A 5.0-kb region containing the gene was cloned from and sequenced. In addition to the gene, this sequence region also contained the complete sequences for the and genes and partial sequences for the and genes. The order of the above gene sequences in the cloned fragment was found to be . which is similar to the order seen in various other gram-positive groups of bacteria. The Hsp70 homologs from two mycoplasma species, and . contain a number of sequence signatures, including the absence of a large insert in the N-terminal quadrant, that are characteristics of the homologs from gram-positive bacteria and archae-bacteria. A detailed phylogenetic analysis based on Hsp70 sequences was also performed. In neighbor-joining and parsimony trees based on Hsp70 sequences, both mycoplasma species branched with the low-G+C-content gram-positive group of bacteria (e.g., and species) in 87% and 96% of the bootstrap replicates, respectively, indicating their close evolutionary relationship to this group. The phylogenetic trees based on Hsp70 sequences show a polyphyletic branching of archaebacteria with the gram-positive species, which is statistically strongly favored.


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