1887

Abstract

Abstract

Six strains isolated from the intestines of juvenile turbot in a fish hatchery in the north of Spain were found to be phenotypically members of the genus However, the phenotypic traits of these organisms did not place them in any of the currently known species. These isolates formed an homogeneous group in which the DNA-DNA similarity values (the differences between the thermal denaturation midpoints of the homologous and heterologous duplexes) with reference strain A089 (T = type strain) ranged from 0 to 1.7°C. The results of a 16S rRNA sequence analysis of A089 placed this strain in the genus in the gamma subclass of the The closest relative is , with a sequence similarity of 97.8%. This group of strains can be easily differentiated from the other species by their clear phenotype. We propose the name sp. nov. for these strains; the type strain is strain A089 (= CECT 4638).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-47-1-58
1997-01-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/47/1/ijs-47-1-58.html?itemId=/content/journal/ijsem/10.1099/00207713-47-1-58&mimeType=html&fmt=ahah

References

  1. Aisina M., Blanch A. R. 1994; A set of keys for biochemical identification of environmental Vibrio species. J. Appl. Bacteriol. 76:79–85
    [Google Scholar]
  2. Alsina M., Blanch A. R. 1994; Improvement and update of a set of keys for biochemical identification of Vibrio species. J. Appl. Bacteriol. 77:719–721
    [Google Scholar]
  3. Alsina M., Martínez-Picado J., 3 Jofre J., Blanch A. R. 1994; A medium for presumptive identification of Vibrio anguillarum. Appl. Environ. Microbiol. 60:1681–1683
    [Google Scholar]
  4. Austin B., Alsina M., Austin D. A., Blanch A. R., Grimont F., Grimont P. A. D., Jofre J., Koblavi S., Larsen J. L., Pedersen K., Tiainen T., Verdonck L., Swings J. 1995; Identification and typing of Vibrio anguillarum: a comparison of different methods. Syst. Appl. Microbiol. 18:285–302
    [Google Scholar]
  5. Baumann P., Baumann L., Bang S. S., Woolkalis M. J. 1980; Reevaluation of the taxonomy of Vibrio, Beneckea and Photobacterium: abolition of the genus Beneckea. Curr. Microbiol. 4:127–132
    [Google Scholar]
  6. Baumann P., Furniss A. L., Lee J. V. 1984; Genus Vibrio. 518–538 Krieg N. R., Holt J. G. Bergey’s manual of systematic bacteriology 1 The Williams & Wilkins Co.; Baltimore, Md.:
    [Google Scholar]
  7. Berg O. 1995; Bacteria associated with early life stages of halibut, Hippoglossus hippoglossus L., inhibit growth of a pathogenic Vibrio sp. J. Fish Dis. 18:31–40
    [Google Scholar]
  8. Blanch A. R., Simón M., Jofre J., Minkoff G. 1991; Bacteria associated with hatchery cultivated turbot: are they implicated in rearing success?. 392–394 Larvi’91—Fish & Crustacean Larviculture Symposium. European Aquaculture Society publication 15 European Aquaculture Society; Ghent, Belgium.:
    [Google Scholar]
  9. Bolinches J., Romalde J. L., Toranzo A. E. 1988; Evaluation of selective media for isolation and enumeration of vibrios from estuarine waters. J. Microbiol. Methods 8:151–160
    [Google Scholar]
  10. Dorsch M., Lane D., Stackebrandt E. 1992; Towards a phylogeny of the genus Vibrio based on 16S rRNA sequences. Int. J. Syst. Bacteriol. 42:58–63
    [Google Scholar]
  11. Farmer J. J., Hickman-Brenner F. W. 1992; The genera Vibrio and Photobacterium. 2952–3011 Balows A., Trüper H. G., Dworkin M., Harder W., Schleifer K.-H. The prokaryotes. A handbook on the biology of bacteria: ecophysiology, isolation, identification, applications, 2nd ed.. Springer-Verlag; New York, N.Y.:
    [Google Scholar]
  12. Felsenstein J. 1982; Numerical methods for inferring phylogenetic trees. Q. Rev. Biol. 57:379–404
    [Google Scholar]
  13. Furniss A. L., Lee J. V., Donovan T. J. 1978 The vibrios. Public Health Laboratory Service Monograph Series no. 11 Public Health Laboratory Service; London, United Kingdom:
    [Google Scholar]
  14. Kita-Tsukamoto K., Oyaizu H., Nanba K., Simidu U. 1993; Phylogenetic relationships of marine bacteria, mainly members of the family Vibrionaceae, determined on the basis of 16S rRNA sequences. Int. J. Syst. Bacteriol. 43:8–19
    [Google Scholar]
  15. Kobayashi T., Enomoto S., Sakazaki R., Kuwahara S. 1963; A new selective medium for pathogenic vibrios: TCBS agar (modified Nakanishi’s agar). Nippon Saikingaku Zasshi 18:387–391
    [Google Scholar]
  16. MacDonell M. T., Colwell R. R. 1985; Phylogeny of the Vibrionaceae, and recommendation for two new genera, Listonella and Shewanella. Syst. App. Microbiol. 6:171–182
    [Google Scholar]
  17. Maidak B. L., Larsen N., MacCaughey M. J., Overbeek R., Olsen G., Fogel K., Blandy J., Woese C. R. 1994; The Ribosomal Database Project. Nucleic Acids Res. 22:3485–3487
    [Google Scholar]
  18. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from microorganisms. J. Mol. Biol. 3:208–218
    [Google Scholar]
  19. Molitoris E., Marii M. A., Joseph S. W., Krichevsky M. I., Fanning G. R., Last G., El-Mishad A. M., Batawi Y. A. El, Colwell R. R. 1989; Numerical taxonomy and deoxyribonucleic acid relatedness of environmental and clinical Vibrio species isolated in Indonesia. Int. J. Syst. Bacteriol. 39:442–449
    [Google Scholar]
  20. Munro P. D., Barbour A., Birkbeck T. H. 1995; Comparison of the growth and survival of larval turbot in the absence of culturable bacteria with those in the presence of Vibrio anguillarum, Vibrio alginolyticus, or a marine Aeromonas sp. Appl. Environ. Microbiol. 61:4425–4428
    [Google Scholar]
  21. Muroga K., Higahsi M., Keitoku J. 1987; The isolation of intestinal microflora of farmed red seabream (Pagrus major) and black seabream (Acanthopagrus schlegeli) at larval and juvenile stages. Aquaculture 65:79–88
    [Google Scholar]
  22. Onarheim A. M., Raa J. 1990; Characteristics and possible biological significance of an autochtonous flora in the intestinal mucosa of seawater fish. 197–201 Lesel R. Microbiology in poecilotherms Elsevier Science Publishers; Amsterdam, The Netherlands:
    [Google Scholar]
  23. Onarheim A. M., Wiik R., Burghardt J., Stackebrandt E. 1994; Characterization and identification of two Vibrio species indigenous to the intestine of fish in cold sea water; description of Vibrio iliopiscarius sp. nov. Syst. Appl. Microbiol. 17:370–379
    [Google Scholar]
  24. Pujalte M. R., Ortigosa M., Urdaci M. C., Garay E., Grimont P. A. D. 1993; Vibrio mytili sp. nov., from mussels. Int. J. Syst. Bacteriol. 43:358–362
    [Google Scholar]
  25. Pujalte M. R., Ortiz-Conde B. A., Steven S. E., Esteve C., Garay E., Colwell R. R. 1992; Numerical taxonomy and nucleic acid studies of Vibrio mediterranei. Syst. Appl. Microbiol. 15:82–91
    [Google Scholar]
  26. Rosselló R., Garcia-Valdés E., Lalucat J., Ursing J. 1991; Genotypic and phenotypic diversity of Pseudomonas stutzeri. Syst. Appl. Microbiol. 14:150–157
    [Google Scholar]
  27. Ruimy R., Breittmayer V., Elbaze P., Lafay B., Boussemart O., Gauthier M., Christen R. 1994; Phylogenetic analysis and assessment of the genera Vibrio, Photobacterium, Aeromonas and Plesiomonas deduced from small-subunit rRNA sequences. Int. J. Syst. Bacteriol. 44:416–426
    [Google Scholar]
  28. Selin Y. M., Harich B., Johnson J. L. 1983; Preparation of labeled nucleic acids (nick translation and iodination) for DNA homology and rRNA hybridization experiments. Curr. Microbiol. 8:127–132
    [Google Scholar]
  29. Smibert R. M., Krieg N. R. 1994; Phenotypic characterization. 607–654 Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Methods for general and molecular bacteriology ASM Press; Washington, D.C.:
    [Google Scholar]
  30. Springer N., Ludwig W., Amann R., Schmidt H. J., Gorth H. D., Schleifer K. H. 1993; Occurrence of fragmented 16S rRNA in an obligate bacterial endosymbiont of Paramecium caudatum. Proc. Natl. Acad. Sci. USA 90:9892–9895
    [Google Scholar]
  31. Strunk O., Ludwig W. Unpublished data
    [Google Scholar]
  32. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol. Lett. 25:125–128
    [Google Scholar]
  33. Tison D. L., Seidler R. J. 1983; Vibrio aestuarianus: a new species from estuarine waters and shellfish. Int. J. Syst. Bacteriol. 33:699–702
    [Google Scholar]
  34. Tjernberg L., Lindh E., Ursing J. 1989; A quantitative bacterial dot method for DNA-DNA hybridization and its correlation to the hydroxyapatite method. Curr. Microbiol. 18:77–81
    [Google Scholar]
  35. Urdaci M. C., Marchand M., Ageron E., Arcos J. M., Sesma B., Grimont P. A. D. 1991; Vibrio navarrensis sp. nov., a species from sewage. Int. J. Syst. Bacteriol. 41:290–294
    [Google Scholar]
  36. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandier O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E., Stackebrandt E., Starr M. P., Triiper H. G. 1987; Report of the Ad Hoc Committee on Reconciliation of Approaches to Bacterial Systematics. Int. J. Syst. Bacteriol. 37:463–464
    [Google Scholar]
  37. Westerdahl J., Olsson C., Conway P. L., Kjelleberg S. 1994; Characterization of turbot (Scophthalmus maximus) associated bacteria with inhibitory effects against the fish pathogen Vibrio anguillarum. Acta Microbiol. Immunol. Hung. 41:403–409
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-47-1-58
Loading
/content/journal/ijsem/10.1099/00207713-47-1-58
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error