1887

Abstract

Abstract

forms part of the resident microbiota in both oral and extraoral sites in humans and animals. It is also involved in infections in such sites. Despite the genetic heterogeneity within the species, it has been divided into five subspecies, the validities of which have been questioned. In the present study, 44 isolates were examined at 21 enzyme loci by using the allozyme electrophoretic technique to establish an accurate genetic framework for taxonomic purposes. Three distinct genetic clusters were identified; one cluster consisted exclusively of extraoral isolates, another cluster consisted predominantly of human oral isolates, and the third cluster consisted of a single human oral isolate. Our results highlight the urgent need for extensive biochemical, immunological, and epidemiological studies to accurately define the systematics of the genus based on the framework derived in this study by using 21 independent genetic characteristics.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-47-1-103
1997-01-01
2022-05-21
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/47/1/ijs-47-1-103.html?itemId=/content/journal/ijsem/10.1099/00207713-47-1-103&mimeType=html&fmt=ahah

References

  1. Bennett K. W., Eley A. 1993; Fusobacteria: new taxonomy and related diseases. J. Med. Microbiol. 39:246–254
    [Google Scholar]
  2. Bolstad A., Skaug N., Jensen H. B. 1991; Use of synthetic oligonucleotide DNA probes for the identification of different strains of Fusobacterium nucleatum. J. Periodontal Res. 26:519–526
    [Google Scholar]
  3. Bolstad A. 1993 Personal communication (University of Bergen)
    [Google Scholar]
  4. Dzink J. L., Sheenan M. T., Socransky S. S. 1990; Proposal of three subspecies of Fusobacterium nucleatum Knorr 1922: Fusobacterium nucleatum subsp. nucleatum subsp. nov., comb, nov.; Fusobacterium nucleatum subsp. polymorphum subsp. nov., nom. rev., comb, nov.; and Fusobacterium nucleatum subsp. vincentii subsp. nov., nom. rev., comb. nov.. Int. J. Syst. Bacteriol. 40:74–78
    [Google Scholar]
  5. Gharbia S. E., Shah H. N. 1992; Fusobacterium nucleatum subsp. fusiforme subsp. nov. and Fusobacterium nucleatum subsp. animalis subsp. nov. as additional subspecies within Fusobacterium nucleatum. Int. J. Syst. Bacteriol. 42:296–298
    [Google Scholar]
  6. Gharbia S. E., Shah H. N. 1990; Heterogeneity within Fusobacterium nucleatum, proposal of four subspecies. Lett. Appl. Microbiol. 10:105–108
    [Google Scholar]
  7. Gharbia S. E., Shah H. N. 1990; Identification of Fusobacterium species by the electrophoretic migration of glutamate dehydrogenase and 2-oxoglutarate reductase in relation to their DNA base composition and peptidoglycan dibasic amino acids. J. Med. Microbiol. 33:183–188
    [Google Scholar]
  8. Gharbia S. E., Shah H. N. 1991; Isolation, purification and characterisation of 2-oxoglutarate reductase from Fusobacterium nucleatum. FEMS Microbiol. Lett. 80:283–288
    [Google Scholar]
  9. Gharbia S. E., Shah H. N., Lawson P. A., Haapsalo M. 1990; The distribution and frequency of Fusobacterium nucleatum subspecies in the human oral cavity. Oral Microbiol. Immunol. 5:324–327
    [Google Scholar]
  10. Hofstad T. 1992; The genus Fusobacterium. 4114–4126 Balows A., Trüper H. G., Dworkin M., Harder W., Schleifer K.-H. The prokaryotes. A handbook on the biology of bacteria: ecophysiology, isolation, identification, applications, 2nd ed.. IV Springer-Verlag; New York, N.Y.:
    [Google Scholar]
  11. Holst E., Goffeng A. R., Andersch B. 1994; Bacterial vaginosis and vaginal microorganisms in idiopathic premature labor and association with pregnancy outcome. J. Clin. Microbiol. 32:176–186
    [Google Scholar]
  12. Lawson P. A., Gharbia S. E., Shah H. N., Clark D. R. 1989; Recognition of Fusobacterium nucleatum subgroups Fn-1, Fn-2 and Fn-3 by ribosomal RNA gene restriction patterns. FEMS Microbiol. Lett. 65:41–46
    [Google Scholar]
  13. Lawson P. A., Gharbia S. E., Shah H. N., Clark D. R., Collins M. D. 1991; Intrageneric relationships of members of the genus Fusobacterium as determined by reverse transcriptase sequencing of small-subunit rRNA. Int. J. Syst. Bacteriol. 41:347–354
    [Google Scholar]
  14. Magee J. T., Hindmarch J. M., Bennett K. W., Duerden B. I., Aries R. E. 1989; A pyrolysis mass spectrometry study of fusobacteria. J. Med. Microbiol. 28:227–236
    [Google Scholar]
  15. Moore W. E. C., Holdeman L. V., Kelley R. W. 1984; Genus II. Fusobacterium Knorr 1922. 631–637 Krieg N. R., Holt J. G. Bergey’s manual of systematic bacteriology 1 Williams and Wilkins; Baltimore, Md.:
    [Google Scholar]
  16. Moore W. E. C., Moore L. V. H. 1994; The bacteria of periodontal diseases. Periodontol. 2000 5:66–77
    [Google Scholar]
  17. Morris M. L., Andrews R. H., Rogers A. H. 1996; The use of allozyme electrophoresis to assess genetic heterogeneity among previously subspeciated isolates of Fusobacterium nucleatum. Oral Microbiol. Immunol. 11:15–21
    [Google Scholar]
  18. Nørrung B., Gerner-Smidt P. 1993; Comparison of multilocus enzyme electrophoresis (MEE), ribotyping, restriction enzyme analysis (REA) and phage typing for typing of Listeria monocytogenes. Epidemiol. Infect. 111:71–79
    [Google Scholar]
  19. Richardson B. J., Baverstock P. R., Adams M. 1986 Allozyme electrophoresis: a handbook for animal systematics and population studies Academic Press; Sydney, Australia:
    [Google Scholar]
  20. Seiander R. K. 1980; Genetic diversity and structure in Escherichia coli populations. Science 210:545–547
    [Google Scholar]
  21. Seiander R. K., Korhonen T. K., Väisänen-Rhen V., Williams P. H., Pattison P. E., Caugant D. A. 1986; Genetic relationship and clonal structure of strains of Escherichia coli causing neonatal septicemia and meningitis. Infect. Immun. 52:213–222
    [Google Scholar]
  22. Seiander R. K., McKinney R. M., Whittam T. S., Bibb W. F., Brenner D. J., Nolte F. S., Pattison P. E. 1985; Genetic structure of populations of Legionella pneumophila. J. Bacteriol. 163:1021–1037
    [Google Scholar]
  23. Seiander R. K., Caugant D. A., Ochman H., Musser J. M., Gilmour M. N., Whittam T. S. 1986; Methods of multilocus enzyme electrophoresis for bacterial population genetics and systematics. Appl. Environ. Microbiol. 51:873–884
    [Google Scholar]
  24. Xie H., Gibbons R. J., Hay D. I. 1991; Adhesive properties of strains of Fusobacterium nucleatum of the subspecies nucleatum, vincentii and polymorphum. Oral Microbiol. Immunol. 6:257–263
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-47-1-103
Loading
/content/journal/ijsem/10.1099/00207713-47-1-103
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error