1887

Abstract

A systematic phylogenetic analysis of the genus was performed. The 16S rRNA gene sequences of 13 species, an unnamed strain (ATCC 49338), and an -like isolate from sea mammals were determined. Comparative sequence analysis with closely related taxa revealed phylogenetic diversity and internal structure within the genus . In addition, some members of other genera (viz., the genera , and ) were shown to be phylogenetically intermixed with the species. It was evident from both distance and tree topology considerations that the genus is in urgent need of taxonomic revision and requires subdivision into several genera. Based on the results of the present study it is proposed that and be assigned to the genus as comb. nov. and comb. nov., respectively. In addition, a new species, , is proposed for -like bacteria isolated from seals.

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1997-01-01
2024-04-25
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References

  1. Ausubel F. M., Brent R., Kingston R. E., Moore D. D., Seidman J. G., Smith J. A., Struhl K. 1989 Current protocols in molecular biology John Wiley and Sons; Chichester, United Kingdom:
    [Google Scholar]
  2. Collins M. D., Stubbs S., Hommez J., Devriese L. A. 1993; Molecular taxonomic studies of Actinomyces-like bacteria isolated from purulent lesions in pigs and description of Actinomyces hyovaginalis sp. nov. Int. J. Syst. Bacteriol. 43:471–473
    [Google Scholar]
  3. Collins M. D., Jones D., Kroppenstedt R. M., Schleifer K. H. 1982; Chemical studies as a guide to the classification of Corynebacterium pyogenes and ‘Corynebacterium haemolyticum’. J. Gen. Microbiol. 128:335–341
    [Google Scholar]
  4. Collins M. D., Jones D., Schofield G. M. 1982; Reclassification of ‘Corynebacterium haemolyticum’ (MacLean, Liebow & Rosenberg) in the genus Arcanobacterium gen. nov. as Arcanobacterium haemolyticum nom. rev., comb. nov. J. Gen. Microbiol. 128:1279–1281
    [Google Scholar]
  5. Coykendall A. L., Munzenmaier A. J. 1979; Deoxyribonucleic acid hybridization among strains of Actinomyces viscosus and Actinomyces naeslundii. Int. J. Syst. Bacteriol. 29:234–240
    [Google Scholar]
  6. Devereux J., Haeberli P., Smithies D. 1984; A comprehensive set of sequence analysis programmes for the VAX. Nucleic Acids Res. 12:387–395
    [Google Scholar]
  7. Felsenstein J. 1989; PHYLIP—phylogeny inference package (version 3.2). Cladistics 5:164–166
    [Google Scholar]
  8. Fitch W. M., Margoliash E. 1967; Construction of phylogenetic trees: a method based on mutation distances as estimated from cytochrome c sequences is of general applicability. Science 155:279–284
    [Google Scholar]
  9. Funke G., Stubbs S., Graevenitz A., Collins M. D. 1994; Assignment of human-derived CDC group 1 coryneform bacteria and CDC group 1-like coryneform bacteria to the genus Actinomyces as Actinomyces neuii subsp. neuii sp. nov., subsp. nov., and Actinomyces neuii subsp. anitratus subsp. nov. Int. J. Syst. Bacteriol. 44:167–171
    [Google Scholar]
  10. Funke G., Ramos C. Pascual, Garayzabal J. F., Weiss N., Collins M. D. 1995; Description of human-derived Centers for Disease Control coryneform group 2 bacteria as Actinomyces bemardiae sp. nov. Int. J. Syst. Bacteriol. 45:57–60
    [Google Scholar]
  11. Garvie E. I. 1978; Streptococcus rafflnolactis (Orla-Jensen and Hensen), a group N Streptococcus found in raw milk. Int. J. Syst. Bacteriol. 28:190–193
    [Google Scholar]
  12. Gerencser M. A., Slack J. M. 1969; Identification of human strains of Actinomyces viscosus. Appl. Microbiol. 18:80–87
    [Google Scholar]
  13. Gerencser M. A., Slack J. M. 1976; Serological identification of Actinomyces using fluorescent antibody techniques. J. Dent. Res. 55:Special IssueA184–A191
    [Google Scholar]
  14. Hutson R. A., Thompson D. E., Collins M. D. 1993; Genetic interrelationships of saccharolytic Clostridium botulinum types B, E, and F and related clostridia as revealed by small-subunit rRNA gene sequences. FEMS Microbiol. Lett. 108:103–110
    [Google Scholar]
  15. Johnson J. L., Moore L. V. H., Kaneko B., Moore W. E. C. 1990; Actinomyces georgiae sp. nov., Actinomyces gerencseriae sp. nov., designation of two genospecies of Actinomyces naeslundii, and inclusion of A. naeslundii serotypes II and III and Actinomyces viscosus serotype II in A naeslundii genospecies 2. Int. J. Syst. Bacteriol. 40:273–286
    [Google Scholar]
  16. Ludwig W., Kirchhof G., Weizenegger M., Weiss N. 1992; Phylogenetic evidence for the transfer of Eubacterium suis to the genus Actinomyces as Actinomyces suis comb. nov. Int. J. Syst. Bacteriol. 42:161–165
    [Google Scholar]
  17. Reddy C. A., Cornell C. P., Fraga A. M. 1982; Transfer of Corynebacterium pyogenes (Glage) Eberson to the genus Actinomyces as Actinomyces pyogenes (Glage) comb. nov. Int. J. Syst. Bacteriol. 32:419–429
    [Google Scholar]
  18. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4:406–425
    [Google Scholar]
  19. Schaal K. P. 1986; Genus Actinomyces. 1383–1418 Sneath P. H. A., Mair N. S., Sharpe M. E., Holt J. G. Bergey’s manual of systematic bacteriology 2 The Williams & Wilkins Co.; Baltimore, Md.:
    [Google Scholar]
  20. Schaal K. P. 1992; The genera Actinomyces, Arcanobacterium, and Rothia. 850–905 Balows A., Trüper H. G., Dworkin M., Harder W., Schleifer K. H. The prokaryotes I Springer-Verlag; New York, N.Y.:
    [Google Scholar]
  21. Schofield G. M., Schaal K. P. 1981; A numerical taxonomy study of members of the Actinomycetaceae and related taxa. J. Gen. Microbiol. 127:237–259
    [Google Scholar]
  22. Stackebrandt E., Charfreitag O. 1990; Partial 16S rRNA primary structure of five Actinomyces species: phylogenetic implications and development of an Actinomyces israelii-specific oligonucleotide probe. J. Gen. Microbiol. 136:37–43
    [Google Scholar]
  23. Stubbs S., Collins M. D. Unpublished data
    [Google Scholar]
  24. Wüst J., Stubbs S., Weiss N., Funke G., Collins M. D. 1995; Assignment of Actinomyces pyogenes-like (CDC coryneform group E) bacteria to the genus Actinomyces as Actinomyces radingae sp. nov. and Actinomyces turicensis sp. nov. Lett. Appl. Microbiol. 20:76–81
    [Google Scholar]
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