Twenty-one strains of , and were examined to evaluate the discriminatory value of 16S ribosomal DNA (rDNA) fingerprints. The 16S rDNAs were amplified by PCR by using oligonucleotide primers complementary to 16S rRNA genes. A restriction fragment length polymorphism (RFLP) analysis of the 16S rDNAs was performed with I and I. The four validly described species could be differentiated on the basis of their characteristic 16S rDNA restriction patterns. The strains of gave a restriction pattern identical to that of K161 (T = type strain). This result was anticipated from the previous report that K161 and the strains of have identical 16S rRNA sequences. We found that purification of amplified 16S rDNA products following PCR was necessary for our RFLP analysis.


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