Restriction Fragment Length Polymorphism Analysis of PCR-Amplified 16S Ribosomal DNA for Rapid Identification of Strains Free

Abstract

Abstract

Twenty-one strains of , , , , and were examined to evaluate the discriminatory value of 16S ribosomal DNA (rDNA) fingerprints. The 16S rDNAs were amplified by PCR by using oligonucleotide primers complementary to 16S rRNA genes. A restriction fragment length polymorphism (RFLP) analysis of the 16S rDNAs was performed with I and I. The four validly described species could be differentiated on the basis of their characteristic 16S rDNA restriction patterns. The strains of gave a restriction pattern identical to that of K161 (T = type strain). This result was anticipated from the previous report that K161 and the strains of “5. have identical 16S rRNA sequences. We found that purification of amplified 16S rDNA products following PCR was necessary for our RFLP analysis.

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1997-01-01
2024-03-29
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References

  1. Barrowcliff D. E., Arblaster P. G. 1968; Farmer’s lung: a study of an early acute fatal case. Thorax 23:490–500
    [Google Scholar]
  2. Frothingham R., Allen R. L., Wilson K. H. 1991; Rapid 16S ribosomal DNA sequencing from a single colony without DNA extraction or purification. BioTechniques 11:42–44
    [Google Scholar]
  3. Greene J. G., Treuhaft M. W., Arnsell R. M. 1981; Hypersensitivity pneumonitis due to Saccharomonospora viridis diagnosed by inhalation challenge. Ann. Allergy 47:449–452
    [Google Scholar]
  4. Greiner-Mai E., Korn-Wendisch F., Kutzner H. J. 1988; Taxonomic revision of the genus Saccharomonospora and description of Saccharomonospora glauca sp. nov.. Int. J. Syst. Bacteriol. 38:398–405
    [Google Scholar]
  5. Greiner-Mai E., Kroppenstedt R. M., Korn-Wendisch F., Kutzner H. J. 1987; Morphological and biochemical characterization and emended descriptions of thermophilic actinomycetes species. Syst. Appl. Microbiol. 9:97–109
    [Google Scholar]
  6. Hiraishi A., Shin Y. K., Ueda Y., Sugiyama J. 1994; Automated sequencing of PCR-amplified 16S rDNA on ‘Hydrolink’ gels. J. Microbiol. Methods 19:145–154
    [Google Scholar]
  7. Kalakoutskii L. V. 1964; A new species of the genus Micropolyspora— Micropolyspora caesia n. sp.. Mikrobiologiya 33:765–768
    [Google Scholar]
  8. Kim S.-B. 1995 Polyphasic taxonomic studies on the genus Saccharomonospora and related taxa University of Newcastle upon Tyne, Newcastle upon Tyne; United Kingdom: Ph.D. thesis
    [Google Scholar]
  9. Kim S.-B., Yoon J.-H., Kim H., Lee S. T., Park Y.-H., Goodfellow M. 1995; A phylogenetic analysis of the genus Saccharomonospora conducted with 16S rRNA gene sequences. Int. J. Syst. Bacteriol. 45:351–356
    [Google Scholar]
  10. Kurup V. P. 1981; Taxonomic study of some members of Micropolyspora and Saccharomonospora. Microbiologica (Bologna) 4:249–259
    [Google Scholar]
  11. Kurup V. P. 1984; Thermophilic actinomycetes, their role in hypersensitivity pneumonitis. 145–159 Ortiz-Ortiz L., Bojalil L. F., Yakoleff V. Biological, biochemical and biomedical aspects of actinomycetes Academic Press; Orlando, Fla.:
    [Google Scholar]
  12. Lechevalier M. P., Lechevalier H. A. 1970; A critical evaluation of the genera of aerobic actinomycetes. 393–405 Prauser H. The Actinomycetales Gustav Fischer Verlag; Jena, Germany:
    [Google Scholar]
  13. Nonomura H., Ohara Y. 1971; Distribution of actinomycetes in soil. X. New genus and species of monosporic actinomycetes. J. Ferment. Technol. 49:895–903
    [Google Scholar]
  14. Runmao H. 1987; Saccharomonospora azurea sp. nov., a new species from soil. Int. J. Syst. Bacteriol. 37:60–61
    [Google Scholar]
  15. Runmao H., Lin C., Guizhen W. 1988; Saccharomonospora cyanea sp. nov.. Int. J. Syst. Bacteriol. 38:444–446
    [Google Scholar]
  16. Sambrook J., Fritsch E. F., Maniatis T. 198914–19 Molecular cloning: a laboratory manual, 2nd ed.. Cold Spring Harbor Laboratory; Cold Spring Harbor, N. Y.:
    [Google Scholar]
  17. Skerman V. B. D., McGowan V., Sneath P. H. A. 1980; Approved lists of bacterial names. Int. J. Syst. Bacteriol. 30:225–420
    [Google Scholar]
  18. Stackebrandt E., Liesack W. 1993; Nucleic acids and classification. 152–189 Goodfellow M., O’Donnell A. G. Handbook of new bacterial systematics Academic Press; London, United Kingdom:
    [Google Scholar]
  19. Weisburg W. G., Barns S. M., Pelletier D. A., Lane D. J. 1991; 16S ribosomal DNA amplification for phylogenetic study. J. Bacteriol. 173:697–703
    [Google Scholar]
  20. Woese C. R. 1987; Bacterial evolution. Microbiol. Rev. 51:221–271
    [Google Scholar]
  21. Yoon J.-H., Kim H., Kim S.-B., Kim H.-J., Kim W. Y., Lee S. T., Goodfellow M., Park Y.-H. 1996; Identification of Saccharomonospora strains with genomic DNA fragments and rRNA gene probes. Int. J. Syst. Bacteriol. 46:502–505
    [Google Scholar]
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