1887

Abstract

Abstract

A new rapidly growing, scotochromogenic mycobacterium was isolated from urine. This strain is thermophilic (it grows at 65°C), tolerates 5% NaCl, and was unable to utilize any of the sugars tested or citrate or to take up iron. The isolate splits benzamide, urea, nicotinamide, and pyrazinamide and is sensitive to streptomycin, ethambutol, cycloserine, ciprofloxacin, and clarithromycin but resistant to isoniazid, rifampin, and prothionamide. These characteristics clearly place this organism in a new mycobacterial species, which was confirmed by the unique 16S rRNA nucleotide sequence. The high level of similarity between this rapid grower and is surprising. For this new rapidly growing scotochromogenic and thermophilic mycobacterium we propose the name sp. nov.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-47-1-86
1997-01-01
2024-06-25
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/47/1/ijs-47-1-86.html?itemId=/content/journal/ijsem/10.1099/00207713-47-1-86&mimeType=html&fmt=ahah

References

  1. Applied Biosystems GmbH. 1994 PRISM Ready Reaction DyeDeoxy terminator cycle sequencing kit protocol handbook Applied Biosystems GmbH; Weiterstadt, Germany:
    [Google Scholar]
  2. Böddinghaus B., Rogall T., Flohr T., Blöcker H., Böttger E. C. 1990; Detection and identification of mycobacteria by amplification of rRNA. J. Clin. Microbiol. 28:1751–1759
    [Google Scholar]
  3. Corpe R. F., Runyon E. H., Lester W. 1963; Status of disease due to unclassified mycobacteria. A statement of the subcommittee of therapy. Am. Rev. Respir. Dis. 87:459–461
    [Google Scholar]
  4. Deutsche Sammlung von Mikroorganismen und Zellkulturen. 1993 Catalogue of strains Deutsche Sammlung von Mikroorganismen und Zellkulturen; Braunschweig, Germany:
    [Google Scholar]
  5. Deutsches Zentralkomitee zur Bekämpfung der Tuberkulose. 1991; Die Bakteriologie der Tuberkulose. Pneumologie 45:753–774
    [Google Scholar]
  6. DIN. 1991 DIN 58943, parts 3, 8, and 9 Beuth Verlag; Berlin, Germany:
    [Google Scholar]
  7. du Moulin G. C., Stottmeier K. D. 1986; Waterborne mycobacteria: an increasing threat to health. ASM News 52:525–529
    [Google Scholar]
  8. Flourny D. J. 1993; Non acid-fastness of Mycobacterium fortuitum. Med. Sci. Res. 21:665
    [Google Scholar]
  9. Goodfellow M., Wayne L. G. 1982; Taxonomy and nomenclature. 472–521 Ratlege C., Stanford J. L. The biology of the mycobacteria Academic Press; London, United Kingdom:
    [Google Scholar]
  10. Graybill J. R., Silva J., Fraser D. W., Lordon R., Rogers E. 1974; Disseminated mycobacteriosis due to Mycobacterium abscessus in two recipients of renal homografts. Am. Rev. Respir. Dis. 109:4–10
    [Google Scholar]
  11. Haas W. H., Kirschner P., Ziesing S., Bremer H.-J., Böttger E. C. 1993; Cervical lymphadenitis in a child caused by a previously unknown mycobacterium. J. Infect. Dis. 167:237–240
    [Google Scholar]
  12. Kent P. T., Kubica G. P. 1985; Public health mycobacteriology: a guide for the level III laboratory. 71–146 U.S. Department of Health and Human Services publication (CDC) 71–146 Centers for Disease Control; Atlanta, Ga.:
    [Google Scholar]
  13. Kirschner P., Meier A., Böttger E. C. 1993; Genotypic identification and detection of mycobacteria—facing novel and uncultured pathogens. 173–190 Pershing D. H., Smith T. F., Tenover F. C., White T. J. Diagnostic molecular microbiology: principles and applications American Society for Microbiology; Washington, D.C.:
    [Google Scholar]
  14. Kroppenstedt R. M. 1985; Fatty acid and menaquinone analysis of actinomycetes and related organisms. Soc. Appl. Bacteriol. Tech. Ser. 20:173–199
    [Google Scholar]
  15. Kroppenstedt R. M., Korn-Wendisch F., Fowler V. J., Stackebrandt E. 1981; Biochemical and molecular genetic evidence for transfer of Actinoplanes armeniacus into the family Streptomycetaceae. Zentralbl. Bakteriol. Parasitenkd. Infektionskr. Hyg. Abt. 1 Orig. Reihe C 2:254–262
    [Google Scholar]
  16. Kubica G. P., Wayne L. G. 198438–164 The mycobacteria—a sourcebook, part A Marcel Dekker, Inc.; New York, N.Y.:
    [Google Scholar]
  17. Kuykendall L. D., Roy M. A., O’Neill J. J., Devine T. E. 1988; Fatty acids, antibiotic resistance, and deoxyribonucleic acid homology groups of Bradyrhizobium japonicum. Int. J. Syst. Bacteriol. 38:358–361
    [Google Scholar]
  18. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int. J. Syst. Bacteriol. 39:159–167
    [Google Scholar]
  19. Miller L. T. 1982; A single derivatization method for bacterial fatty acid methyl esters including hydroxy acids. J. Clin. Microbiol. 16:584–586
    [Google Scholar]
  20. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. 1984; An integrated procedure for the extraction of isoprenoid quinones and polar lipids. J. Microbiol. Methods 2:233–241
    [Google Scholar]
  21. Minnikin D. E., Hutchinson I. G., Caldicott A. B., Goodfellow M. 1980; Thin-layer chromatography of methanolysates of mycolic acid-containing bacteria. J. Chromatogr. 188:221–233
    [Google Scholar]
  22. Naumann L., Rüsch-Gerdes S. Personal communication
    [Google Scholar]
  23. Reischl U., Pulz M., Ehret W., Wolf H. 1994; PCR-based detection of mycobacteria in sputum samples using a simple and reliable DNA extraction protocol. BioTechniques 17:844–846
    [Google Scholar]
  24. Rogall T., Wolters J., Flohr T., Böttger E. C. 1990; Towards a phylogeny and definition of species at the molecular level within the genus Mycobacterium. Int. J. Syst. Bacteriol. 40:323–330
    [Google Scholar]
  25. Runyon E. H., Karlson A. G., Kubica G. P., Wayne L. G. 1980; Mycobacterium. 150–179 Lennette E. H., Balows A., Hausier W. J. Jr., Truant J. P. Manual of clinical microbiology American Society for Microbiology; Washington, D.C.:
    [Google Scholar]
  26. Sanger R., Coulson A. R., Hong G. F., Hill D. F., Petersen G. B. 1982; Nucleotide sequence of bacteriophage lamda DNA. J. Mol. Biol. 162:729–773
    [Google Scholar]
  27. Shinnick T. M., Good R. C. 1994; Mycobacterial taxonomy. Eur. J. Clin. Microbiol. Infect. Dis. 13:889–895
    [Google Scholar]
  28. Springer B., Kirschner P., Rost-Meyer G., Schröder K. H., Kroppenstedt R. M., Böttger E. C. 1993; Mycobacterium interiectum, a new species isolated from a patient with chronic lymphadenitis. Int. J. Syst. Bacteriol. 31:3083–3089
    [Google Scholar]
  29. Springer B., Stockman L., Teschner K., Roberts G. D., Böttger E. C. 1996; Two-laboratory collaborative study on identification of mycobacteria: molecular versus phenotypic methods. J. Clin. Microbiol. 34:296–303
    [Google Scholar]
  30. Stanek J. L., Roberts G. D. 1974; Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl. Microbiol. 28:226–231
    [Google Scholar]
  31. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reverse-phase high-performance liquid chromatography. FEMS Microbiol. Lett. 25:125–128
    [Google Scholar]
  32. Visuvanathan S., Moss M. T., Stanford J. L., Hermon-Taylor J., McFadden J. J. 1989; Simple enzymatic method for isolation of DNA from diverse bacteria. J. Microbiol. Methods 10:59–64
    [Google Scholar]
  33. Wolinsky E. 1979; Non tuberculous mycobacteria and associated diseases. Am. Rev. Respir. Dis. 119:107–159
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-47-1-86
Loading
/content/journal/ijsem/10.1099/00207713-47-1-86
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error