogenetic heterogeneity of the genus Bacillus revealed by comparative analysis of small-subunit-ribosomal RNA sequence. Lett. Appl. Microbiol. 13:202–206
    [Google Scholar]
  4. Cato E. P., George W. L., Finegold S. M. 1986; Genus Clostridium Prazmowski 1880. 1141–1200 Sneath P. H. A., Mair N. S., Sharpe M. E., Holt J. G. Bergey’s manual of systematic bacteriology 2 The Williams and Wilkins Co.; Baltimore, Md.:
    [Google Scholar]
  5. Claus D., Berkeley R. C. W. 1986; Genus Bacillus Cohn 1872. 1105–1140 Sneath P. H. A., Mair N. S., Sharpe M. E., Holt J. G. Bergey’s manual of systematic bacteriology 2 The Williams and Wilkins Co.; Baltimore, Md.:
    [Google Scholar]
  6. Collins M. D., Lawson P. A., Willems A., Cordoba J. J., Fernandez-Garayzabal J., Garcia P., Cai J., Hippe H., Farrow J. A. E. 1994; The phylogeny of the genus Clostridium: proposal of five new genera and eleven new species combinations. Int. J. Syst. Bacteriol. 44:812–826
    [Google Scholar]
  7. Farrow J. A. E., Wallbanks S., Collins M. D. 1992; Phylogenetic analysis of the genera Planococcus, Marinococcus, and Sporosarcina and their relationship to members of the genus Bacillus. FEMS Microbiol. Lett. 93:167–172
    [Google Scholar]
  8. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791
    [Google Scholar]
  9. Fox G. E., Wisotzkey J., Jurtshuk P. Jr. 1992; How close is close: 16S rRNA sequence identity may not be sufficient to guarantee species identity. Int. J. Syst. Bacteriol. 42:166–170
    [Google Scholar]
  10. Gordon R. E., Hynes W. C., Pang C. H. N. 1973 The genus Bacillus. Agricultural Handbook no. 427 U.S. Department of Agriculture; Washington, D.C.:
    [Google Scholar]
  11. Heyndrickx M., Vandemeulebroecke K., Hoste B., Janssen P., Kersters K., De Vos P., Logan N. A., Ali N., Berkeley R. C. W. 1996; Reclassification of Paenibacillus (formerly Bacillus) pulvifaciens (Nakamura 1984) Ash et al. 1994, a later subjective synonym of Paenibacillus (formerly Bacillus) larvae (White 1966) Ash et al. 1994, as a subspecies of P. larvae, with emended description of P. larvae as P. larvae subsp. larvae and P. larvae subsp. pulvifaciens. Int. J. Syst. Bacteriol. 46:270–279
    [Google Scholar]
  12. Heyndrickx M., Vandemeulebroecke K., Scheldeman P., Hoste B., Kersters K., De Vos P., Logan N. A., Aziz A. M., Ali N., Berkeley R. C. W. 1995; Paenibacillus (formerly Bacillus) gordonae (Pichinoty et al. 1986) Ash et al. 1994 is a later subjective synonym of Paenibacillus (formerly Bacillus) validus (Nakamura 1984) Ash et al. 1994: emended description of P. validus. Int. J. Syst. Bacteriol. 45:661–668
    [Google Scholar]
  13. Heyndrickx M., Vandemeulebroecke K., Scheldeman P., Kersters K., De Vos P., Logan N. A., Aziz A. M., Ali N., Berkeley R. C. W. 1996; A polyphasic reassessment of the genus Paenibacillus, reclassification of Bacillus lautus (Nakamura 1984) as Paenibacillus lautus comb. nov. and Bacillus peoriae (Montefusco et al. 1993) as Paenibacillus peoriae comb, nov., and emended description of P. lautus and P. peoriae. Int. J. Syst. Bacteriol. 46:988–1003
    [Google Scholar]
  14. Kanzawa Y., Harada A., Takeuchi M., Yokota A., Harada T. 1995; Bacillus curdlanolyticus sp. nov. and Bacillus kobensis sp. nov., which hydrolyze resistant curdlan. Int. J. Syst. Bacteriol. 45:515–521
    [Google Scholar]
  15. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J. Mol. Evol. 16:111–120
    [Google Scholar]
  16. Komagata K., Suzuki K. 1987; Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol. 19:161–207
    [Google Scholar]
  17. Montefusco A., Nakamura L. K., Labeda D. P. 1993; Bacillus peoriae sp. nov. Int. J. Syst. Bacteriol. 43:388–390
    [Google Scholar]
  18. Nakamura L. K. 1984; Bacillus amylolyticus sp. nov., nom. rev., Bacillus lautus sp. nov., nom. rev., Bacillus pabuli sp. nov., nom. rev., and Bacillus validus sp. nov., nom. rev. Int. J. Syst. Bacteriol. 34:224–226
    [Google Scholar]
  19. Nakamura L. K. 1987; Bacillus alginolyticus sp. nov. and Bacillus chondroitinus sp. nov., two alginate-degrading species. Int. J. Syst. Bacteriol. 37:284–286
    [Google Scholar]
  20. Nakamura L. K. 1987; Bacillus polymyxa (Prazmowski) Mace 1889 deoxyribonucleic acid relatedness and base composition. Int. J. Syst. Bacteriol. 37:391–397
    [Google Scholar]
  21. Nakamura L. K. 1990; Bacillus thiaminolyticus sp. nov., nom. rev. Int. J. Syst. Bacteriol. 40:242–246
    [Google Scholar]
  22. Nakamura L. K. 1996; Paenibacillus apiarius sp. nov., nom. rev. Int. J. Syst. Bacteriol. 46:688–693
    [Google Scholar]
  23. Nielsen P., Rainey F. A., Outtrup F. A., Priest F. G., Fritze D. 1994; Comparative 16S rDNA sequence analysis of some alkaliphilic bacilli and the establishment of a sixth rRNA group within the genus Bacillus. FEMS Microbiol. Lett. 117:61–66
    [Google Scholar]
  24. Niimura Y., Koh E., Yanagida F., Suzuki K., Komagata K., Kozaki M. 1990; Amphibacillus xylanus gen. nov., sp. nov., a facultatively anaerobic sporeforming xylan-digesting bacterium which lacks cytochrome, quinone, and catalase. Int. J. Syst. Bacteriol. 40:297–301
    [Google Scholar]
  25. Priest F. G., Goodfellow M., Todd C. 1988; A numerical classification of the genus Bacillus. J. Gen. Microbiol. 134:1847–1882
    [Google Scholar]
  26. Rainey F. A., Fritze D., Stackebrandt E. 1994; The phylogenetic diversity of thermophilic members of the genus Bacillus as revealed by 16S rDNA analysis. FEMS Microbiol. Lett. 115:205–212
    [Google Scholar]
  27. Russler D., Ludwig W., Schleifer K. H., Lin C., McGill T. J., Wisotzkey J. D., Jurtshuk P. Jr., Fox G. E. 1991; Phylogenetic diversity in the genus Bacillus as seen by 16S rRNA sequencing studies. Syst. Appl. Microbiol. 14:266–269
    [Google Scholar]
  28. Saito N., Nei M. 1987; A neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4:406–425
    [Google Scholar]
  29. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74:5463–5467
    [Google Scholar]
  30. Seldin L., Van Elsas J. D., Penido E. G. L. 1984; Bacillus azotofixans sp. nov., a nitrogen-fixing species from Brazilian soil and grass roots. Int. J. Syst. Bacteriol. 34:451–456
    [Google Scholar]
  31. Shida O., Takagi H., Kadowaki K., Komagata K. 1996; Proposal for two new genera, Brevibacillus gen. nov. and Aneurinibacillus gen. nov. Int. J. Syst. Bacteriol. 46:939–946
    [Google Scholar]
  32. Spanka R., Fritze D. 1993; Bacillus cohnii sp. nov., a new, obligately alkaliphilic, oval-spore-forming Bacillus species with ornithine and aspartic acid instead of diaminopimelic acid in the cell wall. Int. J. Syst. Bacteriol. 43:150–156
    [Google Scholar]
  33. Spring S., Ludwig W., Marquez M. C., Ventosa A., Schleifer K.-H. 1996; Halobacillus gen. nov., with description of Halobacillus litoralis sp. nov. and Halobacillus truperi sp. nov., and transfer of Sporosarcina halophilia to Halobacillus halophilus comb. nov. Int. J. Syst. Bacteriol. 46:492–496
    [Google Scholar]
  34. Suzuki T., Yamasato K. 1994; Phylogeny of spore-forming lactic acid bacteria based on 16S rRNA gene sequences. FEMS Microbiol. Lett. 115:13–18
    [Google Scholar]
  35. Thompson J. D., Higgins D. G., Gibson T. J. 1994; CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 22:4673–4680
    [Google Scholar]
  36. Wisotzkey J. D., Jurtshuk P. Jr., Fox G. E., Deinhard G., Poralla K. 1992; Comparative sequence analysis on the 16S rRNA (rDNA) of Bacillus acidocaldarius, Bacillus acidoterrestris, and Bacillus cycloheptanicus and proposal for creation of a new genus, Alicyclobacillus gen. nov. Int. J. Syst. Bacteriol. 42:263–269
    [Google Scholar]
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